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Plasmid#227902PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertPEX3 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML318_SEC23A-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227903PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertSEC23A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML319_SEC31A-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227904PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertSEC31A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML317_COPE-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227905PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertCOPE homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_eGFP-pScaE
Plasmid#228826PurposeFor the expression of the passenger domain of the Orientia tsutsugamushi protein ScaE in HeLa Flp-In cellsDepositorInsertScaE
UseTagseGFPExpressionMammalianMutationaa 49-494PromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_eGFP-pScaD
Plasmid#228825PurposeFor the expression of the passenger domain of the Orientia tsutsugamushi protein ScaD in HeLa Flp-In cellsDepositorInsertScaD
UseTagseGFPExpressionMammalianMutationaa 30-700PromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_eGFP-pScaC
Plasmid#228824PurposeFor the expression of the passenger domain of the Orientia tsutsugamushi protein ScaC in HeLa Flp-In cellsDepositorInsertScaC
UseTagseGFPExpressionMammalianMutationaa 33-223PromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_eGFP-pScaA
Plasmid#228823PurposeFor the expression of the passenger domain of the Orientia tsutsugamushi protein ScaA in HeLa Flp-In cellsDepositorInsertScaA
UseTagseGFPExpressionMammalianMutationaa 25-1216PromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GfaABC1D-eGFP-2A-mCherry_D4H
Plasmid#194878PurposeRatiometric D4H sensor for in vivo astrocyte-specific cholesterol visualizationDepositorInsertGFP-T2A-mCherry-D4H
UseAAVTagsExpressionMammalianMutationPromoterGfaABC1DAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GfaABC1D-eGFP-2A-mCherry_YDA
Plasmid#194879PurposeRatiometric YDA sensor for in vivo astrocyte-specific cholesterol visualizationDepositorInsertGFP-T2A-mCherry-YDA
UseAAVTagsExpressionMammalianMutationPromoterGfaABC1DAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAcBac3-EcTrp-TGA-EGFP**
Plasmid#228786PurposeEcTrp aaRS/tRNA system for TGA suppression with EGFP containing two stop codons for dual suppressionDepositorInsertsEcTrpRS
EcWtR
EGFP**
UseBaculoviralTagsExpressionMammalianMutationTGA suppressorPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
OSCA1.2-K435L-L438K-peGFP-N1
Plasmid#228587PurposeExpresses OSCA1.2-K435L-L438K-eGFP in mammalian cellsDepositorInserthyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)
UseTagsExpressionMammalianMutationK435L, L438KPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-167:CDH5-mEGFP
Plasmid#217425PurposeHomology arms and mEGFP sequence for C-terminus tagging of human cadherin 5DepositorInsertCDH5 Homology Arms with mEGFP (CDH5 Human)
UseCRISPR; Donor templateTagsmEGFPExpressionMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceNov. 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCS2-MfnG-EcYRS-EGFP*
Plasmid#191119PurposeA plasmid for incorporation of O-Me-Tyr into an EGFP reporter without O-Me-Tyr feeding for zebrafishDepositorInsertMfnG - P2A - Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein
UseTagsHis tagExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-RELA-PGK-EGFP
Plasmid#228051PurposeHomologous donor template for RELA knockout expressing EGFPDepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterPGKAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-RELB-PGK-EGFP
Plasmid#228052PurposeHomologous donor template for RELB knockout expressing EGFPDepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterPGKAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-RELC-PGK-EGFP
Plasmid#228053PurposeHomologous donor template for RELC knockout expressing EGFPDepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterPGKAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-2O-crEGFP_EF1a-BFP
Plasmid#224784PurposeEGFP targeting crRNA for RfxCas13d expressed from hU6-2xTetO promoter and non-target BFP protein expressed from EF1a promoterDepositorInsertcrEGFP
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhU6-2xTetOAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-CrEGFP_EF1a-BFP
Plasmid#224786PurposeEGFP targeting crRNA for RfxCas13d expressed from hU6 promoter and non-target BFP protein expressed from EF1a promoterDepositorInsertcrBFP
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhU6 and hU6-2xTetOAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TOgw-C-mEGFP
Plasmid#223953PurposeGateway cloning of genes of interest with a C-terminal mEGFP fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TOgw-N-mEGFP
Plasmid#223949PurposeGateway cloning of genes of interest with an N-terminal mEGFP fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-AGG-NLS-EGFP
Plasmid#214602Purposeexpress EGFP ; in vitro transcribed modified RNA vectorDepositorInsertEGFP
UseUnspecifiedTagsExpressionMutationPromoterT7Available sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-H1-shSRGAP2B/C#1-hsynapsin-EGFP
Plasmid#220796PurposeshRNA plasmid against human SRGAP2B/C genes (#1) with EGFP expression in neuronsDepositorInsertEGFP
UseTagsExpressionMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Ctr1-gRNA-EGFP
Plasmid#226000PurposeNegative control for CRISPRi-KDDepositorInsertnegtive control sgRNA of no specific targets in human genome
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Ctr2-gRNA-EGFP
Plasmid#226001PurposeNegative control for CRISPRi-KDDepositorInsertnegtive control sgRNA of no specific targets in human genome
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-hL1-gRNA1-EGFP
Plasmid#226002PurposeCRISPRi-KD of human LINE1DepositorInsertsgRNA targeting human L1HS
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-hL1-gRNA2-EGFP
Plasmid#226003PurposeCRISPRi-KD of human LINE1DepositorInsertsgRNA targeting human L1HS
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NCL-gRNA1-EGFP
Plasmid#226004PurposeCRISPRi-KD of human NucleolinDepositorInsertsgRNA targeting human Nucleolin (NCL Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NCL-gRNA2-EGFP
Plasmid#226005PurposeCRISPRi-KD of human NucleolinDepositorInsertsgRNA targeting human Nucleolin (NCL Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-EGFP-GAPDH-R rich
Plasmid#225480PurposeExpress mEGFP-GAPDH-Rrich fusionDepositorInsertGAPDH fused with R-rich region of TDRD3 (GAPDH Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-EGFP-GAPDH-Tudor
Plasmid#225479PurposeExpress mEGFP-GAPDH-Tudor fusionDepositorInsertGAPDH fused with Tudor domain of TDRD3 (GAPDH Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-EGFP-GAPDH-RGG
Plasmid#225477PurposeExpress mEGFP-GAPDH-RGG fusionDepositorInsertGAPDH fused with RGG domain of TOP3B (GAPDH Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEZYeGFP-SARS-CoV-2E stop
Plasmid#224581PurposeTo express a GFP tagged version of the Envelope protein of SARS-CoV-2 lacking its PBM in mammalian cellsDepositorInsertSARS-CoV-2E stop (E SARS-CoV-2)
UseTagsGFPExpressionMammalianMutationstop codon before PBMPromoterCMVAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) EGFP-RalA(G23C)
Plasmid#224275Purposecell transfection and expreswsion of RalA (G23C)DepositorInsertRalA (RALA Human)
UseTagsExpressionMammalianMutationG23CPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP:HA-mTREK-1(tandem wt-wt)
Plasmid#224776PurposeMammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linkerDepositorInsertTandem TREK-1 (wt-wt) (Kcnk2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP:HA-mTREK-1(S131C)
Plasmid#224774PurposeMammalian expression vector for generating mouse TREK-1DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseTagsExpressionMammalianMutationS131CPromoterCMVAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-eGFP-msMMS22L
Plasmid#221014Purposemammalian expression vector of eGFP tagged mouse MMS22LDepositorInsertMMS22L (Mms22l Mouse)
UseTagseGFPExpressionMammalianMutationnaturally siResistant to siMMS22L (human) aagacuu…PromoterCMVAvailable sinceSept. 5, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pT7-NSP3SA11-F2A-EGFP
Plasmid#220808PurposeExpress separately the Rotavirus SA11 NSP3 protein and the original enhanced GFP (Yang et al., 1996)DepositorInsertRotavirus SA11 NSP3-F2A-EGFP
UseOtherTagsExpressionMutationPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-EGFP-IRES(Neo)R
Plasmid#158068PurposeeGFP under the TRE3G promoter, Neo resistance. For cloning GOIDepositorInserteGFP
UseTagseGFPExpressionMammalianMutationPromoterTRE3GAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/T0-EGFP-GNB1L
Plasmid#222621PurposeMammalian expression vector of flag-tagged GNB1LDepositorInsertGNB1L (GNB1L Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits