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Showing: 4561 - 4600 of 6325 results

  6. p5E-EFS (EF1a core) (JDW 1319)

    Plasmid
    #224474
    Purpose
    Gateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv 2024.07.13.603267
    Insert
    EFS promoter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    224474-plasmid-map-sequence-id-447208

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  18. pC1_oROS-HT

    Plasmid
    #216413
    Purpose
    Expresses the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.
    Depositor
    Andre Berndt
    Article
    Lee et al bioRxiv 2024.02.06.579232
    Insert
    oROS-HT
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216413-plasmid-map-sequence-id-435275

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  19. HCA3-DuET

    Plasmid
    #213309
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HCA3 (HCAR3 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213309-plasmid-map-sequence-id-430847

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  32. pPAP_scFv_V50A

    Plasmid
    #183734
    Purpose
    Expresses anti-lysozyme scFv antibody with V50A mutation on MFalpha signal
    Depositor
    Jun Ishii
    Article
    Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1
    Insert
    Anti-lysozyme scFv
    Use
    Tags
    His6-tag
    Expression
    Yeast
    Mutation
    V50A mutation in MFalpha signal
    Promoter
    AOX1 promoter
    Available since
    Feb. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    183734-plasmid-map-sequence-id-365753

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  33. Amber-free HIV-1 deltaRT

    Plasmid
    #213007
    Purpose
    An replication-incompetent amber-free HIV-1 encodes full-length Env BG505 with RT-deleted Q23 as the backbone.
    Depositor
    Maolin Lu
    Article
    Ao et al Cell Chem Biol. 2024 Jan 8:S2451-9456(23
    Inserts
    HIV-1 Q23 ΔRT backbone (reverse transcriptase is deleted)
    Env BG505
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    213007-plasmid-map-sequence-id-420529

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Showing: 4561 - 4600 of 6325 results