We narrowed to 12,311 results for: BASE

1. pENTR-L1-PGK-HygTK-L2

   Plasmid

   #141006

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   HygTK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKAd5f11pES-PmeI

   Plasmid

   #122698

   Purpose

   To generate an E1/E3-deleted human adenovirus 5 (HAdV-5) bearing a chimeric fiber and the exogenous gene expression cassette.

   Depositor

   Zhuo-Zhuang Lu

   Article

   Liu et al Hum Gene Ther. 2019 Feb 22. doi: 10.1089

   Type

   Empty backbone

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKAd5f11p-EPG

   Plasmid

   #122697

   Purpose

   To generate E1/E3-deleted human adenovirus 5 (HAdV-5) bearing a chimeric fiber and an EF1a promoter-controlled transgene.

   Depositor

   Zhuo-Zhuang Lu

   Article

   Liu et al Hum Gene Ther. 2019 Feb 22. doi: 10.1089

   Insert

   GFP
   

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. tagSrc-HaloTag

   Plasmid

   #179141

   Purpose

   The tag is SsrA (AANDENY). Tag is inserted after N534 of Src.

   Depositor

   Klaus Hahn

   Article

   Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

   Insert

   Src, SsrA
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKAd5f11pABR-EPG

   Plasmid

   #122699

   Purpose

   It can be used to construct a recombinant virus carrying other transgene or an adenovector with modified fiber.

   Depositor

   Zhuo-Zhuang Lu

   Article

   Liu et al Hum Gene Ther. 2019 Feb 22. doi: 10.1089

   Insert

   GFP
   

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. BFP Homology Donor

   Plasmid

   #149372

   Purpose

   Promoter-less BFP cDNA with two homology arms to the lenti-CDDR reporter for HDR detection

   Depositor

   Tarek Abbas

   Article

   Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

   Insert

   CDDR BFP Homology Donor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. p2T-CAG-REV1-p2A-GFP-PuroR

   Plasmid

   #153001

   Purpose

   Overexpression of human REV1

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   REV1 (REV1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBT138b-proD

   Plasmid

   #138516

   Purpose

   P3e

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   T7-RNAP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGR-1 TurboRFP

   Plasmid

   #52306

   Purpose

   Fluid Shear Stress inducible Red Fluorescent Protein for mammalian expression

   Depositor

   Joel Voldman

   Article

   Varma et al Lab Chip. 2015 Feb 4.

   Insert

   EGR-1 Promoter (Egr1 Mouse)
   

   Use

   Tags

   TurboRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLb-Cpf1-pGL3-U6-sgRNA

    Plasmid

    #107682

    Purpose

    Expresses LbCpf1 crRNA in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEN_Tmcs

    Plasmid

    #25751

    Purpose

    Entry vector with TRE promoter followed by multiple cloning site.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTS93 pHAGE2 CMVtetO2 GFP-22xGS-MCS

    Plasmid

    #199346

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pM201

    Plasmid

    #173179

    Purpose

    dCas9-24xGCN4

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    dCas9-24xGCN4
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. AcpP pet29a C-terminal His Tag

    Plasmid

    #75016

    Purpose

    Expresses the acyl carrier protein (ACP) from the E. coli fatty acid synthase

    Depositor

    Michael Burkart

    Article

    Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

    Insert

    AcpP (acpP E. coli)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pDEST26-USP47

    Plasmid

    #64326

    Purpose

    Mammalian expression and purification of USP47.

    Depositor

    Grigory Dianov

    Article

    Parsons et al Mol Cell. 2011 Mar 4;41(5):609-15. doi:

    Insert

    USP47 (USP47 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pN-PITCh-3XHA

    Plasmid

    #127881

    Purpose

    Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HA tagging

    Depositor

    Peter Kaiser

    Article

    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

    Insert

    GFP-Puro-3XHA (PPP2CA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. T. aq RNA Polymerase

    Plasmid

    #215349

    Purpose

    Express T. aq RNA Polymerase subunits in E. coli cells

    Depositor

    Julius Lucks

    Article

    Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

    Insert

    RNA polymerase
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pegRNA-GBA-10

    Plasmid

    #199271

    Purpose

    pegRNA used to induce GBA (c. 1226 A > G) mutation

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    GBA 1226AtoG pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Twin-Strep(R)-Tev-GSGS-Ubiquitin

    Plasmid

    #186803

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses Twin-Strep(R)-Tev-GSGS-Ubiquitin.

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    Twin-Strep(R)-Tev-GSGS-Ubiquitin
    

    Use

    Tags

    GS-Strep (WSHPQFER)-GGGSGGGSGGSSA-Strep (WSHPQFER…

    Expression

    Bacterial

    Mutation

    Strep tag Lysine's mutated to Arginine

    Promoter

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Tol2-cmlc2-Trpa1b-2A-EGFP

    Plasmid

    #106405

    Purpose

    For expressing Trpa1b-2A-EGFP in zebrafish cardiomyocytes.

    Depositor

    Randall Peterson

    Article

    Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

    Inserts

    cmlc2 promoter
    Trpa1b (trpa1b Zebrafish)
    

    Use

    Gateway

    Tags

    2A-EGFP

    Expression

    Mutation

    Promoter

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET-Sfp R4-4 mutant

    Plasmid

    #173491

    Purpose

    Expression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.

    Depositor

    Jun Yin

    Article

    Sunbul et al J Mol Biol. 2009 Apr 10;387(4):883-98. d

    Insert

    Sfp-R44
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. ZJOM160

    Plasmid

    #133670

    Purpose

    Integration of mTagBFP2-SKL as an additional copy in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast peroxisome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    pTPI1-mTagBFP2-SKL
    

    Use

    Tags

    mTagBFP2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLPR2

    Plasmid

    #209980

    Purpose

    Contains Level 0 Part: resistance cassette (cat) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    resistance cassette (cat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLPC54

    Plasmid

    #209983

    Purpose

    Contains Level 0* Part: 5' Connector (5C_07_5C1C) for the construction of Level 2 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    5' Connector (5C_07_5C1C)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLPC33

    Plasmid

    #209984

    Purpose

    Contains Level 0* Part: 3' Connector (3C_08_3C3) for the construction of Level 2 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    3' Connector (3C_08_3C3)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLPR3

    Plasmid

    #209986

    Purpose

    Contains Level 0* Part: resistance cassette (ermBL) for the construction of Level 2 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    resistance cassette (ermBL)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLPO5

    Plasmid

    #209987

    Purpose

    Contains Level 0* Part: replication origin (256rep) for the construction of Level 2 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    replication origin (256_rep)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLPP3

    Plasmid

    #209965

    Purpose

    Contains Level 0 Part: constitutive Promoter (P25) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Promoter (P25)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLPP7

    Plasmid

    #209969

    Purpose

    Contains Level 0 Part: autoinducible Promoter (PfabZ) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Promoter (P_fabZ)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLPP8

    Plasmid

    #209970

    Purpose

    Contains Level 0 Part: autoinducible Promoter (PgadB) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Promoter (P_gadB)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLPP9

    Plasmid

    #209971

    Purpose

    Contains Level 0 Part: constitutive Promoter (PldhD) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Promoter (P_ldhD)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLPS2

    Plasmid

    #209972

    Purpose

    Contains Level 0 Part: ribosome binding site (L. plantarum optimized) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    RBS (Lp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLPT2

    Plasmid

    #209974

    Purpose

    Contains Level 0 Part: Terminator (TpepN) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Terminator (T_pepN)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLPR1

    Plasmid

    #209979

    Purpose

    Contains Level 0 Part: resistance cassette (ermBL) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    resistance cassette (ermBL)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLP3

    Plasmid

    #209958

    Purpose

    Entry vector to store Level 0 broad-host range L. plantarum replication origin parts

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLPP1

    Plasmid

    #209963

    Purpose

    Contains Level 0 Part: mock / negative control Promoter (P-nc) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Promoter (mock)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLPP2

    Plasmid

    #209964

    Purpose

    Contains Level 0 Part: constitutive Promoter (P48) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Promoter (P48)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pNeoXTR f0

    Plasmid

    #69157

    Purpose

    mouse targeting vector

    Depositor

    David Feldser

    Article

    Robles-Oteiza et al Nat Commun. 2015 Nov 5;6:8783. doi: 10.1

    Inserts

    DTA
    NeoR
    GFP
    AmpR
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Amp promoter, PGK, and none

    Available since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSW002-PpsbA_mTurquoise2

    Plasmid

    #108237

    Purpose

    Broad host bacterial expression vector with constitutive PsbA promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    PsbA promoter - mTurquoise2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. 3xFLAG-dCas9/pMSCVneo

    Plasmid

    #134982

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge