We narrowed to 9,259 results for: Coli

1. pLG007 (Ec86 retron)

   Plasmid

   #157885

   Purpose

   Expresses the Ec86 retron defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   Ec86 retron
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJM101(pUC18T-miniTn7T-gm-lacIq-Ptac)

   Plasmid

   #110558

   Purpose

   miniTn7 delivery plasmid with lacIq-Ptac inducible promoter

   Depositor

   Joanna Goldberg

   Article

   Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

   Insert

   lacIq-Ptac
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacIq-Ptac

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSF279

   Plasmid

   #167674

   Purpose

   Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. SupD v2

   Plasmid

   #198598

   Purpose

   Plasmid encodes two copies of the suppressor tRNA SupD that decodes amber (UAG) codons as serine.

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   SupD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLG013 (DRT type 5)

   Plasmid

   #157891

   Purpose

   Expresses the DRT type 5 defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   DRT type 5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. p201H Cas9

   Plasmid

   #59176

   Purpose

   Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Inserts

   Cas9
   hph
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   2x35S and StUbi-3P

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pScI_dCas9-CDA-UL

   Plasmid

   #108551

   Purpose

   Bacterial Target-AID UGI-LVA vector

   Depositor

   Akihiko Kondo

   Article

   Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

   Insert

   dCas9-PmCDA1-UGI-LVA
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   D10A and H840A for SpCas9

   Promoter

   lambda OR

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLG003 (Druantia type I)

   Plasmid

   #157881

   Purpose

   Expresses the Druantia type I defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   Druantia type I
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLG006 (Ec67 retron)

   Plasmid

   #157884

   Purpose

   Expresses the Ec67 retron defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   Ec67 retron
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLG008 (Ec78 retron)

    Plasmid

    #157886

    Purpose

    Expresses the Ec78 retron defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    Ec78 retron
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLG004 (RT-Abi-P2)

    Plasmid

    #157882

    Purpose

    Expresses the RT-Abi-P2 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    RT-Abi-P2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCold CL7-Cas12a

    Plasmid

    #124891

    Purpose

    obtaining full Cas12a RNP

    Depositor

    Yi Liu

    Article

    Qiao et al Commun Biol. 2019 May 3;2:161. doi: 10.1

    Insert

    Cas12a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLG025 (DUF4297 + HerA)

    Plasmid

    #157903

    Purpose

    Expresses the DUF4297 + HerA defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    DUF4297 + HerA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAJM.336

    Plasmid

    #108528

    Purpose

    LacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    LacIAM + PTac-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LacI Q18M A47V F161Y

    Promoter

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-EF1a-MinE-EGFP_IRES-Blast

    Plasmid

    #213113

    Purpose

    Expression of MinE-EGFP in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    MinE-EGFP (minE Escherichia coli)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 4A3.PBAD-repL(AT2).J23115-gfp

    Plasmid

    #197873

    Purpose

    Arabinose induced expression of repL(AT2) gene, which triggers DNA replication in trans, weak level of constitutive GFP expression

    Depositor

    Baojun Wang

    Article

    Huan et al Front Microbiol. 2023 Feb 9;14:1095671.

    Insert

    part kilA/repL(AT2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDR111_mannose_Bgal no NLS

    Plasmid

    #188399

    Purpose

    Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.

    Depositor

    Christopher Contag

    Article

    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

    Insert

    lacZ
    

    Use

    Tags

    PhoD secretion peptide

    Expression

    Bacterial

    Mutation

    Promoter

    D-mannose

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pScI_dCas9-CDA_J23119-sgRNA

    Plasmid

    #108550

    Purpose

    Bacterial Target-AID vector with sgRNA

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Inserts

    dCas9-PmCDA1
    Streptococcus pyogenes sgRNA
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    D10A and H840A for SpCas9

    Promoter

    J23119 and lambda OR

    Available since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLG032 (ppl)

    Plasmid

    #157910

    Purpose

    Expresses the ppl defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    ppl (PHP-ATPase)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pP3-GFP

    Plasmid

    #53436

    Purpose

    Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

    Depositor

    Cynthia Collins

    Article

    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

    Insert

    P3 promoter
    

    Use

    Synthetic Biology

    Tags

    none

    Expression

    Bacterial

    Mutation

    none

    Promoter

    P3

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTH847-gst-optsc

    Plasmid

    #96908

    Purpose

    Plasmid for yeast expression of GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    yeast codon optimised glutathione-S transferase
    

    Use

    Tags

    8xhistidine

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLG028 (hhe)

    Plasmid

    #157906

    Purpose

    Expresses the hhe defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    hhe (DUF4011-helicase-Vsr-DUF3320)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pT2ST

    Plasmid

    #59384

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-dfrA
    

    Use

    Template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAJM.661

    Plasmid

    #108532

    Purpose

    TtgRAM + PTtg-YFP Reporter. Nar sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    TtgRAM + PTtg-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    TtgR: R130H A144T A148V

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBbS8c-ddcpf1-Δ

    Plasmid

    #153039

    Purpose

    For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    Ascpf1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E993A

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28a-mCherryGGBsMTS

    Plasmid

    #133625

    Purpose

    Expression vector for mCherry fused to a membrane targeting sequence (MTS) of the B.subtilis MinD (C-terminal fusion) and an N-terminal His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

    Insert

    mCherryGGBsMTS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHC79

    Plasmid

    #167551

    Purpose

    Backbone plasmid to pPM1001, pPM1002, pPM1003, pPM2212 and pPM2213

    Depositor

    Renato Morona

    Article

    Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

    Type

    Empty backbone

    Use

    Cosmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRecE564T

    Plasmid

    #226825

    Purpose

    Expressing RecE*T controled by Ptrc promoter

    Depositor

    Sheng Yang

    Article

    Wang et al ACS Synth Biol. 2025 May 8. doi: 10.1021

    Insert

    recE*T
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAJM.690

    Plasmid

    #108533

    Purpose

    PcaUAM + P3B5B-YFP Reporter. DHBA sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PcaUAM + P3B5B-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    PcaU: K14N A165T N201H

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLG022 (dsr1)

    Plasmid

    #157900

    Purpose

    Expresses the dsr1 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    dsr1 (SIR2-DUF4020)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge