We narrowed to 13,583 results for: TIM

1. Lenti_stgRNA-Puro

   Plasmid

   #164124

   Purpose

   Backbone for lentiviral stgRNA library construction (library 1 and 2)

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJ414 - DANCER-1

   Plasmid

   #136385

   Purpose

   Expresses DANCER-1 dynamic protein

   Depositor

   Roberto Chica

   Article

   Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

   Insert

   DANCER-1
   

   Use

   Tags

   6x-His tag (N-term of insert)

   Expression

   Bacterial

   Mutation

   Mutation from GB1 WT: Y3F-L5A-L7I-A34F-V39L-V54I

   Promoter

   T7 promoter

   Available since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-QUASM1:GFPNLS-SV40pA

   Plasmid

   #155116

   Purpose

   Middle entry vector containing QUASM1 element upstream of GFP-NLS.

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   QUASM1:GFPNLS
   

   Use

   Gateway middle entry vector

   Tags

   Expression

   Mutation

   Promoter

   QUASM1-E1b

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKM54

   Plasmid

   #154157

   Purpose

   Firefly luciferase under the control of 8 copies of the Aedes aegypti Rel2 NF-κB site

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   8 copies of the Aedes aegypti Rel2 NF-κB site

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKM44

   Plasmid

   #154153

   Purpose

   Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Drosophila melanogaster minimal Hsp70 promoter

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMIII-Neo-AM

   Plasmid

   #153427

   Purpose

   Retroviral (MSCV) expression of ametrine fluorescent protein

   Depositor

   Maki Nakayama

   Article

   Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

   Insert

   ametrine
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMIII-Neo-LO

   Plasmid

   #153428

   Purpose

   Retroviral (MSCV) expression of LSSmOrange fluorescent protein

   Depositor

   Maki Nakayama

   Article

   Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

   Insert

   LSSmOrange
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYFP-x-10

   Plasmid

   #140143

   Purpose

   Evaluating the impact of high sensor concentration on information transmission of a genetic sensor.

   Depositor

   Drew Tack

   Article

   Sarkar et al Commun Biol. 2020 Apr 30;3(1):203. doi:

   Insert

   lacY (lacY )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D37, I38, and N39 are changed to nonsense (stop) …

   Promoter

   none

   Available since

   April 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYFP-x-.008

   Plasmid

   #140141

   Purpose

   Evaluating the impact of low sensor concentration on information transmission of a genetic sensor.

   Depositor

   Drew Tack

   Article

   Sarkar et al Commun Biol. 2020 Apr 30;3(1):203. doi:

   Insert

   lacY (lacY )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D37, I38, and N39 are changed to nonsense (stop) …

   Promoter

   Available since

   April 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FoxFTVC+bpFOG_MRAS-G22V

    Plasmid

    #107521

    Purpose

    Constitutive activation of FGF/MAPK pathway by M-Ras-G22V mutant

    Depositor

    Lionel Christiaen

    Article

    Razy-Krajka et al Elife. 2018 Feb 6;7. pii: 29656. doi: 10

    Insert

    M-Ras protein
    

    Use

    Tags

    Expression

    Mutation

    changed Glycine 22 to Valine

    Promoter

    TVC-specific FoxF enhancer (FoxFTVC+bpFOG)

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. BII-C3H

    Plasmid

    #133386

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. BII-B3HTK

    Plasmid

    #133389

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pQlinkH-YbATP-grasp

    Plasmid

    #129639

    Purpose

    Expression plasmid of ATP-grasp from Y. bercovieri

    Depositor

    Matthias Bochtler

    Article

    Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

    Insert

    YbATP-grasp
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    Promoter

    pQE

    Available since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. LC8-like

    Plasmid

    #133115

    Purpose

    Human LC8-like protein, codon optimised for Sf9 expression.

    Depositor

    Andrew Carter

    Article

    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

    Insert

    LC8-like (LOC392067 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Codon optimised for Sf9 expression

    Promoter

    Available since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMET17p-CCG 316

    Plasmid

    #131166

    Purpose

    For ScMET17 promoter regulated expression of mCherry-Cub-R-GFP (CCG) fused bait proteins, centromeric ARS plasmid, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant

    Depositor

    Nils Johnsson

    Article

    Dunkler et al Methods Mol Biol. 2015;1346:151-68. doi:

    Type

    Empty backbone

    Use

    Tags

    CCG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. KA1270_pPB-gPou5f1_3.5kb-UBCmin-IP

    Plasmid

    #124181

    Purpose

    piggyBac-based reporter (Puro) driven by the Pou5f1 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    IRES-Pac, Pou5f1 enhancer
    

    Use

    Reporter

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. KA2896_pPB-TREtight-Tkmin-cHA-IRESH2BmKO2

    Plasmid

    #124175

    Purpose

    piggyBac-based Dox-inducible expression (very weak)

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. KA1265_pPB-gPou5f1_3.5kb-UBCminEGFP-IP

    Plasmid

    #124180

    Purpose

    piggyBac-based reporter (EGFP) driven by the Pou5f1 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    EGFP-IRES-Pac, Pou5f1 enhancer
    

    Use

    Reporter

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-ePsuACR-mRuby2-ST

    Plasmid

    #109133

    Purpose

    Anion channelrhodopsin PsuACR fused to ER export signal and fluorophore mRuby2 and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    ePsuACR-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-eiC++-mRuby2-ST

    Plasmid

    #109132

    Purpose

    Artificial anion channelrhodopsin iC++ fused to ER export signal and fluorophore mRuby2 and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    eiC++-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAG-CsChR-mRuby2-ST

    Plasmid

    #109130

    Purpose

    Cation channelrhodopsin CsChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    CsChR-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1-R1ACR_6367-EYFP

    Plasmid

    #111080

    Purpose

    Anion channelrhodopsin from Rhodomonas sp. for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin R1ACR_6367
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-RlACR_497-EYFP

    Plasmid

    #111078

    Purpose

    Anion channelrhodopsin from Rhodomonas lens for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin RlACR_497
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-NMBR-eLOV-TEVcs-FLAG-GAL4-V5

    Plasmid

    #104843

    Purpose

    b2AR-SPARK TF component

    Depositor

    Alice Ting

    Article

    Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

    Insert

    NMBR-eLOV-TEVcs-FLAG-GAL4-V5
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pG-HIV-SIN-2LTR

    Plasmid

    #104591

    Purpose

    Standard for qPCR

    Depositor

    Kristine Yoder

    Article

    Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

    Insert

    2LTR circle junction of self-inactivating HIV-based vectors
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAM-PAT-ProTTG1 GW

    Plasmid

    #79753

    Purpose

    binary DEST vector for plant expression

    Depositor

    Nico Dissmeyer, Martin Hulskamp

    Article

    Bouyer et al PLoS Biol. 2008 Jun 10;6(6):e141. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    ProTTG1

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSUPER-retro-puro-shNup88-XbaI

    Plasmid

    #87328

    Purpose

    To express shRNA against human Nup88

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    shRNA against human Nup88
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSUPER-retro-puro-shNup93-XhoI

    Plasmid

    #87330

    Purpose

    To express shRNA against human Nup93

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    shRNA against human Nup93
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pILGFPB6

    Plasmid

    #83557

    Purpose

    Used to evaluate the expression output of GAL10 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    GAL10 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDA186

    Plasmid

    #74250

    Purpose

    Intermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pGPD1 (UbiY 2xNLSs SZ3).

    Depositor

    Serge Pelet

    Article

    Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

    Insert

    Inducible peptide of the dPSTR
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    pGPD1

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge