We narrowed to 31,327 results for: uros

1. Cav2.2 BBS exon37a pCAGGS

   Plasmid

   #206086

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and alternative exon 37a

   Depositor

   Annette Dolphin

   Article

   Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Expression

   Mammalian

   Promoter

   CMV/B-actin

   Available Since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP Cav2.2 HA pcDNA3

   Plasmid

   #206053

   Purpose

   expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II

   Depositor

   Annette Dolphin

   Article

   Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-DIO-basic

   Plasmid

   #196882

   Purpose

   Used for preparation of double-floxed inverse ORF vectors

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Type

   Empty backbone

   Use

   Cre/Lox; Dre/rox

   Expression

   Mammalian

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBactin-mOrange2-CI

   Plasmid

   #196852

   Purpose

   Expression of red fluorescent reporter mOrange driven by the strong Beta-actin promoter

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mOrange2
   

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEY80

   Plasmid

   #191075

   Purpose

   flp-18(AVA = 4.2-1k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   flp-18(AVA = 4.2-1k) (flp-18 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEY73

   Plasmid

   #191070

   Purpose

   cho-1(3.7-1.7k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   cho-1(3.7-1.7k) (cho-1 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEY72

   Plasmid

   #191069

   Purpose

   cho-1(3.7k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   cho-1(3.7k) (cho-1 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-MNTS-1-p75

   Plasmid

   #182945

   Purpose

   To express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein that can bind only all 3 TRK receptors and not p75NTR

   Depositor

   Pantelis Tsoulfas

   Article

   Urfer et al EMBO J. 1994 Dec 15. 13(24):5896-909.

   Insert

   MNTS-1-p75 (NTF3, NT3 Human, Synthetic)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV promoter and enhancer

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-MNTS-1

   Plasmid

   #182944

   Purpose

   To express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein can bind all 3 TRK receptors and p75NTR

   Depositor

   Pantelis Tsoulfas

   Article

   Urfer et al EMBO J. 1994 Dec 15. 13(24):5896-909.

   Insert

   MNTS-1 (NTF3 Human, Synthetic, Homo sapiens)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   This is the Human NT-3 with mutations on the matu…

   Promoter

   CMV promoter and enhancer

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-CaMKII-GCaMP6f-4x128-mAGNET

    Plasmid

    #166023

    Purpose

    Expresses GCaMP6f in CaMKII+ cells with low miRNA-128 expression

    Depositor

    Xue Han

    Article

    Keaveney et al J Neurosci. 2020 Dec 9;40(50):9576-9588.

    Insert

    GCaMP6f with 4 miR-128 binding sites
    

    Use

    AAV and Mouse Targeting

    Tags

    None

    Expression

    Mammalian

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-CaMKII-mRuby2-4x128-mAGNET

    Plasmid

    #166021

    Purpose

    Expresses mRuby in CaMKII+ cells with low miRNA-128 expression

    Depositor

    Xue Han

    Article

    Keaveney et al J Neurosci. 2020 Dec 9;40(50):9576-9588.

    Insert

    mRuby2 with 4 miR-128 binding sites
    

    Use

    AAV and Mouse Targeting

    Tags

    None

    Expression

    Mammalian

    Mutation

    None

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPRIME-CMV-GFP-shNts2

    Plasmid

    #132714

    Purpose

    Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Nts

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    shNts(2) (Nts Rat)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPRIME-CMV-GFP-shNts1

    Plasmid

    #132713

    Purpose

    Encodes short hairpin RNA (shRNA) #1 that targets the the 3’-untranslated region of the rat Nts gene

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    shNts(1) (Nts Rat)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Pmyo-3::hpo-30 gDNA (pBAB204)

    Plasmid

    #117385

    Purpose

    Allows for body-wall muscle expression of HPO-30

    Depositor

    Kavita Babu

    Article

    Sharma et al J Neurosci. 2018 Aug 8;38(32):7072-7087.

    Insert

    genomic DNA of hpo-30 (833 bp)
    

    Expression

    Worm

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRII-Topo Frmd4b

    Plasmid

    #45867

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

    Insert

    Frmd4b in situ probe (Frmd4b Mouse)
    

    Use

    In situ

    Mutation

    contains bp# 3933-4535 of NM_145148.2

    Available Since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pCRII-Topo Lpl in situ probe

    Plasmid

    #45630

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

    Insert

    Lpl in situ probe (Lpl Mouse)
    

    Use

    In situ

    Mutation

    fragment contains bp# 3249-3900 of NM_008509.2

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pFSW Doc2alpha D291N mutant

    Plasmid

    #128813

    Purpose

    To make virus expressing Doc2alpha D291 mutant

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2alpha (Doc2a Rat)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Mutation

