We narrowed to 6,013 results for: cki;

1. 6K-loop-->R GLUT1-GFP

   Plasmid

   #200098

   Purpose

   human GLUT1 with K-->R mutation of 6 lysines in the major cytoplasmic loop and C-terminal GFP tag in pQCXIP vector

   Depositor

   Jason MacGurn

   Article

   Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

   Insert

   GLUT1 (SLC2A1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   K-->R mutations of amino acids 225, 229, 230, …

   Promoter

   CMV

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 11K-cyto-->R GLUT1-GFP

   Plasmid

   #200096

   Purpose

   human GLUT1 with K-->R mutations for all 11 cytosolic lysines and a C-terminal GFP tag in pQCXIP vector

   Depositor

   Jason MacGurn

   Article

   Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

   Insert

   GLUT1 (SLC2A1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   K-->R mutations of amino acids 6, 7, 225, 229,…

   Promoter

   CMV

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. WDR5-HaloTag

   Plasmid

   #200881

   Purpose

   Mammalian expression of human WDR5 with C-terminal HaloTag fusion

   Depositor

   Stefan Knapp

   Article

   Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

   Insert

   WDR5-HaloTag (WDR5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-PGK-EGFPc3-Neo

   Plasmid

   #189741

   Purpose

   The construct was used to express GFP in mammalian cells as the control for the analysis of the subcellular location of this protein.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-PSAT

   Plasmid

   #74115

   Purpose

   Expression of PSAT

   Depositor

   Lewis Cantley

   Article

   Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

   Insert

   pET28a-PSAT (PSAT1 Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5/FRT/TO-B2AR-APEX

   Plasmid

   #96848

   Purpose

   For expression of B2AR-APEX in mammalian cells

   Depositor

   Andrew Kruse

   Article

   Paek et al Cell. 2017 Apr 6;169(2):338-349.e11. doi

   Insert

   B2AR-APEX (ADRB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GFP-FKBP-TPD54

   Plasmid

   #172454

   Purpose

   Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal GFP-FKBP tag

   Depositor

   Stephen Royle

   Article

   Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

   Insert

   TPD54 (TPD52L2 Human)
   

   Use

   Tags

   GFP-FKBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJW1582

   Plasmid

   #154312

   Purpose

   mNeonGreen^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   mNeonGreen^SEC^AID*::3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GFP-P2A-CIAR_pLenti_CMV_Puro

   Plasmid

   #86503

   Purpose

   Lenti plasmid for generating CIAR expressing stable cell lines

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

   Insert

   GFP-P2A-CIAR (SOS1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   T968L in SOScat

   Promoter

   CMV

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLentiCRISPRv4

    Plasmid

    #221432

    Purpose

    Lentiviral CRISPR/Cas9 vector for co-expression of sgRNAs and Cas9 protein.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEMS1943

    Plasmid

    #82565

    Purpose

    High copy number plasmid containing Ple266 (S100B MiniPromoter) insert.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple266
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pEMS1997

    Plasmid

    #49127

    Purpose

    AAV plasmid with SLC6A4 (Ple198) promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-Ple198-iCre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SLC6A4

    Available since

    May 7, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pTRE-TIGHT-Cx43-eYFP

    Plasmid

    #31807

    Depositor

    Robin Shaw

    Article

    Smyth et al J Clin Invest. 2010 Jan 4. 120(1):266-79

    Insert

    Cx43 (GJA1 Human)
    

    Use

    Tags

    eYFP

    Expression

    Mammalian

    Mutation

    Promoter

    P-TIGHT

    Available since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

14. mVenusC-Cavin1

    Plasmid

    #108888

    Purpose

    Expression of Cavin1 fused to the C-terminus of mVenus for bimolecular fluorescence complementation

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    Cavin1 (altered codon usage) (CAVIN1 Human, Synthetic)
    

    Use

    Tags

    mVenus156-239

    Expression

    Mammalian

    Mutation

    altered codon usage

    Promoter

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEMS2143

    Plasmid

    #104356

    Purpose

    AAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporter. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-smCBA-EmGFP-WPRE
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    smCBA

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. His-ATG3

    Plasmid

    #202777

    Purpose

    ATG3 protein (WT) overexpression in E.Coli. 6His-TEVsite-ATG3 WT-stop

    Depositor

    James Hurley

    Article

    Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

    Insert

    ATG3 (ATG3 Human)
    

