166,169 results

1. pAAV/mIba1.GFP.miR-9.T.miR-129-2-3p.T.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA

   Plasmid

   #226475

   Purpose

   Improved microglia-targeted transgene expression

   Depositor

   Hirokazu Hirai

   Article

   Aoki et al Cell Rep Methods. 2025 Jul 25:101116. do

   Inserts

   mIba1 promoter, 1.7-kb, microglia-specific promoter
   target sequences for miR-9x4 & miR-129-2-3px4 *2
   EGFP
   

   Use

   AAV

   Available Since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. POLG-pcDNA3.1

   Plasmid

   #191487

   Purpose

   Expresses human POLG gene on pcDNA3.1 backbone. POLG gene is cloned with BamHI and XbaI restriction sites.

   Depositor

   Hans Zempel

   Insert

   POLG (POLG Human)
   

   Tags

   V5 tag, 6X His

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEM047 [T7-assisted CROPseq sgRNA expression vector]

   Plasmid

   #224899

   Purpose

   Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA

   Depositor

   Thomas Norman

   Article

   Metzner et al Cell Syst. 2025 Jan 15;16(1):101161. doi

   Inserts

   eGFP
   LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ER-GCaMP6-150

   Plasmid

   #86918

   Purpose

   Fluorescent reporter for ER calcium signaling

   Depositor

   Timothy Ryan

   Article

   de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

   Insert

   ER-GCaMP6-150
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

   Promoter

   CAMKII

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AiP13563 - pAAV-AiE0170h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3563)

   Plasmid

   #224029

   Purpose

   AiE0170h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Sst+ neurons

   Depositor

   Allen Institute for Brain Science, Jonathan Ting

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Mutation

   NA

   Promoter

   minBG

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCK007 pME KalTa4

   Plasmid

   #195965

   Purpose

   Gateway pME Gal4 variant

   Depositor

   Alexa Burger, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   KalTA4 (Gal4)
   

   Use

   Other

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1_sfCherry2(1-10)

   Plasmid

   #82602

   Purpose

   Expresses sfCherry2(1-10) fusion in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   sfCherry2(1-10)
   

   Expression

   Mammalian

   Mutation

   changed Glu 118 to Gln, changed Thr 128 to Ile

   Promoter

   CMV

   Available Since

   Jan. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GFP scFv [N86/38]

   Plasmid

   #182095

   Purpose

   Mammalian Expression of GFP scFv. Derived from hybridoma N86/38.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting GFP
   

   Tags

   HA, His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mEmerald-MAP4-N-10

   Plasmid

   #54153

   Purpose

   Localization: Microtubules, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   MAP4 (MAP4 Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pSNAPf-ADRβ2 Control Plasmid

    Plasmid

    #101123

    Purpose

    Expression of SNAP-Tagged β2 Adrenergic Receptor in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    Beta-2-Adrenergic Receptor (ADRB2 Human)
    

    Tags

    SNAP-tag (SNAPf)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pMRXIP TMEM41B-GFP

    Plasmid

    #117414

    Purpose

    Expresses TMEM41B tagged with GFP in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

    Insert

    TMEM41B (TMEM41B Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PB-GFP-Gal8

    Plasmid

    #127191

    Purpose

    Piggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.

    Depositor

    Jordan Green

    Article

    Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

    Insert

    GFP-Gal8 (LGALS8 Human)
    

    Tags

    Gal8 is fused to the c-terminus of GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Rtn3S-GFP

    Plasmid

    #169035

    Purpose

    Express human Rtn3S in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

    Insert

    Rtn3S (RTN3 Human)
    

    Tags

    acGFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28-hXRCC1

    Plasmid

    #70759

    Purpose

    bacterial expression of human XRCC1

    Depositor

    Primo Schaer

    Article

    Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

    Insert

    XRCC1 (XRCC1 Human)
    

    Tags

    6-HIS

    Expression

    Bacterial

    Promoter

    T7/lac operon

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.4-GFP-TEV-B55

    Plasmid

    #217378

    Purpose

    expresses GFP tagged B55 in mammalian cells; tev cleavable

    Depositor

    Wolfgang Peti

    Article

    Padi et al Nature. 2024 Jan;625(7993):195-203. doi:

    Insert

    B55 (PPP2R2A Human)
    

    Tags

    EGFP TEV

    Expression

    Mammalian

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pQE-60NA-proinsulin-msGFP2

    Plasmid

    #160466

    Purpose

    Encodes a fusion of human proinsulin and the monomeric superfolder GFP2

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    proinsulin-msGFP2
    

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mCerulean N1

    Plasmid

    #27795

    Depositor

    Steven Vogel

    Article

    Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

    Insert

    mCerulean N1
    

    Expression

    Mammalian

    Available Since

    April 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV/SB10

    Plasmid

    #24551

    Depositor

    Perry Hackett

    Article

    Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

    Insert

    Sleeping Beauty
    

    Expression

    Bacterial

    Available Since

    Oct. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. N-Cadherin-EGFP

    Plasmid

    #18870

    Depositor

    Valeri Vasioukhin

    Article

    Nechiporuk et al Dev Cell. 2007 Sep . 13(3):338-50.

