We narrowed to 13,488 results for: cas9 genes

1. MiniCoopR 2xU6:gRNA pten, mitfa:Cas9

   Plasmid

   #118845

   Purpose

   Targets ptena and ptenb and expresses zebrafish mitfa specifically in zebrafish melanocytes

   Depositor

   Leonard Zon

   Article

   Ablain et al Science. 2018 Nov 1. pii: science.aau650

   Insert

   ptena and ptenb (ptena, ptenb Zebrafish)
   

   Use

   CRISPR; Tol2

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. XClone-Cas9-Cronus-T2A-BFP

   Plasmid

   #141209

   Purpose

   Tunable and temporal expression control of Cas9

   Depositor

   Rory Johnson

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

   Plasmid

   #171089

   Purpose

   CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

   Inserts

   Decoy sgRNA
   dCas9
   Chloramphenicol acetyltransferase
   

   Use

   CRISPR; Toxoplasma gondii expression

   Tags

   3X FLAG and Nuclear Localization Signal

   Expression

   Mutation

   D10A, H840A

   Promoter

   TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2T-CMV-evoCjCas9-eAID-BlastR

   Plasmid

   #194062

   Purpose

   Mammalian expression of evoCjCas9 eAID

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-eAID
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-pMecp2-dSaCas9-pU6-sgRNA

   Plasmid

   #159174

   Purpose

   Vector B encodes pAAV-pMecp2-dSaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of dSaCas9-sgRNA in neurons

   Depositor

   Chung Tin

   Article

   Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

   Insert

   dSaCas9
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pMecp2

   Available since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-TREX1 g2 Cas9-T2A-mCherry

   Plasmid

   #164251

   Purpose

   Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   sgTREX1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJEP305-pAAV-EFS-dSaCas9-KRAB-pA

   Plasmid

   #113681

   Purpose

   A EFS driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   de-catalyzed SaCas9
   

   Use

   AAV and CRISPR

   Tags

   KRAB and NLS

   Expression

   Mutation

   Promoter

   Available since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUt-mPF-BACH1 Cas9-Resistant_AmpR

   Plasmid

   #199221

   Purpose

   LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Positive-Feedback circuit (mPF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 

   Depositor

   Gabor Balazsi

   Article

   Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

   Insert

   rtTA-Advanced::P2A::EGFP::BACH1 (BACH1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   silient mutation at nucleic acid 177 C changed to…

   Promoter

   tight TRE promoter

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCCL-PGK-SPdCas9-BFP-DNMT3A

   Plasmid

   #66819

   Purpose

   dCas9 fused to BFP and the human DNMT3A catalytic domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-BFP-DNMT3A, catalytic domain of DNMT3A
   

   Use

   Tags

   dSpCas9-BFP-DNMT3A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CMV-FLEX-SaCas9-U6-sgCnr1

    Plasmid

    #209196

    Purpose

    Mutagenesis of Cnr1

    Depositor

    Larry Zweifel

    Article

    Gunduz-Cinar et al Neuron. 2023 Jul 19:S0896-6273(23)00482-

    Insert

    Cnr1 (Cnr1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-EF1a-SPdCas9-DNMT3B-2A-Blast

    Plasmid

    #71217

    Purpose

    dCas9 fused to human DNMT3B catalytic domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-DNMT3B catalytic domain
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9

    Plasmid

    #107857

    Purpose

    To knock-out TDP43

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    gRNA against TARDBP (Exon 1) (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-eSpCas9-2A-GFP-sgRNA-HSD17B11

    Plasmid

    #161923

    Purpose

    To generate HSD17B11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against HSD17B11 exon 1.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    sgRNA targeting HSD17B11 exon 1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV_POLD2-APOBEC1-UdgX-NG-nCas9-UdgX

    Plasmid

    #163545

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    POLD2-APOBEC1-UdgX-NG-nCas9-UdgX
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6-TREX1 g1 Cas9-T2A-mCherry

    Plasmid

    #164250

    Purpose

    Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    sgTREX1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Eef1a-1955/-1>nls::Cas9::nls

    Plasmid

    #59987

    Purpose

    Eef1a (EF1alpha) promoter driving nls::Cas9::nls

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    nls::Cas9::nls
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Reverted mutations from dCas9 to wiltdtype

    Promoter

    Ciinte.Eef1a (EF1alpha) -1955/-1

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJEP303-pAAV-CMV-dSaCas9-VP64-pA

    Plasmid

    #113678

    Purpose

    A CMV driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    NLS and VP64

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. PET-Sniper SpCas9-NLS-6xHis

    Plasmid

    #207374

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    Sniper SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F539S, M763I, K890N

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCXLE-dCas9VPH-T2A-GFP-shP53

    Plasmid

    #102895

    Purpose

    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    dCas9VPH-T2A-GFP-shP53
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. SL2-sgTelo-MTSa/BFP/pdCas9-C1

    Plasmid

    #162760

    Purpose

    Expressing dCas9 and sgRNA containg MTSa targeting telomeres

    Depositor

    Antony K Chen

    Article

    Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

    Insert

    dCas9 and sgRNA(SL2-sgTelo-MTSa)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dCas9(nuclease deactivated Cas9)

    Promoter

    U6/CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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