We narrowed to 11,559 results for: NSI

1. pHR-mCherry-CRY2-NLS

   Plasmid

   #145259

   Purpose

   Mammalian Expression of Nuclear Control OptoConstruct with SV40 NLS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAB078d5

   Plasmid

   #79204

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) luxAB; Ppro1 434cI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacZ-opt (OR1); Ppro1

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPCK

   Plasmid

   #133128

   Purpose

   a recombinant plasmid, expressing pckA PEP-carboxykinase) gene

   Depositor

   Gregg Beckham

   Article

   Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   PEP-carboxykinase gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PEP-carboxykinase promoter

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. EK0285 SFFV-mCherry-SG(s70587)SG-EGFP (FLP-IN)

   Plasmid

   #191140

   Purpose

   Expression of Sensor 1 (for EK0208) in mammalian cells; mCherry marker, EGFP output.

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:s70587:EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-mito-Citroff1-S245

   Plasmid

   #134306

   Purpose

   mitochondria-targeted inverse-response citrate sensor variant with medium binding affinity

   Depositor

   Robert Campbell

   Article

   Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

   Insert

   Citroff1 S245A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Clomeleon

   Plasmid

   #203723

   Purpose

   A genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentration

   Depositor

   George Augustine

   Article

   Kuner et al Neuron. 2000 Sep;27(3):447-59. doi: 10.1

   Insert

   cyan fluorescent protein linked yellow florescent protein
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Cyan Fluorescent Protein, Topaz Fluorescent Prote…

   Promoter

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCRISPR V2 sgIREB2_2

   Plasmid

   #169886

   Purpose

   Disrupt IREB2

   Depositor

   Richard Possemato

   Article

   Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

   Insert

   sgRNA targeting IREB2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3-VEGFR2-780

   Plasmid

   #21307

   Depositor

   Donald Ingber

   Article

   Mammoto et al Nature. 2009 Feb 26. 457(7233):1103-8.

   Insert

   VEGFR2 promoter (KDR Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. EK0395 mCherry-SG(s70587)SG-EGFP (pEAQ)

   Plasmid

   #191170

   Purpose

   EK0285 in plant-expressable format: Sensor 1 (for EK0208); mCherry marker, EGFP output.

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:s70587:EGFP (plant)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   WT

   Promoter

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p12x601

    Plasmid

    #157785

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with central TetO following PEG removal of plasmid backbone

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    pMD2.G(spei/spei)&12x601_tetO
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSW454 - pUFO

    Plasmid

    #115978

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Jan Lohmann

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pUFO
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Flag Snail 6SA

    Plasmid

    #16221

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    Snail (SNAI1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S96A, S100A, S107A, S111A, S115A, S119A

    Promoter

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJV53-Cas12a

    Plasmid

    #158706

    Purpose

    CRISPR system used for genome editing in Mycobacterium smegmatis, expresses codon-optimized FnCpf1 and the recombination proteins gp60 and gp61

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRK5-mFzd3

    Plasmid

    #42255

    Depositor

    Chris Garcia, Jeremy Nathans

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 3 (Fzd3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pAB094a

    Plasmid

    #79241

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PBAD rpoZ-HA4; PC araC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD; PC

    Available Since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAROTENE5X

    Plasmid

    #208816

    Purpose

    Encodes bacterial crtEBIY operon under the control of synthetic promoter responsive to JUB1-derived artificial transcription factor in bacterial cell

    Depositor

    Marc Erhardt

    Article

    Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:

    Insert

    Five copies of JUB1 binding site within the synthetic promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    JUB1 TF-responsive promoter

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMS178

    Plasmid

    #215678

    Purpose

    Empty repair plasmid for ChrII split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    5'HA + MCS + loxP + rps-0p::5'ΔHygR
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    5' ∆HYGR encodes aa1-226

    Promoter

    Available Since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMS114

    Plasmid

    #215677

    Purpose

    Empty repair plasmid for ChrI split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    5'HA + MCS + loxN + rps-0p::5'ΔHygR
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    5' ∆HYGR encodes aa1-226

    Promoter

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAID1.2-EF1a-NmScarlet-mAID

    Plasmid

    #140617

    Purpose

    Expression of OsTIR1 and mScarlet-mAID protein in mammalian cells under the control of EF1a promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Shp2 biosensor

    Plasmid

    #134346

    Purpose

    FRET biosensor. To visualize Shp2 activity by FRET

    Depositor

    Yingxiao Wang

    Article

    Sun et al Nat Commun. 2017 Sep 7;8(1):477. doi: 10

    Insert

    Shp2 Biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLentiGuide_mCherry_NT135

    Plasmid

    #183121

    Purpose

    pLentiGuide modified to express mCherry and a non-targeting sgRNA

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    NT135
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAV-U6+27-Tornado-Squash-SAM-sensor 5-1

    Plasmid

    #177917

    Purpose

    Expresses Squash-SAM sensor 5-1 as circular RNA in mammalian cells using the Tornado expression system.

    Depositor

    Samie Jaffrey

    Article

    Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

    Insert

    Tornado-Squash-SAM-sensor 5-1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28sumo-hRig-I DCARDS

    Plasmid

    #210828

    Purpose

    protein expression in ecoli

    Depositor

    Anna Pyle

    Article

    Luo et al Cell. 2011 Oct 14;147(2):409-22. doi: 10

    Insert

    hRIG-I DCARDS (DDX58) with Caspase Recruitment domains deleted (RIGI Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGWB508-cTP-sfGFP

    Plasmid

    #213863

    Purpose

    Binary vector for the expression of sfGFP flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

    Insert

    Plastid transit peptide fused sfGFP
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFastBac_human-IL-18

    Plasmid

    #214338

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pWM_12x601_193 NRL + TetO

    Plasmid

    #157812

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_tetO
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-EFS-DIO-GRAB_ATP1.0

    Plasmid

    #167581

    Purpose

    Express the ATP sensor GRAB_ATP1.0 in Cre positive cells

    Depositor

    Yulong Li

    Article

    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

    Insert

    ATP sensor GRAB_ATP1.0 (P2RY1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p416 25Q GPD

    Plasmid

    #1177

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 25Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MsACR1-mCerulean3-pAAV2-hSyn

    Plasmid

    #204965

    Purpose

    Panneuronal expression of MsACR1 fused to mCerulean3

    Depositor

    Peter Hegemann

    Article

    Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

    Insert

    MsACR1
    

    Use

    AAV

    Tags

    mCerulean3

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-anti-GFP(LaG16) PAGER(Gi)

    Plasmid

    #230008

    Purpose

    Expresses anti-GFP PAGER(Gi) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    MT1-LaG16-TEVcs-ALFA-hM4D
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAID1.2-EF1a-CmCherry-mAID

    Plasmid

    #140604

    Purpose

    Expression of OsTIR1 and mAID-mCherry protein in mammalian cells under the control of EF1a promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLV-TetOn-NLS-mCherry

    Plasmid

    #193762

    Purpose

    Doxycycline-inducible (TetOn) mCherry with SV40 NLS, control construct

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    NLS-mCherry
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    SV40 NLS fused to mCherry

    Promoter

    TRE

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pARF6-YFP

    Plasmid

    #11389

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    ADP Ribosylation Factor 6 (ARF6 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p416 103Q GPD

    Plasmid

    #1180

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 103Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

35. pLentiCRISPR_Puro_sgAAVS1

    Plasmid

    #187818

    Purpose

    pLentiCRISPR that is selectable with puromycin with an sgRNA targeting AAVS1

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    sgAAVS1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mNeonGreen-Smad3

    Plasmid

    #172098

    Purpose

    Expresses fluorescently tagged human Smad3 in mammalian cells.

    Depositor

    Lea Goentoro

    Article

    Frick et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Insert

    Smad 3 (Smad3 Mouse)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    C49S mutation in mCerulean

    Promoter

    CMV

    Available Since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAUC40

    Plasmid

    #71298

    Purpose

    Suicide Vector, Gateway attR-Chloramphenicol resistance cassette cloned in pKNG101

    Depositor

    Susanne von Bodman

    Article

    Carlier et al Mol Microbiol. 2009 Nov;74(4):903-13. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCS2 stabilized mutant Beta Catenin-GFP

    Plasmid

    #29684

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    beta catenin (ctnnb1.L Frog)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S33A, S37A, T41A

    Promoter

    Available Since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

39. pGWB508-cTP-mCherry

    Plasmid

    #213862

    Purpose

    Binary vector for the expression of mCherry flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

    Insert

    Plastid transit peptide fused mCherry-UnaG
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. GFP Snail 6SA

    Plasmid

    #16228

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    Snail (SNAI1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    6SA: S97A, S101A, S108A, S112A, S116A, S120A

    Promoter

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge