We narrowed to 7,502 results for: RAP

Showing: 3211 - 3240 of 7502 results

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pGal4AD-CIBN

Plasmid

#28246

Purpose

Expresses Gal4 Activation Domain fused to CIBN (aa1-170) for blue-light-induced transcription activation

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CIB1 (1-171aa)

Tags

Gal4AD

Expression

Yeast

Available Since

March 23, 2011

Availability

Academic Institutions and Nonprofits only

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MTK4_001

Plasmid

#123810

Purpose

Encodes Bovine Growth Hormone polyadenylation signal as a Type 4 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Bgh pA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pGL-p27UTR

Plasmid

#20881

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

p27 3'UTR (Cdkn1b Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
pTP341 pHAGE2 CMV TagBFP-3xFLAG

Plasmid

#199391

Purpose

Lentiviral transfer plasmid for constitutive expression of a TagBFP-3xFLAG in mammalian cells

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

TagBFP-3xFLAG

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

Plasmid

#222993

Purpose

Mammalian expression of iGeoCas9(C1) construct with EGFP-targeting sgRNA

Depositor

Jennifer Doudna

Article

Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

Insert

HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

Use

CRISPR

Tags

Puro

Expression

Mammalian

Promoter

pCAG

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pH6HTC_IRP1-Halo

Plasmid

#175305

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

IRP1 (ACO1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pGL2399

Plasmid

#137051

Purpose

Expression of rapamycin-inducible dimerisation of split Cre from ribosomal locus in Leishmania parasite; pRIB-DiCre

Depositor

Jeremy Mottram

Article

Duncan et al Methods Mol Biol. 2019;1971:211-224. doi

Inserts

Split Cre Recombinase Rapamycin Inducible Dimerisation
Blasticidin-S Deaminase

Use

Cre/Lox; Leishmania ribosomal locus expression

Promoter

For integration into the 18S ribosomal locus

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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SB_loop

Plasmid

#110103

Purpose

pBXPHM3 with scaffold Sybody of the loop library

Depositor

Markus Seeger

Article

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Insert

scaffold Sybody of the loop library

Tags

His

Expression

Bacterial

Promoter

PBAD

Available Since

May 24, 2018

Availability

Academic Institutions and Nonprofits only

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Sirius-mT-Sapphire_pcDNA3

Plasmid

#89584

Purpose

Genetically encoded ratiometric fluorescent thermometer

Depositor

Takeharu Nagai

Article

Nakano et al PLoS One. 2017 Feb 17;12(2):e0172344. do

Insert

Sirius-mT-Sapphire

Expression

Mammalian

Promoter

CMV

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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Ii-Str_ManII-SBP-EGFP

Plasmid

#65255

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ii-Streptavidin and truncated Mannosidase II

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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Omega Destination-CMV::ELuc:bGH

Plasmid

#124528

Purpose

Omega destination vector, that has a CMV:ELUC:bgH in the backbone

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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YF-RhoA(CA)

Plasmid

#20153

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP-RhoA(CA) (RHOA Human)

Expression

Mammalian

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pOZ-N-FH MERIT40

Plasmid

#27498

Depositor

Roger Greenberg

Article

Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

Insert

MERIT40 (BABAM1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
MTK1_022

Plasmid

#123684

Purpose

Encodes ConLS and the human A4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector LS-A4 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Ii-Str_ManII-SBP-mCherry

Plasmid

#65256

Purpose

synchronize trafficking of ManII from the ER

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ii-Streptavidin and truncated Mannosidase II

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1 RagC shRNA #1

Plasmid

#26629

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RagC shRNA (RRAGC Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
MTK1_024

Plasmid

#123686

Purpose

Encodes ConL2 and human C3 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L2-C3 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pSin_EF1a_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR

Plasmid

#176310

Purpose

Expresses CreN59-nMag-NLS with mCherry marker.

Depositor

Yingxiao Wang

Article

Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

Insert

CreN59_nMag_NLS_P2A_mCherry_IRES_puroR

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

human EF1alpha

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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YF-Rac1(CA)

Plasmid

#20150

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP-Rac1(CA) (RAC1 Human)

Expression

Mammalian

Mutation

constitutively active mutation

Available Since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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MTK1_023

Plasmid

#123685

Purpose

Encodes ConL1 and human B1 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L1- B1 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pOZ-N-FH KIAA0157

Plasmid

#27499

Depositor

Roger Greenberg

Article

Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

Insert

KIAA0157 (ABRAXAS2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 1, 2011

Availability

Academic Institutions and Nonprofits only
pJBL711

Plasmid

#140381

Purpose

Expresses OtrR protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-OtrR-T

Tags

TEV-6XHis

Expression

Bacterial

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagA 21L

Plasmid

#19299

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding A (RRAGA Human)

Tags

GST and HA

Expression

Mammalian

Mutation

T21L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pCDH_SBP-EGFP-Ecadherin

Plasmid

#65290

Purpose

Ecadherin reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Use

Lentiviral

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pMUT2-pTcpP-mCherry

Plasmid

#192861

Purpose

pMUT2 based vector for sensing human primary bile acids in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

TcpP-CadC fusion protein and cognate promoter inducible by human primary bile acids

Expression

Bacterial

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CD4-sTb(C)-M13-GFP

Plasmid

#219780

Purpose

sTb(C) half CaST for HEK cell expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CD4-sTb(C)-M13-GFP

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1

Plasmid

#222992

Purpose

Mammalian expression of iNme2Cas9 construct with EGFP-targeting sgRNA

Depositor

Jennifer Doudna

Article

Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

Insert

HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1

Use

CRISPR

Tags

Puro

Expression

Mammalian

Promoter

pCAG

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5 HA3-eDHFR-T2A-puro

Plasmid

#86395

Purpose

for C-terminal tagging with eDHFR trimethoprim regulated degron with puro selection

Depositor

David Bentley

Article

Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

Insert

HA3 eDHFR T2A puro

Use

CRISPR

Tags

HA3 eDHFR T2A puro

Mutation

R12Y, G67S and Y100I mutations in E coli DHFR

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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EW410 IKZF1tar x8-H2B-GFP

Plasmid

#236156

Purpose

Fuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with eight IKZF1 binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

E1b minimal promoter with eight IKZF1 binding sit…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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MTK234_008

Plasmid

#123902

Purpose

Encodes the GFP dropout cassette for multi-TU destination vectors as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

GFP dropout for multiTU destination vectors

Expression

Mammalian

Available Since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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