    Insert

    N-Cadherin (Cdh2 Mouse)
    

    Tags

    EGFP, Kanamycin, and N-Cadherin

    Expression

    Mammalian

    Available Since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. huntingtin_FL_NbioCflag

    Plasmid

    #194767

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells

    Depositor

    Cheryl Arrowsmith

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    Avi-tag (biotin acceptor peptide) and Flag-tag

    Expression

    Mammalian

    Mutation

    Q23 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    July 27, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. LifeAct-mNeonGreen

    Plasmid

    #98877

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    LifeAct
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti_Ef1a:PE2-P2A-hMLH1dn

    Plasmid

    #184444

    Purpose

    Single EF1a-driven PE2-P2A-hMLH1dn (Addgene #174828) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

    Depositor

    Bobby Koeleman

    Article

    Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and Ef1A

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSpdCas9-huTET1CD-T2A-GFP (PX458)

    Plasmid

    #129025

    Purpose

    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA cloning site
    

    Tags

    HA-Tag, NLS and T2A-EGFP

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLKO.1 Sox2 HM a

    Plasmid

    #26353

    Depositor

    Matthew Meyerson

    Article

    Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

    Insert

    Sox2 shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMRE131

    Plasmid

    #118485

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mTurquoise2
    

    Expression

    Bacterial

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Lamp1-RFP

    Plasmid

    #1817

    Purpose

    Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.

    Depositor

    Walther Mothes

    Article

    Sherer et al Traffic 2003 Nov;4(11):785-801.

    Insert

    lysosome associated membrane protein 1 (Lamp1 Rat)
    

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    D50E (not important for function of plasmid)

    Available Since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAVS1-P-CAG-GFP

    Plasmid

    #80491

    Purpose

    donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expression

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    CAG-GFP
    

    Use

    Donor vector (human)

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 4xNLS-pMJ915v2-sfGFP

    Plasmid

    #88921

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Inserts

    Cas9
    T7
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 476 p5E-mcs

    Plasmid

    #26029

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Type

    Empty backbone

    Use

    Entry vector

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNeonGreen-C1-Nir2-LNS2

    Plasmid

    #220086

    Purpose

    C-terminal PA binding domain of Nir2

    Depositor

    Gerry Hammond

    Article

    Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

    Insert

    PITPNM1 (PITPNM1 Human)
    

    Tags

    NeonGreen-GGSGGM

    Expression

    Mammalian

    Mutation

    amino acids 896-1216

    Promoter

    CMV

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Lenti-LucOS

    Plasmid

    #22777

    Depositor

    Tyler Jacks

    Insert

    OVA-SIY
    

    Use

    Cre/Lox and Lentiviral

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pet-15b-ultraID

    Plasmid

    #172879

    Purpose

    expresses ultraID in E. coli

    Depositor

    Julien Béthune

    Article

    Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

    Insert

    ultraID
    

    Tags

    myc

    Expression

    Bacterial

    Mutation

    fragment ([aa2-171]) of aquifex aeolicus BirA wit…

    Promoter

    T7

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHDM-Tat1b

    Plasmid

    #164442

    Purpose

    Encodes HIV-1 Tat accessory protein for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    HIV-1 Tat
    

    Expression

    Mammalian

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid

    #182653

    Purpose

    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Inserts

    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    

    Tags

    HA tag and nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    E55D and Y306A/Y384F (AF) double mutant of Methan…

    Promoter

    CMV and U6

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCRISPR::rpsL

    Plasmid

    #44505

    Purpose

    A crRNA expression plasmid specific to the rpsL allele.

    Depositor

    Luciano Marraffini

    Article

    Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    CRISPR::rpsL (rpsL )
    

    Use

    CRISPR; E.coli

    Available Since

    April 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. HcKCR2_mCherry_pcDNA3.1

    Plasmid

    #177337

    Purpose

    Expresses KCR2 in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

    Insert

    HcKCR2
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    mammalian codon-adapted

    Available Since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. All_in_one_CRISPR/Cas9_LacZ

    Plasmid

    #74293

    Purpose

    "All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screening

    Depositor

    Lynne Postovit

    Article

    Findlay et al PLoS One. 2016 Apr 18;11(4):e0153901. do

    Inserts

    LacZ-alpha
    Cas9
    mCherry
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Promoter

    Lac promoter, SV40, and T7 promoter

    Available Since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. 18065-M01-412

    Plasmid

    #225664

    Purpose

    Lentiviral expression of FLAG-tagged fluorescent proteins for immunohistochemical detection in FFPE tissue

    Depositor

    Dominic Esposito

    Article

    Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

    Inserts

    dsCopGFP
    delCD19 (CD19 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Mutation

    C-terminally truncated (aa 1-333 only)

    Promoter

    SORE6-mCMVp and WPRE-SV40p

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. mito-dendra2

    Plasmid

    #55796

    Purpose

    photo-activatable mitochondrial marker for expression in mammalian cells

    Depositor

    David Chan

    Article

    Pham et al Genesis. 2012 Nov;50(11):833-43. doi: 10

    Insert

    mito-Dendra2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRK5_mCherry_P2AT2A_GFP-nb-2xKS_FtoG

    Plasmid

    #238237

    Purpose

    For overexpression of mCherry_P2AT2A_GFP-nb-2xKS_FtoG

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry_P2AT2A_GFP-nb-2xKS_FtoG
    

    Expression

    Mammalian

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge