We narrowed to 949 results for: Streptococcus

1. pDAC627

   Plasmid

   #195240

   Purpose

   Mammalian expression of Csm complex from separate promoters; Puro backbone (Used for RNA knockdown)

   Depositor

   Jennifer Doudna

   Article

   Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

   Insert

   FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; Puro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKIR1.0

   Plasmid

   #86409

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Expression

   Mutation

   Promoter

   AtRPS5A

   Available since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDAC446

   Plasmid

   #195239

   Purpose

   Mammalian expression of Csm complex from separate promoters; GFP backbone (Used for RNA knockdown)

   Depositor

   Jennifer Doudna

   Article

   Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

   Insert

   FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(-)-HA-SrtAstrep (pKS77)

   Plasmid

   #50030

   Purpose

   Expression of cytosolic S. pyogenes SrtA in mammalian cells

   Depositor

   Hidde Ploegh

   Article

   Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

   Insert

   SrtA(strep)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7sgRNA

   Plasmid

   #111820

   Purpose

   Expresses sgRNA in T. brucei cells

   Depositor

   David Horn

   Article

   Rico et al Sci Rep. 2018 May 21;8(1):7960. doi: 10.

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKI1.1

   Plasmid

   #85807

   Purpose

   CRISPR/Cas9 for Arabidopsis in pENTR/D-TOPO

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Expression

   Mutation

   Promoter

   AtRPS5A

   Available since

   May 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJWV102-wtcas9sp

   Plasmid

   #99612

   Purpose

   Plasmid containing wild-type cas9sp

   Depositor

   Jan-Willem Veening, Morten Kjos

   Article

   van Raaphorst et al Proc Natl Acad Sci U S A. 2017 Jul 3. pi

   Insert

   cas9sp
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PczcD

   Available since

   Sept. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-SacB-Cas9-T2A-mCherry_(BbsI)

   Plasmid

   #117070

   Purpose

   Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection

   Depositor

   Kim Failor

   Inserts

   Cas9
   SacB
   

   Use

   CRISPR

   Tags

   3x FLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMK17-01

   Plasmid

   #99603

   Purpose

   Plasmid containing the parB-m(sf)gfp fusion

   Depositor

   Jan-Willem Veening, Morten Kjos

   Article

   van Raaphorst et al Proc Natl Acad Sci U S A. 2017 Jul 3. pi

   Insert

   parBp
   

   Use

   Synthetic Biology

   Tags

   m(sf)gfp

   Expression

   Mutation

   Promoter

   PczcD

   Available since

   Sept. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMK11-02

    Plasmid

    #99606

    Purpose

    Plasmid containing parBp(mut)-mKate2 fusion

    Depositor

    Jan-Willem Veening, Morten Kjos

    Article

    van Raaphorst et al Proc Natl Acad Sci U S A. 2017 Jul 3. pi

    Insert

    parBp(mut)-mKate2
    

    Use

    Synthetic Biology

    Tags

    mKate2

    Expression

    Mutation

    internal parS-site mutated

    Promoter

    PczcD

    Available since

    Sept. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCSW24

    Plasmid

    #86837

    Purpose

    bacterial expression of anti-CRISPR protein AcrIIA3

    Depositor

    Joseph Bondy-Denomy

    Article

    Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

    Insert

    acrIIA3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Oct. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS303-pGAL-HA-SrtAstrep (pKS78)

    Plasmid

    #50029

    Purpose

    Galactose-inducible expression of cytosolic S. pyogenes SrtA in yeast

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

    Insert

    HA-SrtA(strep)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    pGAL

    Available since

    Jan. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRS303-pGAL-HA-TMSec66-SrtA (pKS105)

    Plasmid

    #50033

    Purpose

    Galactose-inducible expression of ER-membrane S. pyogenes SrtA in yeast

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

    Insert

    SrtA(strep)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    pGAL

    Available since

    Jan. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. A9QH69

    Plasmid

    #163216

    Purpose

    Inducible expression of UniProt identifier A9QH69

    Depositor

    Martin Engqvist

    Article

    Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

    Insert

    A9QH69
    

    Use

    Tags

    10xHis and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDAC565

    Plasmid

    #195242

    Purpose

    Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCas9-polyA

    Plasmid

    #72602

    Purpose

    express hCas9 byT7 promoter with polyadenine tails

    Depositor

    Tomoji Mashimo

    Article

    Yoshimi et al Nat Commun. 2016 Jan 20;7:10431. doi: 10

    Insert

    Cas9-polyadenine
    

    Use

    Tags

    81-bp polyadenylation

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX165-HiFi Cas9

    Plasmid

    #140563

    Purpose

    Expresses HiFi Cas9 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mutation

    R691A

    Promoter

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSECRETS-A

    Plasmid

    #196986

    Purpose

    For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).

    Depositor

    Eric Josephs

    Article

    Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pltetO1

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDAC439

    Plasmid

    #195237

    Purpose

    Mammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHO4d-Cas9

    Plasmid

    #67881

    Purpose

    Cas9nlsHis6 Bacterial Expression construct

    Depositor

    Michael Nonet

    Article

    Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and T7 epitope

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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