We narrowed to 7,248 results for: Cit

1. pl-Synapsin-YFP-NaVII-III

   Plasmid

   #91246

   Purpose

   Expresses YFP-NaVII-III from the Synapsin promoter, for neuron-specific and activity-independent live label of the Axon Initial Segment (AIS)

   Depositor

   Matthew Grubb

   Article

   Dumitrescu et al Front Cell Neurosci. 2016 Nov 23;10:268.

   Insert

   Nav1.2 (Scn2a Rat)
   

   Use

   Lentiviral

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   The insert codes only for the intracellular loop …

   Promoter

   Available since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. MBP-FUS_LC_WT

   Plasmid

   #98653

   Purpose

   Expresses MBP-tagged FUS AA 1-163

   Depositor

   Nicolas Fawzi

   Article

   Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. MBP-FUS_FL_12E

   Plasmid

   #98652

   Purpose

   Expresses MBP-tagged full length phosphomimetic FUS

   Depositor

   Nicolas Fawzi

   Article

   Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   12 S/T to E

   Promoter

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLEX307-SARS-CoV-2-3CL

   Plasmid

   #160278

   Purpose

   Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pES001 MBP-GSK3β-HA-His

   Plasmid

   #154010

   Purpose

   Expresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   GSK3B (GSK3B Human)
   

   Use

   Tags

   HA tag, His6, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmCherryC2-Gephyrin P1

   Plasmid

   #68820

   Purpose

   mCherry tagged Geph for mammalian expression

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al Proc Natl Acad Sci U S A. 2011 Jan 4;108

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   silent mutations on internal EcoRI site. Gephyrin…

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. xCas12i

   Plasmid

   #195333

   Purpose

   vector for encoding a human codon-optimized xCas12i driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

   Insert

   human codon-optimized xCas12i
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CBh, CMV, hU6

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Syn-ChrimsonR-GFP

   Plasmid

   #58806

   Purpose

   AAV expression of ChrimsonR-GFP under the Syn promoter

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   K176R

   Promoter

   Syn

   Available since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Syn-CoChR-GFP

   Plasmid

   #59070

   Purpose

   AAV-mediated expression of CoChR-GFP under the Syn promoter

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   CoChR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Syn

   Available since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL3-TK-5UTR-BsmBI-Luciferase

    Plasmid

    #114670

    Purpose

    For one-step cloning (with blue/ white screening) of a desired 5' UTR to test its impact on translation of a firefly luciferase reporter

    Depositor

    Lynne Postovit

    Article

    Jewer et al Nat Commun. 2020 May 19;11(1):2498. doi:

    Insert

    LacZ
    

    Use

    Luciferase

    Tags

    for cloning 5' UTR-Luciferase fusions

    Expression

    Mammalian

    Mutation

    Promoter

    lac promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103_HCK-1/1

    Plasmid

    #91273

    Purpose

    Protein expression and purification of human SH3 domain construct HCK-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    HCK-1/1 (HCK Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5FRT/Flag-FLJ10081

    Plasmid

    #64775

    Purpose

    Mammalian expression of Flag-tagged FLJ10081 (NSL3)

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

    Insert

    FLJ10081 (KANSL3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDYT001

    Plasmid

    #186549

    Purpose

    Lineage tracing vector (with barcoded Target Sites and triple sgRNAs)

    Depositor

    Jonathan Weissman

    Article

    Yang et al Cell. 2022 Apr 28. pii: S0092-8674(22)00

    Insert

    14-bp random integration barcode and three target sites and 3x sgRNAs
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-syn-synaptoPAC-minWPRE

    Plasmid

    #153085

    Purpose

    Expresses synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neurons

    Depositor

    Dietmar Schmitz

    Article

    Oldani et al J Neurochem. 2021 Feb;156(3):324-336. do

    Inserts

    bPAC(WT)
    Synaptophysin (Syp Rat)
    

    Use

    AAV

    Tags

    His tag, mScarlet, and myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. p3HAT1Luc1

    Plasmid

    #236275

    Purpose

    Mammalian expression of human angiotensin II type 1 receptor; N-terminal HA tag, C-terminal Rluc8

    Depositor

    Vsevolod Gurevich

    Article

    Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

    Insert

    angiotensin II type 1 receptor (AGTR1 Human)
    

    Use

    Tags

    3xHA and Rluc8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX NPC1-FLAG-TiD

    Plasmid

    #199748

    Purpose

    Lentiviral construct to stably express NPC1 TurboID

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Tags

    Flag and TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3 mHIF-2α MYC (P405A/P530V/N851A)

    Plasmid

    #44027

    Depositor

    Celeste Simon

    Article

    Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

    Insert

    mHIF-2α (P405A/P530V/N851A) (Epas1 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    P405A, P530V, N851A

    Promoter

    CMV

    Available since

    March 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. 426Gal-FUS-YFP

    Plasmid

    #29592

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. Linked Free ClLIS1 (M)

    Plasmid

    #182487

    Purpose

    Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-ClLIS1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Insert

    FPPS(M)-LS
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenSyn2-EGFP-­OMP25C

    Plasmid

    #71427

    Purpose

    Lentiviral expression of EGFP-OMP25C. For visualization of neuronal mitochondria.

    Depositor

    Henri Huttunen

    Article

    Kysenius et al PLoS One. 2014 Sep 5;9(9):e107129. doi:

    Insert

    OMP25 (172-­‐206) (Synj2bp Rat)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 172-206 only

    Promoter

    synapsin

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

21. MBP-FUS_FL_6E

    Plasmid

    #98655

    Purpose

    Expresses MBP-tagged full length phosphomimetic FUS

    Depositor

    Nicolas Fawzi

    Article

    Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    6 S/T to E

    Promoter

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHH0103_BTK-1/1

    Plasmid

    #91350

    Purpose

    Protein expression and purification of human SH3 domain construct BTK-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    BTK-1/1 (BTK Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CMV-VPR-S-L1-dCjCas9 MiniCAFE

    Plasmid

    #169910

    Purpose

    Expression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Insert

    MiniCAFE
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D8A, HNH-truncation (Δ495–609 aa) in CjCas9

    Promoter

    CMV

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pKH3-TRIM28-C65A/C68A

    Plasmid

    #92199

    Purpose

    Mammalian overexpression vector for TRIM28; catalytically inactive (mutated on C65A and C68A)

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    Tif1b (TRIM28 Human, Synthetic)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Changed Cysteines 65 and 68 to Alanines.

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHH0103_GRB2-1/2

    Plasmid

    #91480

    Purpose

    Protein expression and purification of human SH3 domain construct GRB2-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    GRB2-1/2 (GRB2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-linker-TlcnC

    Plasmid

    #114377

    Purpose

    expresses Flpo recombinase-dependent GtACR2-Kv2.1C-linker-TlcnC, which targets GtACR2 to the somatodendritic compartment. Messier et al found this hybrid construct was the most effective at targeting

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP-Kv2.1C-linker-TlcnC (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP and Kv2.1C-linker-TlcnC

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHH0103_ITK-1/1

    Plasmid

    #91253

    Purpose

    Protein expression and purification of human SH3 domain construct ITK-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ITK-1/1 (ITK Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LT3GEPIR-ARshRNA2

    Plasmid

    #220562

    Purpose

    To inducibly knockdown AR expression

    Depositor

    Sean Lee

    Article

    Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

    Insert

    AR shRNA2 (AR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGAL4-DBD-FOXA3_AroLITE

    Plasmid

    #215640

    Purpose

    For overexpression of Gal4-DBD FOXA3 AroLITE

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    FOXA3 AroLITE (FOXA3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGAL4-DBD-FOXA3_AroPERFECT

    Plasmid

    #215641

    Purpose

    For overexpression of Gal4-DBD FOXA3 AroPERFECT

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    FOXA3 AroPERFECT (FOXA3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge