165,744 results

1. LAMP1-mGFP

   Plasmid

   #34831

   Depositor

   Esteban Dell'Angelica

   Article

   Falcon-Perez et al J Cell Sci. 2005 Nov 15;118(Pt 22):5243-

   Insert

   LAMP1 (LAMP1 Human)
   

   Tags

   GSTGSTGSTGA linker, Kozak, and mGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAV1K-T5-gfp

   Plasmid

   #26702

   Depositor

   Ichiro Matsumura

   Article

   Bryksin et al PLoS One. 2010 . 5(10):e13244.

   Insert

   T5lacOlacOeGFP biobrick
   

   Expression

   Bacterial

   Available Since

   Nov. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-CMV-eGFP-hTau P301L-WPRE

   Plasmid

   #233043

   Purpose

   To Express a GFP human Tau P301L fusion protein from a CMV promoter

   Depositor

   Jonathan Ploski

   Insert

   eGFP-hTau P301L fusion (MAPT Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   EGFP

   Available Since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-CBLB

   Plasmid

   #192082

   Purpose

   Mammalian expression of human CBLB with FLAG tag

   Depositor

   Patrick Concannon

   Article

   Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

   Insert

   CBLB (CBLB Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-PIK3CA-E545K

   Plasmid

   #116485

   Purpose

   Lentiviral expression of PIK3CA E545K

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Lentiviral

   Mutation

   E545K

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDS220

   Plasmid

   #34980

   Purpose

   Expresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   ePDZb–mCherry
   

   Expression

   Yeast

   Promoter

   TEF

   Available Since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJBL7056

   Plasmid

   #167225

   Purpose

   orthogonal T7 RNAP sfGFP reporter

   Depositor

   Julius Lucks

   Article

   Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

   Insert

   pAKSIRV-sfGFP
   

   Use

   Synthetic Biology

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. GLP1R-DuET

   Plasmid

   #213253

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GLP1R (GLP1R Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

   Plasmid

   #208942

   Purpose

   The CE1 construct, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJBL7085

    Plasmid

    #167249

    Purpose

    orthogonal mRFP reporter output for cell-free expression

    Depositor

    Julius Lucks

    Article

    Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

    Insert

    pAKSIRV-mRFP1
    

    Use

    Synthetic Biology

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.CAG.(cyto).cpSFGFP.HaloTag

    Plasmid

    #209654

    Purpose

    Non-responsive control

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    cpSFGFP.HaloTag
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiCRISPRv2 hygro

    Plasmid

    #98291

    Purpose

    Variant of lentiCRISPRv2 that confers hygromycin resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Promoter

    EF-1a

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-EF1A-CD19.28H.28TM.28.3z

    Plasmid

    #200678

    Purpose

    Modular CD28hinge-CD28transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD28HD-CD28TM-CD28-CD3z-P2A-EGFP (CD28, CD247 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Promoter

    EF1a-short

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHrD-IRES-Fluc

    Plasmid

    #194250

    Purpose

    Human RNAP1 promoter region driving firefly luciferase expression from IRES. Constructed by Ghoshal, et al. J Biol Chem (2004). Please cite this original manuscript!

    Depositor

    Samson Jacob

    Article

    McCool et al RNA. 2022 Nov 2. pii: rna.079149.122. do

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Promoter

    human rDNA promoter and beginning of 5' ETS …

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. RNA motif plasmid cloning backbone

    Plasmid

    #107253

    Purpose

    RNA motif plasmid with BoxB sites. Digest with BsmBI and insert RNA motif of interest. Stuffer size 1.8kb

    Depositor

    Paul Khavari

    Article

    Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

    Insert

    RNA motif plasmid cloning backbone
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTYB1-eIF4A

    Plasmid

    #37225

    Depositor

    Jon Lorsch

    Article

    Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

    Insert

    eIF4A
    

    Tags

    intein

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pJBL7079

    Plasmid

    #167244

    Purpose

    cell-free lac sensor with GFP output

    Depositor

    Julius Lucks

    Article

    Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

    Insert

    pLac-sfGFP
    

    Use

    Synthetic Biology

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJBL7080

    Plasmid

    #167245

    Purpose

    cell-free lac sensor with RFP output

    Depositor

    Julius Lucks

    Article

    Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

    Insert

    pLac-mRFP1
    

    Use

    Synthetic Biology

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJBL7081

    Plasmid

    #167246

    Purpose

    cell-free lac sensor with Mango output

    Depositor

    Julius Lucks

    Article

    Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

    Insert

    pLac-Mango
    

    Use

    Synthetic Biology

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJBL7082

    Plasmid

    #167247

    Purpose

    cell-free lac sensor with XylE output

    Depositor

    Julius Lucks

    Article

    Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

    Insert

    pLac-XylE
    

    Use

    Synthetic Biology

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pJBL7086

    Plasmid

    #167250

    Purpose

    orthogonal Mango reporter output for cell-free expression

    Depositor

    Julius Lucks

    Article

    Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

    Insert

    pAKSIRV-Mango
    

    Use

    Synthetic Biology

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pR6K-GFP-CASTIF

    Plasmid

    #199653

    Purpose

    R6K plasmid encoding a type I-F CAST but no CRISPR array

    Depositor

    IIya Finkelstein

    Insert

    CAST I-F systems
    

    Expression

    Bacterial

    Promoter

    J23119 promoter

    Available Since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pClodANL-FGF2

    Plasmid

    #107871

    Purpose

    Bacterial expression of FGF2-NanoLuc

    Depositor

    Sanford Simon

    Article

    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

    Insert

    FGF2
    

    Use

    Luciferase

    Tags

    NanoLuc

    Expression

    Bacterial

    Mutation

    wt sequence

    Promoter

    pro D

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCMV-OptoFGFR1

    Plasmid

    #59776

    Purpose

    Expresses myristoylation signal peptide-FGFR1 cytoplasmic region-PHR domain of cryptochrome2-mCitrine fused protein

    Depositor

    Won Do Heo

    Article

    Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi

    Insert

    OptoFGFR1 (FGFR1 Human, Mustard Weed)
    

    Tags

    mCitrine

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. pGL4-ERSE1-luc2P-Hygro

    Plasmid

    #101789

    Purpose

    Luferase reporter plasmid containing three tandem repeats of the ER stress response element-I (ERSE-I)

    Depositor

    Seiichi Oyadomari

    Article

    Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

    Insert

    3xERSE-I
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Promoter

    minimal TATA-box promoter with low basal activity

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. phH1-gRNA

    Plasmid

    #53186

    Purpose

    Expresses the S. pyogenes sgRNA from the H1 promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    human H1

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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27. pMV306G13+Lux

    Plasmid

    #26160

    Depositor

    Siouxsie Wiles, Brian Robertson

    Article

    Andreu et al PLoS One. 2010 May 24;5(5):e10777.

    Insert

    G13 promoter + Bacterial luciferase operon
    

    Expression

    Bacterial

    Mutation

    It contains a gram-positive enhanced translation …

    Available Since

    Feb. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCDNA3-Myc3-Nrf2

    Plasmid

    #21555

    Purpose

    Mammalian expression of human Nrf2 with Myc tag

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

    Insert

    nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)
    

    Tags

    myc3

    Expression

    Mammalian

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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29. PB-CAG-mRuby3-Gal8-P2A-Zeo

    Plasmid

    #150815

    Purpose

    Mammalian expression of mRuby3-Galectin8 (Gal8) fusion protein

    Depositor

    Jordan Green

    Article

    Rui et al Sci Adv. 2022 Jan 7;8(1):eabk2855. doi:

    Insert

    mRuby3-Gal8-P2A-Zeo (LGALS8 Human, Synthetic)
    

    Tags

    Gal8 is fused to the c-terminus of mRuby3

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCAS9-mCherry-Frame +2

    Plasmid

    #66941

    Purpose

    CRISPaint frame selector +2

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA frame +2
    

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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