We narrowed to 59,329 results for: cloning

1. pShuttle-CMV-GFP U6promoter

   Plasmid

   #128049

   Purpose

   Adenoviral shuttle vector for shRNA expression from U6 promoter with GFP marker

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Adenoviral

   Expression

   Mammalian

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHyVec7

   Plasmid

   #34793

   Depositor

   Rob Steele

   Type

   Empty backbone

   Use

   Hydra expression

   Tags

   GFP, RFP, and intergenic region between Hydra rep…

   Promoter

   Hydra vulgaris actin promoter

   Available Since

   Jan. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTALEN(Nd154C63) SFFV

   Plasmid

   #50431

   Purpose

   pTAL plasmid compatible with the Golden Gate TALEN cloning system, for cloning of TALENs and expression in mammalian cells.

   Depositor

   Andrew Scharenberg

   Type

   Empty backbone

   Use

   Lentiviral and TALEN

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   May 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pShuttle-CMV-GFP H1promoter

   Plasmid

   #128050

   Purpose

   Adenoviral shuttle vector for shRNA expression from H1 promoter with GFP marker

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Adenoviral

   Expression

   Mammalian

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTALEN(Nd154C63) Ef1a

   Plasmid

   #50433

   Purpose

   pTAL plasmid compatible with the Golden Gate TALEN cloning system, for cloning of TALENs and expression in mammalian cells.

   Depositor

   Andrew Scharenberg

   Type

   Empty backbone

   Use

   Lentiviral and TALEN

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. FusionRed-FTR940-N-11

   Plasmid

   #62737

   Purpose

   Localization: Nuclear - Nucleolus, N1 Cloning Vector, Excitation:580, Emission:608

   Depositor

   Michael Davidson

   Insert

   FTR940
   

   Tags

   FusionRed

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   April 1, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pDEST-Opa1

   Plasmid

   #191961

   Purpose

   Gateway Destination Vector, containing OPA1 coding sequence, for Gateway Cloning Technology

   Depositor

   Luca Scorrano

   Insert

   Opa1
   

   Use

   Synthetic Biology

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR-Myt1NB580

   Plasmid

   #22740

   Depositor

   Lynn Hudson

   Insert

   Myelin Transcription Factor 1 (Myt1 Mouse)
   

   Use

   Pcr cloning vector

   Available Since

   Dec. 18, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. Dual-sgRNA_hU6-mU6

   Plasmid

   #154194

   Purpose

   Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVITRO1-M80-F2-IgG1/κ

    Plasmid

    #50383

    Purpose

    Expresses human IgG1/κ antibody isotype with unknown specificity. Useful vector for cloning of human antibodies with kappa light chain

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    human Gamma 1 heavy chain expression cassette with unknown specificity
    human Kappa light chain expression cassette with unknown specificity
    

    Expression

    Mammalian

    Promoter

    Mouse Elongation Factor 1 Alpha and Rat Elongatio…

    Available Since

    Jan. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB_Tet-Off_Flex_DEST_EFS_mODC_tTA (JDW 936)

    Plasmid

    #229828

    Purpose

    A Piggybac-compatible, cre-dependent, Tet-off destination vector with a downstream WPRE. EFS driving destabilized tTA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGEN-WPRE-bGH-DEST (JDW 1309)

    Plasmid

    #229815

    Purpose

    A CAGGS driven, gateway compatible destination vector that contains a WPRE downstream of the destination cassette to stabilize transcripts

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONRG_P4-P1R:MUM4_0.3Pro_35S

    Plasmid

    #128558

    Purpose

    Gateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing a 307 bp MUM4 (At1g53500) promoter fragment fused to the 54 bp 35S minimal promoter sequence, for use in Three-way Gateway cloning

    Depositor

    George Haughn

    Article

    Dean et al Plant Mol Biol. 2017 Sep;95(1-2):33-50.

    Type

    Empty backbone

    Use

    Gateway promoter entry clone

    Promoter

    MUM4 core promoter with 35S enhancer

    Available Since

    Sept. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. P1_Firre_1.49_pDONR221

    Plasmid

    #195204

    Purpose

    Plasmid with pDONR221 backbone containing Firre 1.49 clone. Kanamycin resistance. Gateway cloning system.

    Depositor

    John Rinn

    Article

    Hacisuleyman et al Nat Struct Mol Biol. 2014 Feb;21(2):198-

    Insert

    mFirre 1.49 Clone
    

    Use

    Gateway cloning intermediate

    Mutation

    Only exons

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pB-TetOn-DEST-EFS-mODC-rtTA-IRES-NEO (JDW 931)

    Plasmid

    #242578

    Purpose

    PiggyBac transposon flanked, Tet-on, gateway destination vector.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    attR1-CmR-ccdB-attR2
    

    Expression

    Mammalian

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGEN-DV-IRES-myr-mKate (JDW 494)

    Plasmid

    #242575

    Purpose

    Gateway destination vector with a CAGGS promoter in the backbone as well as an IRES-myr-mKate to label cell membranes.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    attR1-CmR-ccdB-attR2
    

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pB-2x-lns-DEST (JDW 1206)

    Plasmid

    #229818

    Purpose

    A PiggyBac destination vector compatible with gateway with 2 core cHS4 insulators by each ITR site

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDestTol2pA-2xIns-fli1ep-zCreI-BFP (JDW 1218)

    Plasmid

    #229819

    Purpose

    A gateway compatible Tol2 destination vector containing the fli1a promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tol2 destination vector

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBeta-MHC-promoter DV (JDW 26)

    Plasmid

    #229835

    Purpose

    A gateway compatible destination vector containing the murine beta-MHC promoter for embryonic and postnatal cardiomyocyte expression.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXMLC2-DEST (JDW 7)

    Plasmid

    #229833

    Purpose

    A gateway compatible destination vector containing the Xenopous myosin light chain 2 promoter for embryonic and adult cardiomyocyte expression.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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