We narrowed to 11,959 results for: pcDNA

1. pcDNA-NBid-PhoCl2c-CBid

   Plasmid

   #164051

   Purpose

   Optogenetic manipulation of cell apoptosis using PhoCl2c

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   NBid-PhoCl2c-CBid
   

   Use

   Tags

   kozak sequence

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promotor

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-Akt-STOPS-NLS

   Plasmid

   #175007

   Purpose

   Nuclear-targeted Akt-STOPS.

   Depositor

   Jin Zhang

   Article

   Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

   Insert

   mCherry-Akt-STOPS-NLS
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA5-FRT/TO-N-mEGFP-PARP7

   Plasmid

   #178004

   Purpose

   Expresses N-terminally mEGFP-tagged PARP7

   Depositor

   Karla Feijs

   Article

   Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

   Insert

   PARP7 (TIPARP Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-FLAG-mTET2 CD mutant

   Plasmid

   #89896

   Purpose

   Expresses mouse TET2 CD mutant in mammalian cells

   Depositor

   Yue Xiong

   Article

   Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

   Insert

   TET2 (Tet2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   CD (cysteine-rich, dioxygenase domain) truncation…

   Promoter

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA 3.1 AURKA Lys162Met

   Plasmid

   #157751

   Purpose

   Expression of kinase-dead AURKA K62M in mammalian cells

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   AURKA K162M is a kinase-dead version of AURKA

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5/FRT/TO GFP DNAJB6b

   Plasmid

   #19501

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DNAJB6b (DNAJB6 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-FLAG-Dicer-delHelicC

   Plasmid

   #51366

   Depositor

   Narry Kim

   Article

   Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

   Insert

   Dicer (DICER1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   From N terminus to HelicC domain deletion

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LARG mCherry sspB in pcDNA3.1

   Plasmid

   #90459

   Purpose

   RhoA mediated cell migration

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

   Inserts

   LARG DH-PH (aa 766–1138) (ARHGEF12 Human)
   mCherry
   sspB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R73Q

   Promoter

   CMV

   Available since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-MS2-HA-Hs4E-T_AA

   Plasmid

   #148233

   Purpose

   Mammalian Expression of Hs4E-T

   Depositor

   Elisa Izaurralde

   Insert

   Hs4E-T (EIF4ENIF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5/FRT/TO-GAlpha15-RLuc8

    Plasmid

    #140984

    Purpose

    Encodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlpha15-RLuc8 (GNA15 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-mCherry-CyrCR1.mutant

    Plasmid

    #128746

    Purpose

    Expresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Cyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5 point mutations disrupting the miR-7 site

    Promoter

    CMV

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDNA3-Flag MKK6(K82A)

    Plasmid

    #13519

    Depositor

    Roger Davis

    Article

    Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

    Insert

    MKK6(K82A) (MAP2K6 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Lys-82Ala

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5/FRT/TO GFP DNAJB6a

    Plasmid

    #19502

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB6a (DNAJB6 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-ArchT-BFP2-TSERex

    Plasmid

    #123312

    Purpose

    Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

    Depositor

    Yulong Li

    Article

    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

    Insert

    ArchT-BFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5 FRT TO-EGFP-G3BP1-WT

    Plasmid

    #136003

    Purpose

    DOX-inducible WT G3BP1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-N-Myc_1-560 USP7

    Plasmid

    #131244

    Purpose

    Mammalian expression of N-terminally Myc-tagged USP7, minus the UBL domains

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Expresses USP7 amino acids 1 - 560. This truncati…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5-FRT-TOgw-C-mCherry

    Plasmid

    #223952

    Purpose

    Gateway cloning of genes of interest with a C-terminal mCherry fusion; generation of stable cells with doxycycline inducible expression

    Depositor

    Roko Žaja, Karla Feijs

    Type

    Empty backbone

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1/NDUFS2/myc-His

    Plasmid

    #127486

    Purpose

    Expresses myc-tagged NDUFS2 in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 (NDUFS2 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5/FRT/TO-GAlphaoA-RLuc8

    Plasmid

    #140976

    Purpose

    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphaoA-RLuc8 (GNAO1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-FLAG-Dicer-delPAZ

    Plasmid

    #51367

    Depositor

    Narry Kim

    Article

    Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

    Insert

    Dicer (DICER1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    From N terminus to PAZ domain deletion

    Promoter

    CMV

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. FLAG-MBP1-E322TAG-W340A.pcDNA3-k

    Plasmid

    #127404

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site.

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: E322 to Amber codon and W34…

    Promoter

    CMV

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1 SARS-CoV-2 S D614

    Plasmid

    #158074

    Purpose

    Expresses SARS-CoV-2 spike protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-FLAG-Dicer-delDEAD

    Plasmid

    #51364

    Depositor

    Narry Kim

    Article

    Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

    Insert

    Dicer (DICER1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    From N terminus to DEAD domain deletion

    Promoter

    CMV

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-Y1175F-VEGFR2-mCherry

    Plasmid

    #139608

    Purpose

    Expresses a mutant (Y1175F) VEGFR2-mCherry fusion protein in mammalian cells

    Depositor

    Michael Welsh

    Article

    Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

    Insert

    KDR (KDR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Yellow Cameleon-Nano15/pcDNA3

    Plasmid

    #51961

    Purpose

    mammalian expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano15
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pcDNA-RanWT-mRFP1-polyA

    Plasmid

    #59750

    Purpose

    Express wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Ran-mRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA5/FRT/TO-GAlphai3-RLuc8

    Plasmid

    #140975

    Purpose

    Encodes a G alpha subunit (GNAI3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphai3-RLuc8 (GNAI3 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1-FLAG-Dicer-delDUF

    Plasmid

    #51365

    Depositor

    Narry Kim

    Article

    Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

    Insert

    Dicer (DICER1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    From N terminus to DUF domain deletion

    Promoter

    CMV

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1/NDUFS7/myc-His

    Plasmid

    #127488

    Purpose

    Expresses myc-tagged NDUFS7 in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS7 (NDUFS7 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1-Flag-PGC-1alpha1

    Plasmid

    #45501

    Depositor

    Bruce Spiegelman, Jorge Ruas

    Article

    Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

    Insert

    PGC-1alpha Isoform1 (Ppargc1a Mouse)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Q339K relative to NM_008904

    Promoter

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3 Flag TSC2 (S939A)

    Plasmid

    #14132

    Depositor

    Brendan Manning

    Article

    Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

    Insert

    TSC2 S939A (TSC2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S939A.

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

32. 1478 pcDNA3 flag HA 14-3-3 beta

    Plasmid

    #8999

    Depositor

    William Sellers

    Insert

    14-3-3 beta (YWHAB Human)
    

    Use

    Tags

    HA and flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

33. pcDNA3.1his p66shc S36A (corrected)

    Plasmid

    #32575

    Depositor

    Toren Finkel

    Article

    Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

    Insert

    p66shc (SHC1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    S36A

    Promoter

    CMV

    Available since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1-Cerulean-Epac2-Venus

    Plasmid

    #138196

    Purpose

    FRET-based biosensor for monitoring Epac2 activation.

    Depositor

    Jin Zhang

    Article

    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

    Insert

    Cerulean-Epac2-Venus (Rapgef4 Mouse, Synthetic)
    

    Use

    Tags

    Cerulean and Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCDNA3-AUP1-FLAG-DATDCUE

    Plasmid

    #185340

    Purpose

    Assessing domain requirement sof AUP1

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Use

    Tags

    FLAG, 6xHis

    Expression

    Mammalian

    Mutation

    deletion of aa 90-337 corresponding to the acyl t…

    Promoter

    Available since

    May 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3puro-mGFP-FXR1a-sh5Resistant

    Plasmid

    #225448

    Purpose

    Express mEGFP-fusion protein of FXR1 isoform a with shRNA5-resistant silent mutation

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    FXR1 isoform a (FXR1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-SEP-Glur2 (MCA10)

    Plasmid

    #64941

    Purpose

    mammalian expression of rat GluR2

    Depositor

    Jeremy Henley

    Article

    Ashby et al J Neurosci. 2004 Jun 2;24(22):5172-6.

    Insert

    GluR2 (Gria2 Rat)
    

    Use

    Tags

    SEP (superecliptic pHluorin)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1-SARS-CoV-2-S (D614G)

    Plasmid

    #215838

    Purpose

    Expresses SARS-CoV-2 Spike protein in mammalian cells

    Depositor

    Thomas Gallagher

    Article

    Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

    Insert

    SARS-CoV-2 Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1-Hygro(+)-PSTCD-cGAS

    Plasmid

    #102605

    Purpose

    Mammalian expression of cGAS fused to PSTCD tag

    Depositor

    Nicolas Manel

    Article

    Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

    Insert

    cGAS (CGAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.1+(rInsp)-Ins-GLuc

    Plasmid

    #89928

    Purpose

    Expresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.

    Depositor

    Melanie Cobb

    Article

    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E

    Insert

    human insulin-Gaussia-Luciferase (INS Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    DNA sequence for Gaussia luciferase was humanized…

    Promoter

    rat Insulin II promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge