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Plasmid#200928Purposeexpresses MTS(S17A)-EGFP-V5 in mammalian cellsDepositorInsertEGFP
UseTagsV5 and mitochondrial targeting sequence (MTS)ExpressionMammalianMutationS17A in MTSPromoterCAGAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-MTS(S23A)-EGFP-V5
Plasmid#200929Purposeexpresses MTS(S23A)-EGFP-V5 in mammalian cellsDepositorInsertEGFP
UseTagsV5 and mitochondrial targeting sequence (MTS)ExpressionMammalianMutationMTS amino acid 23 mutated to AlaninePromoterCAGAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-MTS(S27A)-EGFP-V5
Plasmid#200930Purposeexpresses MTS(S27A)-EGFP-V5 in mammalian cellsDepositorInsertEGFP
UseTagsV5 and mitochondrial targeting sequence (MTS)ExpressionMammalianMutationS27A in MTSPromoterCAGAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-MTS(S31A)-EGFP-V5
Plasmid#200931Purposeexpresses MTS(S31A)-EGFP-V5 in mammalian cellsDepositorInsertEGFP
UseTagsV5 and mitochondrial targeting sequence (MTS)ExpressionMammalianMutationS31A in MTSPromoterCAGAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-MTS(S34A)-EGFP-V5
Plasmid#200932Purposeexpresses MTS(S34A)-EGFP-V5 in mammalian cellsDepositorInsertEGFP
UseTagsV5 and mitochondrial targeting sequence (MTS)ExpressionMammalianMutationS34A in MTSPromoterCAGAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS--MTS(S43A)-EGFP-V5
Plasmid#200933Purposeexpresses MTS(S43A)-EGFP-V5 in mammalian cellsDepositorInsertEGFP
UseTagsV5 and mitochondrial targeting sequence (MTS)ExpressionMammalianMutationS43A in MTSPromoterCAGAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-MTS(S6A)-EGFP-V5
Plasmid#200817Purposeexpresses MTS(S6A)-EGFP-V5 in mammalian cellsDepositorInsertEGFP
UseTagsV5 and mitochondrial targeting sequence (MTS)ExpressionMammalianMutationChanged Serine 6 to Alanine in MTSPromoterCAGAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-MTS(S12A)-EGFP-V5
Plasmid#200818Purposeexpresses MTS(S12A)-EGFP-V5 in mammalian cellsDepositorInsertEGFP
UseTagsV5 and mitochondrial targeting sequence (MTS)ExpressionMammalianMutationS12A in MTSPromoterCAGAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV-FOXA1-T2A-eGFP
Plasmid#182337PurposeExpresses human FOXA1 and eGFP via T2A linker under control of a CMV promoter.DepositorInsertForkhead Box A1 (FOXA1 Human)
UseTagsT2A-eGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
Venus-30aa-Noxa-4E-pEGFP-C1
Plasmid#177424PurposeTo express Venus fused to Noxa-4E, with a longer linker sequence between compared to Addgene #166745.DepositorInsertNoxa, PMA-induced protein 1 (PMAIP1 Human)
UseTagsVenusExpressionMammalianMutation4 hydrophobic residues in the BH3 region mutated …PromoterCMVAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_NES-Kinprola_on-mEGFP_WRPE-SV40
Plasmid#233368PurposehSyn1 driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through AAV transductionDepositorInsertNES-Kinprola_on-mEGFP
UseAAVTagsNES and mEGFPExpressionMutationPromoterhSyn1Available sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_NES-Kinprola_PKA_T/A-mEGFP_WRPE-SV40
Plasmid#233367PurposehSyn1 driven expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA fused to mEGFP for neuronal expression through AAV transductionDepositorInsertNES-Kinprola_PKA_T/A-mEGFP
UseAAVTagsNES and mEGFPExpressionMutationPromoterhSyn1Available sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_off-mEGFP
Plasmid#233356PurposeCMV driven expression of the inactive kinase activity recorder negative control Kinprola_off in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_off-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_PKA-mEGFP
Plasmid#233353PurposeCMV driven expression of the PKA activity recorder Kinprola_PKA in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_PKA-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_PKA_T/A-mEGFP
Plasmid#233354PurposeCMV driven expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA_T/A in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_PKA_T/A-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_JNK-mEGFP
Plasmid#233359PurposeCMV driven expression of the JNK activity recorder Kinprola_JNK in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_JNK-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_JNK_T/A-mEGFP
Plasmid#233360PurposeCMV driven expression of the inactive JNK activity recorder negative control with T/A mutation Kinprola_JNK_T/A in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_JNK_T/A-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_PKC_T/A-mEGFP
Plasmid#233358PurposeCMV driven expression of the inactive PKC activity recorder negative control with T/A mutation Kinprola_PKC_T/A in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_PKC_T/A-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_on-mEGFP
Plasmid#233355PurposeCMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_on-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_AMPK_T/A-mEGFP
