Skip to main content
Addgene
Showing: 4201 - 4240 of 84526 results

  3. pDEST-N2H-F2-empty vector

    Plasmid
    #125552
    Purpose
    N2H assay vector with nanoluc fragment2 (empty control, no Gateway cloning site, expressing nanoluc fragment2)
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment2 alone without any attached prot…
    Expression
    Mammalian and Yeast
    Mutation
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125552-plasmid-map-sequence-id-252313

    Enlarge

  4. pCAG-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1081)

    Plasmid
    #114094
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-RR (S542R/K607R)
    Use
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    S542R and K607R
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114094-plasmid-map-sequence-id-220561

    Enlarge

  5. pDEST-N2H-F1-empty vector

    Plasmid
    #125551
    Purpose
    N2H assay vector with nanoluc fragment1 (empty control, no Gateway cloning site, expressing nanoluc fragment1)
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment1 alone without any attached prot…
    Expression
    Mammalian and Yeast
    Mutation
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125551-plasmid-map-sequence-id-252311

    Enlarge

  7. pCAG-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1065)

    Plasmid
    #114092
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a (S542R/K548V/N552R)
    Use
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    S542R, K548V and N552R
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114092-plasmid-map-sequence-id-230539

    Enlarge

  8. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-gs-UGI-NLS(SV40)(AsBE1.4) (RTW1008)

    Plasmid
    #114080
    Purpose
    CAG promoter expression plasmid for human codon optimized AsBE1.4
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.4)
    Use
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114080-plasmid-map-sequence-id-230482

    Enlarge

  9. pCAG-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(LbBE1.1) (RTW1295)

    Plasmid
    #114085
    Purpose
    CAG promoter expression plasmid for human codon optimized LbBE1.1
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.1)
    Use
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114085-plasmid-map-sequence-id-230653

    Enlarge

  10. pCAG-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-3xHA (AAS826)

    Plasmid
    #114091
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a(E174R/S542R) nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a (E174R/S542R)
    Use
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    E174R and S542R
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114091-plasmid-map-sequence-id-220538

    Enlarge

  11. pCAG-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(AsBE1.1) (RTW1351)

    Plasmid
    #114079
    Purpose
    CAG promoter expression plasmid for human codon optimized AsBE1.1
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.1)
    Use
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114079-plasmid-map-sequence-id-231032

    Enlarge

  12. pMRX-IP-EGFP-PA-BAX(lyso)

    Plasmid
    #228939
    Purpose
    Stable expression of EGFP-LOV2(G528A, N538E)-BAX-TMEM106B in mammalian cells. Photoactivatable BAX localizes on lysosomal membrane and induces lysosomal rupture upon blue light stimulation.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    TMEM106B (TMEM106B Human)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N-terminus and C-terminus are deleted. BAX(3-171)…
    Promoter
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228939-plasmid-map-sequence-id-469416

    Enlarge

  16. pDEST-Myc-FBW7 Delta WD40 (-2 to 7)

    Plasmid
    #197454
    Purpose
    The plasmid expresses delta WD40 deleted version of Myc-tagged FBW7 human isoform 1. WD40 propellars 2 to 7 are deleted. Used for mammalian overexpression and detection by western blotting.
    Depositor
    Omar Khan
    Article
    Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:
    Insert
    FBW7 Delta-WD40 propeller (FBXW7 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    FBW& dimerization domain deleted.
    Promoter
    CMV
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    197454-plasmid-map-sequence-id-394916

    Enlarge

  17. pDEST-Myc-FBW7 WD40 (All deleted)

    Plasmid
    #197455
    Purpose
    The plasmid expresses delta WD40 deleted version of Myc-tagged FBW7 human isoform 1. WD40 All are deleted. Used for mammalian overexpression and detection by western blotting.
    Depositor
    Omar Khan
    Article
    Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:
    Insert
    FBW7 (FBXW7 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    FBW& dimerization domain deleted.
    Promoter
    CMV
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    197455-plasmid-map-sequence-id-394905

    Enlarge

  19. pcDNA3-sPDGFRalpha-Fc-Y206S

    Plasmid
    #136439
    Purpose
    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc (Y206S mutant)
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    PDGFRalpha (PDGFRA Human)
    Use
    Tags
    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…
    Expression
    Mammalian
    Mutation
    Extracellular domain of PDGFR-alpha (a.a. 1-524) …
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136439-plasmid-map-sequence-id-271696

    Enlarge

  20. pcDNA3-sPDGFRalpha-Fc-V242K

    Plasmid
    #136440
    Purpose
    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc (V242K mutant)
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    PDGFRalpha (PDGFRA Human)
    Use
    Tags
    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…
    Expression
    Mammalian
    Mutation
    Extracellular domain of PDGFR-alpha (a.a. 1-524) …
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136440-plasmid-map-sequence-id-271678

    Enlarge

  21. AiP15385: pAAV-AiE0873m_3xC2-minBG-mScarlet-WPRE3-BGHpA (Alias: CN5385)

    Plasmid
    #230875
    Purpose
    AiE0873m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal cholinergic interneurons
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.
    Insert
    mScarlet
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    230875-plasmid-map-sequence-id-459525

    Enlarge

  22. AiP15747: pAAV-AiE0779m_3xC2-minBG-mScarlet-WPRE3-BGHpA (Alias: CN5747)

    Plasmid
    #230882
    Purpose
    AiE0779m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal direct pathway MSNs (D1-MSNs)
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.
    Insert
    mScarlet
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    230882-plasmid-map-sequence-id-459397

    Enlarge

  23. AiP15745: pAAV-AiE0452h-minBG-mScarlet-WPRE3-BGHpA (Alias: CN5745)

    Plasmid
    #230880
    Purpose
    AiE0452h is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal indirect pathway MSNs (D2-MSNs)
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.
    Insert
    mScarlet
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    230880-plasmid-map-sequence-id-469440

    Enlarge

  24. AiP15649: pAAV-AiE0780m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN5649)

    Plasmid
    #230879
    Purpose
    AiE0780m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal direct pathway MSNs (D1-MSNs)
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    230879-plasmid-map-sequence-id-459527

    Enlarge

  25. AiP13985: pAAV-AiE0445h_3xC2-minBG-jRGECO1a-WPRE3-BGHpA (Alias: CN3985)

    Plasmid
    #230865
    Purpose
    AiE0445h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal indirect pathway MSNs (D2-MSNs)
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.
    Insert
    jRGECO1a
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    230865-plasmid-map-sequence-id-459524

    Enlarge

  27. AiP15646: pAAV-AiE1426m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN5646)

    Plasmid
    #230884
    Purpose
    AiE1426m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical and striatal Sst-Chodl neurons
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    230884-plasmid-map-sequence-id-459521

    Enlarge

  28. AiP14530: pAAV-AiE0743m_3xC2-minBG-mTFP1-WPRE3-BGHpA (Alias: CN4530)

    Plasmid
    #230869
    Purpose
    AiE0743m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal cholinergic interneurons
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.
    Insert
    mTFP1
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    230869-plasmid-map-sequence-id-459488

    Enlarge

  29. AiP12909: pAAV-AiE0445h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2909)

    Plasmid
    #230862
    Purpose
    AiE0445h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal indirect pathway MSNs (D2-MSNs)
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    230862-plasmid-map-sequence-id-459486

    Enlarge

  30. AiP15100: pAAV-AiE0140h_3xC2-minBG-FlpO-4X2C-WPRE3-BGHpA (Alias: CN5100)

    Plasmid
    #230873
    Purpose
    AiE0140h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical and striatal Pvalb interneurons
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.
    Insert
    FlpO
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    230873-plasmid-map-sequence-id-459485

    Enlarge

  31. AiP14646: pAAV-AiE0779m_6xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4646)

    Plasmid
    #230872
    Purpose
    AiE0779m_6xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal direct pathway MSNs (D1-MSNs)
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.
    Insert
    jGCaMP8s
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    230872-plasmid-map-sequence-id-459483

    Enlarge

  35. AiP14133: pAAV-AiE0445h_3xC2-minBG-jGCaMP8m-WPRE3-BGHpA (Alias: CN4133)

    Plasmid
    #230867
    Purpose
    AiE0445h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal indirect pathway MSNs (D2-MSNs)
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.
    Insert
    jGCaMP8m
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    230867-plasmid-map-sequence-id-459475

    Enlarge

  36. AiP14034: pAAV-AiE0445h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4034)

    Plasmid
    #230866
    Purpose
    AiE0445h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal indirect pathway MSNs (D2-MSNs)
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.
    Insert
    CoChR-EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    230866-plasmid-map-sequence-id-459328

    Enlarge

  37. AiP15481: pAAV-AiE0140h_3xC2-minBG-iCre(R297T)-4X2C-BGHpA (Alias: CN5481)

    Plasmid
    #230876
    Purpose
    AiE0140h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Pvalb interneurons
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.
    Insert
    iCre(R297T)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    230876-plasmid-map-sequence-id-459320

    Enlarge

  38. AiP15582: pAAV-AiE0780m-minBG-jRGECO1a-WPRE3-BGHpA (Alias: CN5582)

    Plasmid
    #230878
    Purpose
    AiE0780m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal direct pathway MSNs (D1-MSNs)
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.
    Insert
    jRGECO1a
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    230878-plasmid-map-sequence-id-459324

    Enlarge

  39. AiP15746: pAAV-AiE0780m-minBG-mScarlet-WPRE3-BGHpA (Alias: CN5746)

    Plasmid
    #230881
    Purpose
    AiE0780m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal direct pathway MSNs (D1-MSNs)
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.
    Insert
    mScarlet
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    230881-plasmid-map-sequence-id-459399

    Enlarge

Showing: 4201 - 4240 of 84526 results