161,641 results

1. pJQ200SK

   Plasmid

   #78497

   Purpose

   Suicide vector for gene replacement

   Depositor

   Michael Hynes

   Article

   Quandt et al Gene. 1993 May 15;127(1):15-21.

   Type

   Empty backbone

   Use

   Suicide plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2lox-cAMPr

   Plasmid

   #99143

   Purpose

   Expresses cAMPr in mammalian cells

   Depositor

   Justin Blau

   Article

   Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

   Insert

   cAMPr
   

   Use

   Cre/Lox

   Tags

   cpGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. nls-EGFP

   Plasmid

   #67652

   Purpose

   Mammalian expression vector driving nls-EGFP.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   nls-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. p38-KTR-mStrawberry reporter

   Plasmid

   #158687

   Purpose

   This plasmid is a p38 pathway reporter. It has CMV promoter driving the expression of p38-KTR fused to mStrawberry-pGK-BSD

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   mStrawberry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-6xGLY-His-tag-kanMX6

   Plasmid

   #20763

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. 35S-VP16-GWY/pAM

   Plasmid

   #201234

   Purpose

   Used to produce and express in planta C-terminal fusion proteins with the VP16 activation domain

   Depositor

   Michael Ayliffe

   Article

   Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

   Insert

   35S-VP16-GWY
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT3TS-RfxCas13d-HA

   Plasmid

   #141320

   Purpose

   Plasmid to carry out IVT of RfxCas13d (human codon-optimized)

   Depositor

   Miguel Angel Moreno-Mateos, Ariel Bazzini

   Article

   Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

   Insert

   Rfx-Cas13d
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEZYflag

   Plasmid

   #18700

   Purpose

   pFlag-CMV-2 converted Gateway Destination vector for expressing Flag tagged fusion proteins in mammalian cells

   Depositor

   Yu-Zhu Zhang

   Article

   Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

   Type

   Empty backbone

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. lenti_dCas9-KRAB-MeCP2

   Plasmid

   #122205

   Purpose

   Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.

   Depositor

   Andrea Califano

   Article

   Tan et al bioRxiv 2021.06.28.449297

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   Lentiviral

   Tags

   KRAB-MeCP2

   Expression

   Mammalian

   Mutation

   Cas9 (D10A, H840A)

   Promoter

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-SMVP-Cas9N

    Plasmid

    #80930

    Purpose

    Expresses Cas9N in mammalian cells.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-N-Flag-NLRP3

    Plasmid

    #75127

    Purpose

    Expresses N-terminal Flag-tagged NLRP3 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-2xFYVE

    Plasmid

    #140047

    Purpose

    Expresses GFP-tagged HRS 2xFYVE in mammalian cells

    Depositor

    Harald Stenmark

    Article

    Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:

    Insert

    tandem HRS FYVE domain (Hgs Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    two FYVE domains (amino acids 147-223), linked by…

    Promoter

    CMV

    Available since

    May 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-PE2-P2A-GFP

    Plasmid

    #132776

    Purpose

    Prime editing in mammalian cells with co-translational GFP expression

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    PE2-P2A-GFP
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. 35S-GAL4BD-GWY/pAM

    Plasmid

    #201233

    Purpose

    Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD

    Depositor

    Michael Ayliffe

    Article

    Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

    Insert

    35S-GAL4BD-GWY
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX_DSB_Spectrum-V1

    Plasmid

    #192474

    Purpose

    Lentiviral DSB Spectrum V1 Reporter System

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    BFP1.2, incompleteGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Dnmt1-PE3-loxP

    Plasmid

    #207862

    Purpose

    AAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNA

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    NpuC-Cterm-PEmaxDRNaseH-dualU6-Dnmt1-PE3-loxP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See Manuscript

    Promoter

    Cbh

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. CAHS2 (pBS0249)

    Plasmid

    #185139

    Purpose

    For the mammalian expression of the tardigrade protein CAHS2. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    CAHS2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. DNMT3A-dCas9

    Plasmid

    #100090

    Purpose

    DNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    DNMT3A (DNMT3A Human)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-DNMT3A-dCas9-NLS

    Expression

    Mammalian

    Mutation

    includes aa 602-912

    Promoter

    CMV

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-Bxb1-His

    Plasmid

    #123132

    Purpose

    Bacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Bxb1 integrase
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCh-Climp63

    Plasmid

    #136293

    Purpose

    expression of Climp63

    Depositor

    Gia Voeltz

    Article

    Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

    Insert

    Cytoskeletal-linking protein 63 (Ckap4 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. lentiCRISPRv2_AMBRA1#2

    Plasmid

    #174153

    Purpose

    Lentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 gene

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA (AMBRA1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCS2-nCas9n

    Plasmid

    #47929

    Purpose

    expression of an optimized Cas9 for genome-editing in zebrafish

    Depositor

    Wenbiao Chen

    Article

    Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

    Insert

    nls-zcas9-nls
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-6XHis-human SUMO 2

    Plasmid

    #133771

    Purpose

    express 6Xhis- SUMO 2 protein

    Depositor

    Martine Roussel

    Article

    Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

    Insert

    SUMO 2 (SUMO2 Human)
    

    Use

    Tags

    6XHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. LAMP1-APEX2

    Plasmid

    #215555

    Purpose

    Stable, inducible expression in lysosome

    Depositor

    Nevan Krogan

    Article

    Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

    Insert

    LAMP1-EGFP-Flag tag-APEX2-NES
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEGFP-Akt1(WT)

    Plasmid

    #86637

    Purpose

    Expresses eGFP-tagged human Akt1(WT)

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. pSIRV-AP-1-mCherry

    Plasmid

    #118095

    Purpose

    Fluorescent reporter for AP-1 activation

    Depositor

    Peter Steinberger

    Article

    Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

    Insert

    mCherry
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    minimal promoter with AP-1 responsive element

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pDONR223_KRAS_p.G12V

    Plasmid

    #81665

    Purpose

    Gateway Donor vector containing KRAS,part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    G12V

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTALdNC-AAVS1_T1

    Plasmid

    #80495

    Purpose

    AAVS1 TALEN expression vector (Left)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    AAVS1 TALEN (Left)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTALdNC-AAVS1_T2

    Plasmid

    #80496

    Purpose

    AAVS1 TALEN expression vector (Right)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    AAVS1 TALEN (Right)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLV-EF1A-CD19.28H.28TM.28.3z

    Plasmid

    #200678

    Purpose

    Modular CD28hinge-CD28transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD28HD-CD28TM-CD28-CD3z-P2A-EGFP (CD28, CD247 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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