    Use

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMMDS DEST

    Plasmid

    #206264

    Purpose

    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. After LR propagate in Pir+. Works as a CRE-donor in the MultiBac system.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Type

    Empty backbone

    Use

    Cre-donor, multimate/gateway dest vector (multiba…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJW1838

    Plasmid

    #154324

    Purpose

    sgRNA Destination Vector for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    SapTrap sgRNA (F+E) vector, K09B11.2 U6 promoter and 3'UTR
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    F+E mutations in sgRNA

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRK5-myc-Miro1 S432N

    Plasmid

    #47893

    Purpose

    Expresses myc tagged Miro1 S432N dominant negative mutant

    Depositor

    Pontus Aspenström

    Article

    Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

    Insert

    Miro1 S432N (RHOT1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S432N, dominant negative mutant

    Promoter

    CMV

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

20. 1K245 GLUT1-GFP

    Plasmid

    #200097

    Purpose

    human GLUT1 with a single cytosolic lysine (K245) and C-terminal GFP tag in pQCXIP vector

    Depositor

    Jason MacGurn

    Article

    Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

    Insert

    GLUT1 (SLC2A1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K-->R mutations of amino acids 6, 7, 225, 229,…

    Promoter

    CMV

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJW2088

    Plasmid

    #154336

    Purpose

    Crispr repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::GFP::3xFLAG::10aa linker
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTag-RFP-C-h-Rab4a-c-Myc

    Plasmid

    #79800

    Purpose

    Express TagRFP labeled human Rab5a (GTPase located on recycling endosomal membranes).

    Depositor

    James Johnson

    Article

    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

    Insert

    Rab4a (RAB4A Human)
    

    Use

    Tags

    TagRFP and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pEMS2031

    Plasmid

    #49134

    Purpose

    AAV plasmid with OLIG1 (Ple305) promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-Ple305-iCre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    OLIG1

    Available since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. 2xFKBP-smFP(myc)-KDEL

    Plasmid

    #173021

    Purpose

    FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of spaghetti monster FP (myc, non-fluorescent). IRES following ORF.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    2xFKBP-smFP(myc)-KDEL
    

    Use

    Tags

    FKBP and smFP(myc) non-fluorescent

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJW1846

    Plasmid

    #154326

    Purpose

    Multi-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::GFP^SEC^AID*::3xFLAG
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    F+E mutations in sgRNA

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mito-yHS1-M7A

    Plasmid

    #159164

    Purpose

    Mitochondrial labile heme reporter

    Depositor

    Amit Reddi

    Article

    Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

    Insert

    mito-yHS1-M7A
    

    Use

    Tags

    COX4 pre-sequence

    Expression

    Yeast

    Mutation

    Mutated Met 7 of the cyt b562 module of mito-yHS1…

    Promoter

    GPD

    Available since

    Sept. 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pcDNA3.1-mGnptg

    Plasmid

    #78106

    Purpose

    Mammalian expression of mouse Gnptg with C-terminal His tag

    Depositor

    Thomas Braulke

    Article

    Encarnacao et al J Biol Chem. 2011 Feb 18;286(7):5311-8.

    Insert

    Gnptg (Gnptg Mouse)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

28. pRS313_ATP3T911A

    Plasmid

    #120254

    Purpose

    Expression of ATP3-6 in yeast

    Depositor

    Markus Ralser

    Article

    Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

    Insert

    ATP3 (ATP3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T911 changed to A; Isoleucine 304 changed to Aspa…

    Promoter

    ATP3

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV asyn S87A

    Plasmid

    #36056

    Depositor

    Hilal Lashuel

    Article

    Oueslati et al J Neurosci. 2012 Feb 1;32(5):1536-44.

    Insert

    alpha-synuclein S87A (SNCA Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S87A

    Promoter

    CMV

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

30. Mutant Human Cortactin-GFP

    Plasmid

    #71365

    Purpose

    mammalian expression of mutant human Cortactin-GFP

    Depositor

    Alpha Yap

    Article

    Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

    Insert

    cortactin (CTTN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    silent mutation present at 72nd nucleotide

    Promoter

    CMV

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only