8,734 results

1. pEGFP-Nup88

   Plasmid

   #64283

   Purpose

   GFP-tagged mammalian expression of full-length Nup88

   Depositor

   Birthe Fahrenkrog

   Article

   Lussi et al Mol Biol Cell. 2011 Apr;22(7):1080-90. d

   Insert

   Nup88 (NUP88 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pMA4008

   Plasmid

   #70109

   Purpose

   mtDNA destruction/production of rho-0 cells

   Depositor

   Mikhail Alexeyev

   Article

   Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

   Inserts

   EGFP
   Herpes simplex virus UL12.5M185
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   N-terminal deletion of UL12.5

   Promoter

   CMV and RSV

   Available since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-DIO-hM3D(Gq)-mCherry

   Plasmid

   #44361

   Purpose

   Double floxed Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter.

   Depositor

   Bryan Roth

   Article

   Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   hM3D(Gq)-mCherry
   

   Use

   AAV; Adeno associated viral vector

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS306-URA3-PHIS3-AqLumSynth –Sapphire

   Plasmid

   #116931

   Purpose

   Yeast integrative vector for the exression of the 20nm GEM under the HIS3 promoter

   Depositor

   Liam Holt

   Article

   Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

   Insert

   lumazine synthase
   

   Use

   Tags

   Sapphire

   Expression

   Yeast

   Mutation

   Promoter

   HIS3

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. aeBlue chromoprotein

   Plasmid

   #117846

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses aeBlue chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, aeBlue
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCLBW cox8 EGFP mCherry

   Plasmid

   #78520

   Purpose

   Fluorescent mitophagy reporter

   Depositor

   David Chan

   Article

   Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

   Insert

   none
   

   Use

   Retroviral

   Tags

   cox8 EGFP mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYLCRISPR/Cas9Pubi-B

   Plasmid

   #66188

   Purpose

   expression of Cas9 in plants, maize ubiquitin promoter (Pubi), basta selection

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Plant

   Mutation

   plant-codon optimized with higher GC contents (62…

   Promoter

   maize ubiquitin promoter (Pubi)

   Available since

   Sept. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP NFAT5 (no EGFP)

   Plasmid

   #13627

   Depositor

   Anjana Rao

   Article

   Lopez-Rodriguez et al Proc Natl Acad Sci U S A. 1999 Jun 22. 9

   Insert

   NFAT5 (NFAT5 Human)
   

   Use

   Tags

   6xMyc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET His6 TEV LIC cloning vector (1B)

   Plasmid

   #29653

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. p5E-elavl3

    Plasmid

    #75025

    Purpose

    Can be used to drive pan neuronal expression in zebrafish.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    ELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Tags

    Expression

    Mutation

    Promoter

    elavl3

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti PGK Puro DEST (w529-2)

    Plasmid

    #19068

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Puro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZW1-snoVector

    Plasmid

    #73174

    Purpose

    Nuclear expression of RNAs

    Depositor

    Ling-Ling Chen

    Article

    Yin et al Nucleic Acids Res. 2015 Jan;43(1):e5. do

    Inserts

    SNORD116-13 (SNORD116-13 Human)
    SNORD116-14 (SNORD116-14 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEM-3z/601

    Plasmid

    #26656

    Depositor

    Jonathan Widom

    Article

    Lowary et al J Mol Biol. 1998 Feb 13. 276(1):19-42.

    Insert

    
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-VSVG

    Plasmid

    #35616

    Depositor

    Patrick Salmon, Arthur Nienhuis

    Insert

    VSV G protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHL-sec

    Plasmid

    #99845

    Purpose

    Mammalian expression with secretion signal sequence

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    ephrin b2 (EFNB2 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1-Neo-M2rtTA

    Plasmid

    #60843

    Purpose

    AAVS1 donor vector for genomic targeting

    Depositor

    Rudolf Jaenisch

    Article

    DeKelver et al Genome Res. 2010 Aug;20(8):1133-42. doi:

    Inserts

    M2rtTA
    Neo
    

    Use

    Targeting donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR_PGK

    Plasmid

    #79120

    Purpose

    Lentiviral vector for constitutive expression of genes (PGK promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3β-FLAG-CBP-HA

    Plasmid

    #32908

    Depositor

    Tso-Pang Yao

    Article

    Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

    Insert

    CBP (Crebbp Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEXTK-Pak1 70-117

    Plasmid

    #12217

    Depositor

    Jonathan Chernoff

    Insert

    Pak1 (PAK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    PBD (p21-binding domain), aa 70-117

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCas9_GFP

    Plasmid

    #44719

    Purpose

    Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

    Depositor

    Kiran Musunuru

    Article

    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

    Insert

    Cas9-2A-GFP
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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