    D291N

    Promoter

    hSyn (human synapsin I promoter)

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFSW Doc2beta D303N mutant

    Plasmid

    #128815

    Purpose

    To make virus expressing Doc2beta D303N mutant

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2beta (Doc2b Rat)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Mutation

    D303N

    Promoter

    hSyn (human synapsin I promoter)

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFSW Doc2alpha Halotag

    Plasmid

    #128817

    Purpose

    To make virus expressing Doc2alpha Halotag

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2alpha (Doc2a Rat)
    

    Use

    Lentiviral

    Tags

    HaloTag-IRES-EGFP

    Promoter

    hSyn (human synapsin I promoter)

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFSW Doc2beta Halotag

    Plasmid

    #128818

    Purpose

    To make virus expressing Doc2beta Halotag

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2beta (Doc2b Rat)
    

    Use

    Lentiviral

    Tags

    HaloTag-IRES-EGFP

    Promoter

    hSyn (human synapsin I promoter)

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PB-TO-hNIL

    Plasmid

    #172113

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Promoter

    EF1a and TRE3G

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAX FLAG APP

    Plasmid

    #30154

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein (APP Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    wild type splice variant 751

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAX APP 751

    Plasmid

    #30138

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein (APP Human)
    

    Expression

    Mammalian

    Mutation

    wild type splice variant 751

    Available Since

    Aug. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST

    Plasmid

    #174007

    Purpose

    Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR under the control of an hSyn promoter. jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A.

    Depositor

    Mark Histed

    Article

    LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22

    Has Service

    AAV9

    Insert

    jGCaMP8s-P2A-ChrimsonR-ST
    

    Use

    AAV

    Tags

    6xHis and soma targeting motif

    Promoter

    hSyn

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG_PSD95.FingR-eGFP-CCR5TC

    Plasmid

    #46295

    Depositor

    Don Arnold

    Article

    Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

    Insert

    PSD95.FingR-eGFP-CCR5TC (DLG4 Human, Synthetic)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. lenti SYN-FLAG-dCas9-VPR

    Plasmid

    #114196

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the human SYN promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    SYN

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAX APP C99

    Plasmid

    #30146

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    APP C99 (APP Human)
    

    Expression

    Mammalian

    Mutation

    C-terminal from BACE site of APP

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAGGS-3xFLAG-bIV spectrin (repeats 13-15)

    Plasmid

    #213728

    Purpose

    Mammalian cell expression vector for overexpression of bIV spectrin (repeats 13-15) in NIH/3T3 cells.

    Depositor

    Chaolin Zhang

    Article

    Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

    Insert

    bIV spectrin (repeats 13-15) (Sptbn4 Mouse)
    

    Expression

    Mammalian

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pC13N-dCas9-BFP-KRAB

    Plasmid

    #127968

    Purpose

    constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    CLYBL-CAG-dCas9-NLS-BFP-KRAB
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET22b-T7-6h-GST-α-Synuclein

    Plasmid

    #225224

    Purpose

    Alpha synuclein gene fused with gst gene under t7 promoter for bacterial expression of alpha synuclein protein.

    Depositor

    Urartu Seker

    Article

    Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

    Inserts

    GST
    α-Synuclein
    

    Tags

    6xHis Tag and TEV Cleavage Site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Cx36-GCaMP

    Plasmid

    #123604

    Purpose

    Mouse Connexin 36-GCaMP3 fusion for measuring gap junction localized calcium signaling - expresses in mammalian cells

    Depositor

    John O'Brien

    Article

    Moore et al eNeuro. 2020 Mar 11. pii: ENEURO.0445-19

    Insert

    Connexin 36-GCaMP3 fusion (Gjd2 Mouse)
    

    Tags

    GCaMP3

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEGFP-TDP-43

    Plasmid

    #235487

    Purpose

    Expression of human TDP-43-EGFP

    Depositor

    Maxime Rousseaux

    Article

    Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

    Insert

    TARDBP (TARDBP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    April 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pCAX APP 695

    Plasmid

    #30137

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein (APP Human)
    

    Expression

    Mammalian

    Mutation

    wild type splice variant 695

    Available Since

    Aug. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCAX APP delta CT

    Plasmid

    #30143

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein (APP Human)
    

    Expression

    Mammalian

    Mutation

    deletion of C-terminal intracellular domain (afte…

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

35. phDLX1-N174

    Plasmid

    #60859

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses DLX1 under the EF1a promoter

    Depositor

    Andrew Yoo

    Article

    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

    Insert

    DLX1 (DLX1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5

    Plasmid

    #217510

    Purpose

    KD Recombinase dependent conditional smGdP-10XV5 cassette

    Depositor

    S. Lawrence Zipursky

    Article

    Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

    Insert

    KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5
    

    Tags

    smGdP-10XV5

    Expression

    Insect

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFlare9A-Plekha5E10 WT

    Plasmid

    #209576

    Purpose

    minigene construct for Plekha5 E10 alternative splicing

    Depositor

    Sika Zheng

    Article

    Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

    Insert

    Plekha5 (Plekha5 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-pCAG eGFP T2A SP HALO HAPLN1

    Plasmid

    #228200

    Purpose

    AAV vector expressing GFP and HaloTag-HAPLN1 under constitutive promoter

    Depositor

    Sungjin Park

    Article

    Sterin et al J Neurosci. 2024 Sep 9:e0666242024. doi:

    Insert

    HAPLN1 (Hapln1 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    constitutive CAG promoter

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAM-AAV-mSncg-Cre

    Plasmid

    #153207

    Purpose

    Mouse gamma-synuclein (mSncg) promoter-mediates Cre expression in retinal ganglion cells

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    mSncg

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PB-TO-NGN2-EMX1 BSD-mApple

    Plasmid

    #182311

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 and EMX1 expression, bsd selection, mApple

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Inserts

    hNGN2-P2A-EMX1 (NEUROG2, EMX1 Human)
    bsd-T2A-mycNLS-mApple
    

    Tags

    T2A-mycNLS-mApple

    Expression

    Mammalian

    Promoter

    EF1a and TRE3G

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pBactin-mNeonGreen-CI

    Plasmid

    #196850

    Purpose

    Expression of green fluorescent reporter mNeonGreen driven by the strong Beta-actin promoter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mNeonGreen
    

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. CMV-mEGFP-CREB (S133A)

    Plasmid

    #137002

    Purpose

    mEGFP-CREB donor for G-CREB with S133A mutation

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

    Insert

    CREB1 (Creb1 Rat)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Changes Serine 133 into Alanine

    Promoter

    CMV

    Available Since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCAX APP AENATA

    Plasmid

    #30144

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein (APP Human)
    

    Expression

    Mammalian

    Mutation

    YENPTY-->AENATA

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

44. HF-PX459 (V2)

    Plasmid

    #118632

    Purpose

    Plasmid encoding SpCas9-HF1, a single guide RNA and puromycin resistance

    Depositor

    Mike McGrew

    Article

    Idoko-Akoh et al Sci Rep. 2018 Oct 11;8(1):15126. doi: 10

    Insert

    hSpCas9-HF1-2A-Puro V2.0
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Asparagine 497 to Alanine, Arginine 661 to Alanin…

    Promoter

    Cbh

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pAAV-Ef1a-fDIO-rc[Chronos-eGFP]-WPRE

    Plasmid

    #203847

    Purpose

    Flp-dependent expression of Chronos fused to EGFP

    Depositor

    Ivo Spiegel

    Article

    Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

    Insert

    Chronos
    

    Use

    AAV

    Tags

    EGFP

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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46. pMT-C34-H

    Plasmid

    #223966

    Purpose

    Anti-MT-C34 heavy chain expression plasmid; to be used with the light chain plasmid, pMT-C34-L (Plasmid 223967), to make the antibody.

    Depositor

    Dmitriy Mazurov

    Article

    Kalinichenko et al Biology (Basel). 2024 Aug 29;13(9):675.

    Insert

    Variable domain of mouse anti-MT-C34 IgG1 heavy chain jointed to constant region of human IgG1 heavy chain
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. px551-mSncg-0.27kb-HA-SpCas9

    Plasmid

    #153208

    Purpose

    Truncated Mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates HA and Cas9 expression in retinal ganglion cells

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Insert

    Cas9
    

    Use

    AAV and CRISPR

    Tags

    HA

    Expression

    Mammalian

    Promoter

    Truncated mSncg (0.27kb)

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. pBactin-Tubg1-mEGFP

    Plasmid

    #196870

    Purpose

    Expression of the gamma-tubulin fused to enhanced (E)-GFP

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Tubg1-mEGFP (Tubg1 Mouse)
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCAG-lox-roxSTOProx-tRFP-pA-lox

    Plasmid

    #196888

    Purpose

    Dre-activated Cre-inactivated expression of turbo (t)RFP reporter.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    turbo (t)RFP
    

    Use

    Cre/Lox; Dre/rox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCAG-lox-roxSTOProx-lyn-mScarlet-pA-lox

    Plasmid

    #196889

    Purpose

    Dre-activated Cre-inactivated expression of lyn-mScarlet reporter.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    lyn-mScarlet
    

    Use

    Cre/Lox; Dre/rox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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