We narrowed to 7,223 results for: ALP
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Plasmid#56349PurposeLocalization: Mitochondria, Excitation: 530, Emission: 541DepositorInsertPDHA1 (PDHA1 Human)
UseTagsmPapayaExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNLRP11minor.MYC
Plasmid#131195PurposeMammalian expression of human NLRP11 188ADepositorInsertNLRP11 188A (NLRP11 Human)
UseTagsmycExpressionMammalianMutation188APromoterpCMVAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TCF3
Plasmid#67110PurposeStandard for cell-free expression benchmarking.DepositorInsertTCF3 (TCF3 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationTruncated isoformPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAB043c
Plasmid#79223PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ lambda cI-p38-alpha; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPlacZ; PlacIqAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
Col7A1-CRISPR-gRNA#exon2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)
Plasmid#124286PurposesgRNA against human Col7A1DepositorInsertCollagen type VII alpha 1 chain (COL7A1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
mPapaya1-Cx43-7
Plasmid#56649PurposeLocalization: Gap Junctions, Excitation: 530, Emission: 541DepositorInsertCx43
UseTagsmPapaya1ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBOB-HA-Gnaq
Plasmid#236750PurposeExpress HA N-terminal tagged mouse Gq transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.DepositorInsertGnaq (Gnaq Mouse)
UseLentiviralTagsHAExpressionMammalianMutationPromoterAvailable sinceMay 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTripZ-Tet-ON mCherry
Plasmid#187262PurposeDoxycyclin inducible expression of mCherryDepositorInsertmCherry-NLS
UseLentiviralTagsmCherryExpressionMutationPromoterCMVAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
EKAREN5-gl
Plasmid#225958PurposeThe YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.DepositorInsertEKAREN5-gl
UseLentiviralTagsnls localization motifExpressionMammalianMutationPromoterEF-1-alpha promoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
GB4653
Plasmid#215267PurposeTU for the constitutive expression of the flippase (FLP) recombinase with a nopaline synthase (Nos) promoterDepositorInsertpNos:FLP:tNos
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
GB4829
Plasmid#215275PurposeTU for the constitutive expression of the N. nambi NPGA gene under the P35S promoter and the TNOS terminatorDepositorInsertP35S:NPGA:TNOS
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMET7-GAG-FRB
Plasmid#194455PurposeMammalian expression vector that expresses the HIV-1 GAG protein with FRB fused to itDepositorInsertFRB (MTOR Human)
UseTagsGAGExpressionMammalianMutationPromoterSR alphaAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAS_pb_Ef1a-TIR1_Ubc-mCherry-Puro
Plasmid#195567PurposePiggyBac plasmid for stable expression of TIR1, mCherry, and Puromycin resistance in mammalian cellsDepositorInsertTIR1
UseTagsExpressionMammalianMutationPromoterEf1-alphaAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4832
Plasmid#215276PurposeModule for the constitutive expression of HISPS, H3H, EGFP and p23.DepositorInsertHISPS-H3H-EGFP-p23
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-APP-CTF
Plasmid#181732PurposeGalactose inducible expression of APP-CTFDepositorInsertAPP-CTF (APP Human)
UseTagsExpressionYeastMutationC-terminal fragment (aa 671-770)PromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV UBC msNLRP3(LRRm2)-APEX2-3xFLAG-IRES-eGFP (N1008R_R1009E_E1010R_R1013E)
Plasmid#218635PurposeProximity biotinylation of msNLRP3(LRRm2, N1008R_R1009E_E1010R_R1013E)DepositorInsertNLRP3 (Nlrp3 Mouse)
UseLentiviralTagsAPEX2-3xFLAGExpressionMammalianMutationN1008R, R1009E, E1010R, R1013EPromoterUBCAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-TurboID-GFP
Plasmid#209399PurposeTransiently expressing and visualizing the subcellular localization of TurboID in plantaDepositorInsert3XHA-TurboID
UseTagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRAPa(R150A)-3xFLAG-pCDNA
Plasmid#201966PurposeMammalian expression of mutated Translocon-associated protein subunit alphaDepositorInsertSSR1 (SSR1 Human)
UseTags3xFLAG-tagExpressionMammalianMutationR150APromoterAvailable sinceJan. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
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SGTA-2Strep
Plasmid#210353PurposeExpresses SGTA fused with 2Strep in mammalian cellsDepositorInsertSGTA (SGTA Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB395
Plasmid#203631PurposeTU for the expression of luciferase under a synthetic promoter containing the target sequence for gRNA1 (1xLuc).DepositorInsertR1:G1aG2b.1:mPAF:Luc:TtrpC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB396
Plasmid#203632PurposeTU for the expression of luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc).DepositorInsertR1:G1ab.1:mPAF:Luc:TtrpC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB397
Plasmid#203633PurposeTU for the expression of luciferase under a synthetic promoter containing three copies of the target sequence for gRNA1 (3xLuc).DepositorInsertR1:G1abc.3:mPAF:Luc:TtrpC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmEGFP-tHe-Tub1C1
Plasmid#201730PurposemEGFP-tagged tardigrade (Hypsibius exemplaris) alpha-tubulin Tub1C1 for expression in mammalian cellsDepositorInserttHe-Tub1C1
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmEGFP-tHe-Tub1A2
Plasmid#201729PurposemEGFP-tagged tardigrade (Hypsibius exemplaris) alpha-tubulin Tub1A2 for expression in mammalian cellsDepositorInserttHe-Tub1A2
