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Showing: 4001 - 4040 of 84526 results

  1. PX459v3

    Plasmid
    #178799
    Purpose
    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178799-plasmid-map-sequence-id-354842

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  3. FUGW-PK-hLC3∆G

    Plasmid
    #61461
    Purpose
    Express pHluorin-mKate2-hLC3∆G (PK-hLC3∆G), a negative control for pHluorin-mKate2-hLC3 (PK-hLC3), in mammalian cells for monitoring autophagy, based on FUGW (3rd gen lentiviral plasmid)
    Depositor
    Isei Tanida
    Article
    Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi
    Insert
    PK-hLC3∆G (MAP1LC3B Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hUBC
    Available since
    Feb. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    61461-plasmid-map-sequence-id-100031

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  4. LV_EF1a_CDK1(T14A,Y15F)-P2A-Hygro_Barcode

    Plasmid
    #170220
    Purpose
    Barcoded lentiviral vector to express CDK1 (T14A, Y15F) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq
    Depositor
    Prashant Mali
    Article
    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi
    Insert
    CDK1 (CDK1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    T14A, Y15F
    Promoter
    EF1a
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170220-plasmid-map-sequence-id-334386

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  7. AAV-hSyn-Ribo-jGCaMP8s

    Plasmid
    #167572
    Purpose
    DEPRECATED: Please see Addgene item #169247 for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s.
    Depositor
    Marianne Fyhn
    Article
    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1
    Has Service
    AAV1
    Insert
    Ribo-GCaMP8s
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167572-plasmid-map-sequence-id-334463

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  18. AAV-hSyn-Ribo-jGCaMP8m

    Plasmid
    #167574
    Purpose
    DEPRECATED: Please see Addgene item #169248 for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m.
    Depositor
    Marianne Fyhn
    Article
    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1
    Has Service
    AAV1
    Insert
    Ribo-GCaMP8m
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167574-plasmid-map-sequence-id-334462

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  21. pCAGGS-PHFF

    Plasmid
    #100280
    Purpose
    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Insert
    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    Use
    Tags
    FLAG, T7 and Mitochondria-targeting sequence (MTS…
    Expression
    Mammalian
    Mutation
    All genes are codon-optimized for expression in h…
    Promoter
    CAG promoter
    Available since
    Sept. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    100280-plasmid-map-sequence-id-194335

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  25. pcDNA3-sPDGFRalpha-Fc

    Plasmid
    #136438
    Purpose
    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    PDGFRalpha (PDGFRA Human)
    Use
    Tags
    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…
    Expression
    Mammalian
    Mutation
    Extracellular domain of PDGFR-alpha (a.a. 1-524)
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136438-plasmid-map-sequence-id-271697

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  28. EF1a_MYC deltaCTD_P2A_Hygro_Barcode

    Plasmid
    #120511
    Purpose
    Barcoded lentiviral vector to express MYC deltaCTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Insert
    MYC deltaCTD (MYC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Deletion of carboxy-terminal domain
    Promoter
    EF1a
    Available since
    Feb. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    120511-plasmid-map-sequence-id-234934

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  29. EF1a_MYC deltaNTD_P2A_Hygro_Barcode

    Plasmid
    #120510
    Purpose
    Barcoded lentiviral vector to express MYC deltaNTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Insert
    MYC deltaNTD (MYC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Deletion of amino-terminal domain
    Promoter
    EF1a
    Available since
    Feb. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    120510-plasmid-map-sequence-id-234933

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  30. EF1a_MYC deltaMBI_P2A_Hygro_Barcode

    Plasmid
    #120504
    Purpose
    Barcoded lentiviral vector to express MYC deltaMBI in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Insert
    MYC deltaMBI (MYC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Deletion of MYC Box I domain
    Promoter
    EF1a
    Available since
    Feb. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    120504-plasmid-map-sequence-id-234804

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  32. LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode

    Plasmid
    #170240
    Purpose
    Barcoded lentiviral vector to express MEK1 (S218D, S222D) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq
    Depositor
    Prashant Mali
    Article
    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi
    Insert
    MAP2K1 (MAP2K1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    S218D, S222D
    Promoter
    EF1a
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170240-plasmid-map-sequence-id-334554

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  34. EF1a_MYC deltab_P2A_Hygro_Barcode

    Plasmid
    #120507
    Purpose
    Barcoded lentiviral vector to express MYC deltab in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Insert
    MYC deltab (MYC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Deletion of basic motif
    Promoter
    EF1a
    Available since
    Feb. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    120507-plasmid-map-sequence-id-234929

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  35. EF1a_MYC deltaMBII_P2A_Hygro_Barcode

    Plasmid
    #120505
    Purpose
    Barcoded lentiviral vector to express c-MYC deltaMBII in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Insert
    c-MYC deltaMBII (MYC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Deletion of MYC Box II domain
    Promoter
    EF1a
    Available since
    Oct. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    120505-plasmid-map-sequence-id-234918

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  36. EF1a_MYC deltaNLS_P2A_Hygro_Barcode

    Plasmid
    #120506
    Purpose
    Barcoded lentiviral vector to express c-MYC deltaNLS in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Insert
    MYC H84NLS (MYC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Deletion of nuclear localization signal sequence
    Promoter
    EF1a
    Available since
    Oct. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    120506-plasmid-map-sequence-id-234926

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  38. dCas9-NLS-gs10-cLYN268

    Plasmid
    #188259
    Purpose
    Plasmid ensures constitutive expression of the C-terminal fragment of split LYN kinase domain (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
    Depositor
    Roman Jerala
    Article
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Insert
    cLYN268 (LYN Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    188259-plasmid-map-sequence-id-374580

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  39. AAV-hSyn-Ribo-jGCaMP8f

    Plasmid
    #167573
    Purpose
    DEPRECATED: Please see Addgene item #169249 (coming soon) for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f.
    Depositor
    Marianne Fyhn
    Article
    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1
    Insert
    Ribo-GCaMP8f
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167573-plasmid-map-sequence-id-334459

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Showing: 4001 - 4040 of 84526 results