We narrowed to 21,502 results for: SPR

Showing: 4561 - 4600 of 21502 results

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pSEVA451-Cpf1

Plasmid

#192362

Purpose

CRISPR-based vector for genome editing

Depositor

Juana María Navarro Llorens

Article

Baldanta et al Microb Cell Fact. 2022 May 28;21(1):103.

Insert

Cas12a, gRNA scaffold

Use

CRISPR

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pYC1446

Plasmid

#158720

Purpose

Integrating plasmid expressing Sth1 Cas9 and the cognate sgRNA

Depositor

Yi-Cheng Sun

Article

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

Sth1 sgRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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SS-HA-Sun1L-KDEL

Plasmid

#187016

Purpose

Disruption of LINC Complex

Depositor

Jan Lammerding

Article

Lombardi et al J Biol Chem. 2011 Jul 29;286(30):26743-5

Insert

SS-HA-Sun1L-KDEL

Use

Lentiviral

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RTP1S

Plasmid

#149364

Purpose

Expresses RTP1S in mammalian cells.

Depositor

Takeshi Imai

Article

Inagaki et al Cell Rep. 2020 Jun 30;31(13):107814. doi

Insert

RTP1S (Rtp1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pRU325

Plasmid

#167676

Purpose

Intermediate vector for eight gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pFnCpf1GG

Plasmid

#131010

Purpose

Backbone plasmid for generating CRISPR arrays for FnCas12a. Contains a GFP-dropout cassette and a direct repeat.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Inserts

direct repeat of FnCas12a
GFP expression cassette

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pADH118

Plasmid

#90983

Purpose

NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.

Depositor

Aaron Hernday

Article

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Insert

NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, C. albicans LEU2 2 of 2

Use

CRISPR

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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AAV1 for Split-PE, SpCas9 nickase (PKC1001)

Plasmid

#190111

Purpose

AAV1 for expression of nickase SpCas9(H840A) for Split-PE use

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

nSpCas9

Use

AAV

Tags

bpNLS

Mutation

H840A

Promoter

EF1a

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Target-AIDmax

Plasmid

#139107

Purpose

Mammalian Target-AIDmax expression vector (pSI-626)

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Bi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pKEW189-MBP-TEV-AcrVA1

Plasmid

#115660

Purpose

Expresses AcrVA1 in bacteria via T7 RNAP, for purification

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA1

Tags

MBP-TEV

Expression

Bacterial

Promoter

T7 RNAP

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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HPRT2b-pGEM

Plasmid

#69539

Purpose

gRNA targeting human HPRT gene

Depositor

Hong Yan

Article

Liao et al Nucleic Acids Res. 2015 Jun 29. pii: gkv

Insert

HPRT gRNA2

Expression

Mammalian

Available Since

Sept. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQAT57

Plasmid

#223429

Purpose

T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

2x35s-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCas-Cas12m

Plasmid

#192274

Purpose

Encodes MmCas12m under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmCas12

Use

CRISPR

Expression

Bacterial

Promoter

PJ23108

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pUC57-Amp_CRISPIE donor B1

Plasmid

#172842

Purpose

CRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-0) encoding mEGFP

Use

CRISPR

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-H3FAID

Plasmid

#127884

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter H3FAID tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-H3F-AID

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pChimera

Plasmid

#61476

Purpose

Vector for conventional cloning that contains AtU6-26 promoter and sgRNA backbone

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

U6-26:sgRNA

Use

CRISPR

Expression

Plant

Promoter

U6-26

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pTU422

Plasmid

#178982

Purpose

13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)

Depositor

Stan Brouns

Article

van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

Inserts

gRAMP
CRISPR array

Tags

Strep-Tag II SUMO

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pdCas9+sgRNA expression vector precursor

Plasmid

#171798

Purpose

PICASSO: Library expression vector precursor for paired dCas9+sgRNA expression

Depositor

Stephen Elledge

Article

Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

pLtetO

Available Since

Aug. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pZmU3-gRNA

Plasmid

#53061

Purpose

Transcription of guide RNA (gRNA) in maize cells

Depositor

Caixia Gao

Article

Liang et al J Genet Genomics. 2014 Feb 20;41(2):63-8

Insert

zmU3 promoter - gRNA

Use

CRISPR; Plant expression

Promoter

maize U3

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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dPspCas13b-mutFTO

Plasmid

#223697

Purpose

Expressing fusion protein of dPspCas13b and inactive mutant of human FTO

Depositor

Chuan He

Article

Wei et al Science. 2022 May 27;376(6596):968-973.

Insert

dPspCas13b-GGS-mutFTO (FTO Human)

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pDualIN-SMN2

Plasmid

#214430

Purpose

Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7

Depositor

Chaolin Zhang

Article

Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

Insert

SMN2 EXON 7 (SMN2 Human)

Expression

Mammalian

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-U6-sgTel-CMV-puro-P2A-mCherry-TRF1

Plasmid

#169449

Purpose

expression of TRF1-mcherrry and sgRNA targeting telomeres

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

mCherry-TRF1

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ5079_pHR_PGK_sfGFP_CoV-F1

Plasmid

#155303

Purpose

SARS-CoV-2 fluorescent reporter 1

Depositor

Stanley Qi

Article

Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

Insert

Superfolder GFP fused with SARS-CoV-F1 (ORF1ab Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBZcsx27

Plasmid

#89900

Purpose

Expresses csx27 from B. zoohelcum ATCC 43767 in bacteria. Expression driven from the Lac promoter

Depositor

Feng Zhang

Article

Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

Insert

csx27

Expression

Bacterial

Promoter

Lac

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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LbCas12a-crRNA

Plasmid

#213753

Purpose

For circular RNA-mediated prime editor using LbCas12a-crRNA in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

LbCas12a-crRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_eA3A-nCas9

Plasmid

#163535

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

eA3A-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSH824-ssr

Plasmid

#198027

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

T0 terminator - Apr resistance cassette

Expression

Bacterial

Promoter

AprR promoter

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pG4

Plasmid

#162605

Purpose

Edit Ade2 gene in yeast

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Expression

Yeast

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pDONOR-tagBFP-PSM-EGFP

Plasmid

#100603

Purpose

Donor scaffold with EGFP positive selection module, and tagBFP negative selection module

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Type

Empty backbone

Use

Synthetic Biology

Tags

EGFP-T2A-Puro and tagBFP

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)

Plasmid

#225755

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detection

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pSAG1::CAS9-U6::sg290860-6

Plasmid

#59855

Purpose

Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.

Depositor

David Sibley

Article

Behnke et al Eukaryot Cell. 2014 Dec 5. pii: EC.00229

Insert

CRISPR sg290860-6

Use

CRISPR; ; toxoplasma gondii

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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Level 1 P4 TaU6 guide acceptor

Plasmid

#165600

Purpose

GoldenGate (MoClo) Level 1 Position 4 TaU6 guide acceptor plasmid

Depositor

Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

Article

Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

Insert

TaU6 promoter::LacZ::sgRNA scaffold

Use

CRISPR and Synthetic Biology; Sgrna acceptor with…

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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Level 1 P3 TaU6 guide acceptor

Plasmid

#165599

Purpose

GoldenGate (MoClo) Level 1 Position 3 TaU6 guide acceptor plasmid

Depositor

Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

Article

Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

Insert

TaU6 promoter::LacZ::sgRNA scaffold

Use

CRISPR and Synthetic Biology; Sgrna acceptor with…

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Ezh2FL

Plasmid

#235575

Purpose

Dox-inducible expression of epigenetic effector Ezh2 fused with scFV to programme H3K27me3

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Ezh2 (Ezh2 Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas3

Plasmid

#178881

Purpose

Expression of human codon-optimized NlaCas3 with C terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas3c-bGH polyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pUBC_V5_MS2-mVenus

Plasmid

#107606

Purpose

Transient expression of humanized MS2 (V75E/A81G), fused to mVenus in mammalian cells, under a hUBC promoter, V5 tag

Depositor

John Rinn

Article

Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

Insert

MS2-mVenus

Use

Synthetic Biology

Tags

V5 and mVenus

Mutation

MS2: V75E/A81G

Promoter

human UBC

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pDNR-SpCas9-Cdt1

Plasmid

#80426

Purpose

Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.

Depositor

Sara Howden, Melissa Little

Article

Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

Insert

SpCas9-Cdt1

Use

CRISPR

Tags

Cdt1

Mutation

codon optimized for human

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57-Amp_CRISPIE donor B5

Plasmid

#172846

Purpose

CRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP

Use

CRISPR

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pgRNA-non-target

Plasmid

#129465

Purpose

Derived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.

Depositor

Silvia Cardona

Article

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

Non-target/random gRNA

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 (SpeI)

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK Cas9-2A-Citrine

Plasmid

#92393

Purpose

Enhancer/reporter plasmid for tissue-specific expression of Cas9 with 2A-Citrine reporter. Contains BsmBI-flanked LacZ cloning cassette for rapid GoldenGate-based cloning of specific enhancers.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

Cas9 2A Citrine

Use

CRISPR

Expression

Mammalian

Promoter

thymidine kinase promoter

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 4561 - 4600 of 21502 results

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We narrowed to 21,502 results for: SPR