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmEGFP-tHe-Tub1A1
Plasmid#201728PurposemEGFP-tagged tardigrade (Hypsibius exemplaris) alpha-tubulin Tub1A1 for expression in mammalian cellsDepositorInserttHe-Tub1A1
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSin_EF1a_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR
Plasmid#176310PurposeExpresses CreN59-nMag-NLS with mCherry marker.DepositorInsertCreN59_nMag_NLS_P2A_mCherry_IRES_puroR
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman EF1alphaAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMET7-GAG-eDHFR
Plasmid#194635PurposeMammalian expression of the eDHFR control bait in VirotrapDepositorInsertp55 GAG
UseTagsecDHFRExpressionMammalianMutationPromoterSRalphaAvailable sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF1α -NWM-Node 1
Plasmid#154253Purposeexpresses reconstructed New World monkey retroCHMP3 Node 1 in mammalian cellsDepositorInsertreconstructed Node 1 of New World monkey retroCHMP3
UseTagsHAExpressionMammalianMutationPromoterEF1-alphaAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1α -NWM-Node 2
Plasmid#154254Purposeexpresses reconstructed New World monkey retroCHMP3 Node 2 in mammalian cellsDepositorInsertreconstructed Node 2 of New World monkey retroCHMP3
UseTagsHAExpressionMammalianMutationPromoterEF1-alphaAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1α -NWM-Node 3
Plasmid#154255Purposeexpresses reconstructed New World monkey retroCHMP3 Node 3 in mammalian cellsDepositorInsertreconstructed Node 3 of New World monkey retroCHMP3
UseTagsHAExpressionMammalianMutationPromoterEF1-alphaAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-wbp2nlF127P
Plasmid#174507Purposeexpresses protein with one aa substitution to disrupt the alpha-helix near the PH-G domainDepositorInsertwbp2nlF127P
UseXenopus expressionTagsnoneExpressionMutationchanged Phenylalanine at aa127 to ProlinePromoterSP6Available sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C453-E21: attB4-FerHp>-attB5
Plasmid#162978PurposeGateway attB4/attB5 entry clone for mammalian expression using the human EF1alpha promoterDepositorInserthuman Ferritin heavy chain promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E11: attB4-Grp78p>-attB1r
Plasmid#162970PurposeGateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoterDepositorInserthuman Glucose response protein 78 promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E10: attB4-FerHp>-attB1r
Plasmid#162969PurposeGateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoterDepositorInserthuman Ferritin heavy chain promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54 no FG-mEGFP494(2)
Plasmid#164271PurposeMammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationdeleted the FG region of Nup54PromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54 no FG-mEGFP494(1)
Plasmid#164270PurposeMammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationdeleted the FG region of Nup54PromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFlox_N-BRM_BSD_FKBPF36V_EYFPF36V
Plasmid#163876PurposeAcute Depletion of BRM-EYFPDepositorInsertBrm (Smarca2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4xh7SKPylT_EF1_BsmBI_(C1A)-Intein_DD
Plasmid#162811PurposeControl STELLA destination plasmid for C-terminal labelled proteins. Contains restriction sites for golden gate cloning.DepositorInsertBsmBI_(C1A)-Intein_DD
UsePiggybacTagsExpressionMammalianMutationIntein(Cys1,Ala)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_M
Plasmid#162815PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_MDepositorInsertUb-(N-TAG)CoV2_M
UsePiggybacTagsExpressionMammalianMutationCoV2_M(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_9b
Plasmid#162816PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_9bDepositorInsertUb-(N-TAG)CoV2_9b
UsePiggybacTagsExpressionMammalianMutationCoV2_9b(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF6
Plasmid#162817PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF6DepositorInsertUb-(N-TAG)CoV2_ORF6
UsePiggybacTagsExpressionMammalianMutationCoV2_ORF6(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF7b
Plasmid#162818PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF7bDepositorInsertUb-(N-TAG)CoV2_ORF7b
UsePiggybacTagsExpressionMammalianMutationCoV2_ORF7b(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF10
Plasmid#162819PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF10DepositorInsertUb-(N-TAG)CoV2_ORF10
UsePiggybacTagsExpressionMammalianMutationCoV2_ORF10(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF9c/14
Plasmid#162820PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF9c/14DepositorInsertUb-(N-TAG)CoV2_ORF9c/14
UsePiggybacTagsExpressionMammalianMutationCoV2_ORF9c/14(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b_alt
Plasmid#162821PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b_altDepositorInsertUb-(N-TAG)CoV2_ORF3b_alt
UsePiggybacTagsExpressionMammalianMutationCoV2_ORF3b_alt(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b(CoV1h)
Plasmid#162822PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b(CoV1h)DepositorInsertUb-(N-TAG)CoV2_ORF3b(CoV1h)
UsePiggybacTagsExpressionMammalianMutationCoV2_ORF3b(CoV1h)(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD
Plasmid#162805PurposeSTELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integrationDepositorInsertUb-BsmBI-HA_IRES_Blasticidin
UsePiggybacTagsHAExpressionMammalianMutationPromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BufAI-2
Plasmid#128651PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BufAI-1
Plasmid#128650PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only