Plasmid#233362PurposeCMV driven expression of the inactive AMPK activity recorder negative control with T/A mutation Kinprola_AMPK_T/A in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_AMPK_T/A-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_AMPK-mEGFP
Plasmid#233361PurposeCMV driven expression of the AMPK activity recorder Kinprola_AMPK in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_AMPK-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_Kinprola_PKA-mEGFP-MTBD
Plasmid#233363PurposeCMV driven expression of the PKA activity recorder Kinprola_PKA in mammalian cell lines fused to mEGFP, targeting to the microtubuleDepositorInsertKinprola_PKA-mEGFP-MTBD
UseTagsmEGFP-MTBDExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Kinprola_PKC-mEGFP
Plasmid#233357PurposeCMV driven expression of the PKC activity recorder Kinprola_PKC in mammalian cell lines fused to mEGFPDepositorInsertNES-Kinprola_PKC-mEGFP
UseTagsNES and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro_CMV_NES-Kinprola_PKA-mEGFP
Plasmid#233369PurposeCMV driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for neuronal expression through lentivirus transductionDepositorInsertNES-Kinprola_PKA-mEGFP
UseLentiviralTagsNES and mEGFPExpressionMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro_CMV_NES-Kinprola_on-mEGFP
Plasmid#233370PurposeCMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through lentivirus transductionDepositorInsertNES-Kinprola_on-mEGFP
UseLentiviralTagsNES and mEGFPExpressionMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-mEGFP-WIPI2
Plasmid#223757PurposeStable expression and inducible recruitment of protein in mammalian cell cultureDepositorInsertWIPI2d (WIPI2 Human)
UseLentiviralTagsFKBP-mEGFPExpressionMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-mEGFP-WIPI1
Plasmid#223767PurposeStable expression and inducible recruitment of protein in mammalian cell cultureDepositorInsertWIPI1 (WIPI1 Human)
UseLentiviralTagsFKBP-mEGFPExpressionMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-mEGFP-WIPI3
Plasmid#223768PurposeStable expression and inducible recruitment of protein in mammalian cell cultureDepositorInsertWIPI3 (WDR45B Human)
UseLentiviralTagsFKBP-mEGFPExpressionMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-mEGFP-WIPI4
Plasmid#223769PurposeStable expression and inducible recruitment of protein in mammalian cell cultureDepositorInsertWIPI4 (WDR45 Human)
UseLentiviralTagsFKBP-mEGFPExpressionMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIVEX2.3-CS1M2b-3c-ON8-EGFP
Plasmid#228452PurposeLS4-responsive riboswitchDepositorInsertCS1M2b-3c-ON8 riboswitch-regulated EGFP
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIVEX2.3-CS2-3c-ON8-EGFP
Plasmid#228453PurposeLS12-responsive riboswitchDepositorInsertCS2-3c-ON8 riboswitch-regulated EGFP
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG Retro EGFP Neo
Plasmid#234464PurposeControl retroviral vector expressing Green Fluorescent Protein. Neomycin selection.DepositorInsertGreen Fluorescent Protein
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMarch 21, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAG Retro EGFP Puro
Plasmid#234463PurposeControl retroviral vector expressing Green Fluorescent Protein. Puromycin selection.DepositorInsertGreen Fluorescent Protein
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMarch 21, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAG Retro EGFP Hygro
Plasmid#234462PurposeControl retroviral vector expressing Green Fluorescent Protein. Hygromycin selection.DepositorInsertGreen Fluorescent Protein
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMarch 21, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV.minBG-EGFP-W3SL_BbsI(GGA)
Plasmid#231362PurposeminBG-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.DepositorInsertEGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Ple155-CI-EGFP-W3SL_BbsI(GGA)
Plasmid#231363PurposePle155-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.DepositorInsertEGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.BC(p1-10)-Ple155-CI-EGFP-W3SL-BC(p1-10)
Plasmid#231351PurposeSingle stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from Ple155 element, active in Purkinje cells.DepositorInsertBC(p1-10)
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.BC(p1-10)-CAG-EGFP-W3SL-BC(p1-10)
Plasmid#231347PurposeSingle stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.DepositorInsertBC(p1-10)
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pscAAV.BC(p1-6)-CAG-EGFP-SV40pA-BC(p1-6)
Plasmid#231349PurposeSelf complementary AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.DepositorInsertBC(p1-10)
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
p49.unipolyA.Cdt1.mSca.mCMV.PGK.eGFP.Gem1.WPRE
Plasmid#226768Purposevector with mScarlet fluorescent protein and GFPDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only