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p361_DG

Plasmid

#234683

Purpose

Golden gate domesticated pET28b with His6-3C-3XFLAG-SUMO and Thrombin-HiBiT

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His6-3C-3XFLAG-SUMO and Thrombin-HiBiT

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pSB1A3_GFP_dT25

Plasmid

#89664

Purpose

expression of doubleTAL staple protein dT25 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT25

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSB1A3_GFP_dT19

Plasmid

#89661

Purpose

expression of doubleTAL staple protein dT19 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT19

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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mScarlet-Rab9aS21N

Plasmid

#169073

Purpose

Express human dominant negative form of Rab9a(S21N) in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rab9a (RAB9A Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-LUCI-GECO1

Plasmid

#113675

Purpose

Genetically-encoded ratiometric bioluminescent Ca2+ indicator based on GCaMP6s

Depositor

Robert Campbell

Article

Qian et al Chembiochem. 2018 Jun 22. doi: 10.1002/c

Insert

LUCI-GECO1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-mYFP-hphMX6

Plasmid

#105155

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mYFP-hphMX6

Tags

mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pTK750

Plasmid

#114718

Purpose

Express mCherry-NuMA(5A-3) from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA 5A-3

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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SA-betageo

Plasmid

#21709

Depositor

Philippe Soriano

Article

Friedrich et al Genes Dev. 1991 Sep . 5(9):1513-23.

Insert

SAbgeo

Use

Mouse gene trapping

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pEnt R3L2 TetO(fl)-2

Plasmid

#24416

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Type

Empty backbone

Use

Entry vector

Available Since

April 1, 2011

Availability

Academic Institutions and Nonprofits only

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pCAG:myr-Venus

Plasmid

#32602

Depositor

Anna-Katerina Hadjantonakis

Article

Rhee et al Genesis. 2006 Apr;44(4):202-18.

Insert

myr-Venus

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
mEosEM-E_N1

Plasmid

#226337

Purpose

Photoswitchable fluorescent protein for correlative light-electron microscopy; empty backbone containing mEosEM-E for mammalian expression

Depositor

Pingyong Xu

Article

Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

Type

Empty backbone

Expression

Mammalian

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMyc-N1

Plasmid

#85759

Purpose

A mammalian expression vector with a Myc-tag at C-terminus.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Type

Empty backbone

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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TAL2065

Plasmid

#39435

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-39-Right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
TAL2064

Plasmid

#39434

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-39-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
pTK586

Plasmid

#114716

Purpose

SNAP-tagging at LGN's N-terminal locus (BSD)

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

SNAP

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTK503

Plasmid

#114717

Purpose

Express mCherry-NuMA(WT) from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pXZ13lacZalpha

Plasmid

#160229

Purpose

places LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal plates

Depositor

Ian Duncan

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

lac

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-600uDelta13V

Plasmid

#18014

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16A Delta13

Tags

ECFP and Venus

Expression

Mammalian

Mutation

F16A in binding protein

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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Venus-giantin

Plasmid

#234749

Purpose

Mammalian expression of Golgi marker Venus-giantin

Depositor

Nevin Lambert

Article

Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

Insert

Venus-giantin

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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Venus-MOA

Plasmid

#234741

Purpose

Mammalian expression of mitochondria marker Venus-MOA

Depositor

Nevin Lambert

Article

Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

Insert

Venus-MOA

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a- mYFP-kanMX6

Plasmid

#105147

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mYFP-kanMX6

Tags

mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pXJ40-HA-Merlin I

Plasmid

#19699

Depositor

Filippo Giancotti

Article

Okada et al J Cell Biol. 2005 Oct 24. 171(2):361-71.

Insert

Merlin I (Nf2 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only
lvl0 alcSynth-34S

Plasmid

#153411

Purpose

Ethanol system inducible promoter

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

alcSynth-34S promoter

Expression

Plant

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG:Kaede

Plasmid

#39261

Depositor

Anna-Katerina Hadjantonakis

Article

Nowotschin et al BMC Dev Biol. 2009 Sep 9;9:49.

Insert

Kaede

Expression

Mammalian

Promoter

pCAGGS

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only
pTPTP alpha

Plasmid

#17691

Depositor

Xinmin Zheng

Article

Zheng et al EMBO J. 2000 Mar 1. 19(5):964-78.

Insert

PTPalpha (PTPRA Human)

Tags

HA

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
p367_DG

Plasmid

#234689

Purpose

Golden gate domesticated pET28b with His10-bdNEDD8 and 2X Strep tag

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

2X Strep tag and His10-bdNEDD8

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p360_DG

Plasmid

#234703

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-mNeongreen-bdNEDD8

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-mNeongreen-bdNEDD8

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL2038

Plasmid

#39396

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-20-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
TAL2039

Plasmid

#39397

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-20-Right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
Reb_81 aka PAS622

Plasmid

#71227

Purpose

Reb_1 with RebB(T28A, D72G)

Depositor

Pamela Silver

Article

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

none

Expression

Bacterial

Mutation

RebB(T28A, D72G), RebA(G53A)

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
pCS2 xenopus sizzled

Plasmid

#24684

Depositor

Edward De Robertis

Article

Lee et al Cell. 2006 Jan 13. 124(1):147-59.

Insert

Sizzled (szl Frog)

Expression

Mammalian

Available Since

July 22, 2010

Availability

Academic Institutions and Nonprofits only
pPyCAG-HA-p110-IP

Plasmid

#15691

Depositor

Shinya Yamanaka

Article

Takahashi et al Nature. 2003 May 29. 423(6939):541-5.

Insert

p110 delta (Pik3cd Mouse)

Tags

HA

Expression

Mammalian

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
pKH3-avian RSK1

Plasmid

#8998

Depositor

John Blenis

Article

Richards et al Mol Cell Biol. 2001 Nov . 21(21):7470-80

Insert

RSK (RPS6KA1 Chicken)

Tags

3x HA

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
HA.PKD.Y463E

Plasmid

#10812

Depositor

Alex Toker

Article

Storz et al EMBO J. 2003 Jan 2. 22(1):109-20.

Insert

PKD (PRKD1 Human)

Tags

HA

Expression

Mammalian

Mutation

Y463E--active; P467L and L520P

Promoter

CMV

Available Since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only
Reb_207 aka PAS630

Plasmid

#71235

Purpose

Reb_206 with RebA(S88P)

Depositor

Pamela Silver

Article

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

mNeonGreen to second copy RebB, soluble mCherry a…

Expression

Bacterial

Mutation

RebA(S88P)

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-3.2mDelta13V

Plasmid

#18011

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16A S112A Delta13

Tags

ECFP and Venus

Expression

Mammalian

Mutation

F16A, S112A in binding protein

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
Kaede-SpVasa

Plasmid

#185582

Purpose

for photoconversion

Depositor

Mamiko Yajima

Article

Wavreil et al Dev Biol. 2021 Oct;478:13-24. doi: 10.10

Insert

Vasa

Use

In vitro transcription (mrna synthesis)

Tags

Kaede

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH4.52

Plasmid

#26946

Depositor

Geraldine Seydoux

Article

Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

Insert

histone H2B (his-58 Nematode)

Tags

GFP

Expression

Worm

Available Since

June 22, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-Sox15

Plasmid

#13359

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

SRY-box containing gene 15 (Sox15 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pTricHis-PLY

Plasmid

#160537

Purpose

Expression of inserted PLY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

PLY-WT

Tags

His-Tag

Expression

Bacterial

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 17,506 results for: Emb