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167,212 results

Showing: 4561 - 4600 of 167212 results

  6. pKEK3235

    Plasmid
    #234107
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    sfGFP
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    S30R/Y39N/N105T/Y145F/I171V/A206V
    Promoter
    pJ23100-BsaI
    Available Since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    234107-plasmid-map-sequence-id-467990

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  7. pKEK3233

    Plasmid
    #234105
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    YukonOFP
    Expression
    Bacterial
    Promoter
    pJ23100-BsaI
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234105-plasmid-map-sequence-id-464918

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  10. RFP-PPPPPPAAAA-gs

    Plasmid
    #228092
    Purpose
    Mammalian expression of 10 coiled coil segments in PPPPPPAAAA arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cells
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, PPPPPPAAAA
    Expression
    Mammalian
    Available Since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    228092-plasmid-map-sequence-id-451390

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  14. hzHDAC10

    Plasmid
    #225542
    Purpose
    Expresses histone deacetylase 10 (HDAC10) humanized catalytic domain, also known as hzHDAC10, in Escherichia coli cells.
    Depositor
    David Christianson
    Article
    Herbst-Gervasoni et al ACS Chem Biol. 2020 Aug 21;15(8):2154-21
    Insert
    Histone Deacetylase 10 humanized catalytic domain (hdac10 Zebrafish)
    Tags
    His6-MBP tag with cleavable TEV site
    Expression
    Bacterial
    Mutation
    Mutated A24E and D94A
    Promoter
    T7
    Available Since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225542-plasmid-map-sequence-id-448447

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  16. pBH504

    Plasmid
    #215782
    Purpose
    NBU1 backbone with constitutive dCas9, LacI(YQR), P(O1)_sgRNA targeting B. theta amylopectin SusC gene
    Depositor
    Corey Wilson
    Article
    Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:
    Inserts
    LacI(YQR)
    sgRNA-ribozyme cassette
    dCas9
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Bt1311, P(O1), and P1
    Available Since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    215782-plasmid-map-sequence-id-430549

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  18. CSII-CAG-EGFP

    Plasmid
    #214112
    Purpose
    Mammalian continuous expression
    Depositor
    Sho Yoshimatsu
    Insert
    EGFP
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available Since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    214112-plasmid-map-sequence-id-423582

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  32. pJBEI-6409

    Plasmid
    #47048
    Purpose
    BglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli
    Depositor
    Taek Soon Lee
    Article
    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1
    Insert
    Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
    Use
    Synthetic Biology; Bglbrick
    Expression
    Bacterial
    Mutation
    R364S mutation in MK
    Promoter
    PlacUV5
    Available Since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    47048-plasmid-map-sequence-id-67070

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  34. pDIRECT_22C

    Plasmid
    #91135
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Available Since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91135-plasmid-map-sequence-id-182261

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  35. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA

    Plasmid
    #154868
    Purpose
    Human synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherry
    Depositor
    Ulrik Gether
    Has Service
    AAV Retrograde and AAV8
    Insert
    hM3D(Gq)-mCherry
    Use
    AAV; Flp-frt
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    Human Synapsin-1
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    154868-plasmid-map-sequence-id-308827

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  37. pLenti.CAG.H2B-Dendra2.W

    Plasmid
    #51005
    Purpose
    Lentivector that expresses H2B-Dendra2 from internal CAG enhancer-promoter
    Depositor
    Rusty Lansford
    Insert
    H2B-Dendra2
    Use
    Lentiviral
    Tags
    histone2B
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51005-plasmid-map-sequence-id-490664

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  39. TFORF3550

    Plasmid
    #145026
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    mCherry
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    145026-plasmid-map-sequence-id-422981

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  40. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139090
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139090-plasmid-map-sequence-id-273886

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Showing: 4561 - 4600 of 167212 results