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Plasmid#86934PurposepET LIC cloning vector for Biotin-His6-tev-yORF-mRuby2DepositorTypeEmpty backboneUseTags6xHis, AviTag (biotin), TEV cleavage site, and mR…ExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only
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pRSII325
Plasmid#35467DepositorTypeEmpty backboneUseYeast 2 micron vectorTagsExpressionMutationPromoterNoneAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
plp
Plasmid#22310DepositorTypeEmpty backboneUseIntegrating plasmidTagsExpressionYeastMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pgRGFP
Plasmid#82695PurposeExpresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterPGK, U6Available sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTX179
Plasmid#89261PurposeExpress STU (Single Transcriptional Unit) Cas9 nickase with Maize Ubiquitin1 promoter in plantsDepositorTypeEmpty backboneUseCRISPRTagsSV40 NLSExpressionPlantMutationPromoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMTU-DO-HIS-C4
Plasmid#160340PurposeHigh-copy expression vector for K. marxianus, histidine selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET mRuby2 LIC cloning vector (u-mRuby2)
Plasmid#86932PurposepET LIC cloning vector for yORF-mRuby2DepositorTypeEmpty backboneUseTagsmRuby2ExpressionBacterialMutationPromoterT7Available sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAT.000
Plasmid#119559PurposeSelf replication Level T acceptor vector with LacZ compatible blue/white screening. Modified BioBrick vector, RSF1010-derived ori.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA KanR neo
Plasmid#167909PurposeLentiviral vector for expressing U6 sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG426GPD-EYFP-ccdB
Plasmid#14348DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G4-Ph
Plasmid#46820PurposeTEF1 and HXT7 promoter controlled expression cassettes with phleomycin resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and HXT7Available sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMINTC3GH (Hyg)
Plasmid#110077PurposeAnalogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV106
Plasmid#63196PurposepRS406 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDRf1-4CL5-DsRed (JBEI-15461)
Plasmid#87935PurposeCloning vector for co-expressing a gene with 4CL5 in S.cerevisiaeDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG425GPD-EYFP-ccdB
Plasmid#14346DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
52 pLIB_3C_C_STREP
Plasmid#160300PurposeC-term 3C cleavable 2xStrepII tag for insect cell expressionDepositorTypeEmpty backboneUseTags2xStrepIIExpressionInsectMutationPromoterpolyhydrinAvailable sinceNov. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 mRuby2 LIC cloning vector (6H)
Plasmid#86965PurposeEmpty LIC vector; adds an mRuby2 gene to the C-terminus of your protein of interest.DepositorTypeEmpty backboneUseTagsmRuby2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2+/N-SNAPf
Plasmid#184415Purposevector backbone to clone insert downstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesisDepositorTypeEmpty backboneUseFor mrna synthesisTagsSNAPf-tagExpressionMutationCodon optimized for expression in Xenopus laevis …PromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2+/C-SNAPf
Plasmid#184416Purposevector backbone to clone insert upstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesisDepositorTypeEmpty backboneUseFor mrna synthesisTagsSNAPf-tagExpressionMutationCodon optimized for expression in Xenopus laevis …PromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA
Plasmid#186375PurposeDestination vector for the expression of a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGAT2
Plasmid#112588PurposeUsed for expression of protein in E. coli as Thrombin cleavable N-terminal 6His-tag-GST fusionsDepositorTypeEmpty backboneUseTags6His-GSTExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOSV807
Plasmid#126600Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pASR130
Plasmid#122638PurposeBglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae (pPEPZ Backbone)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC155-pCR8-sgExpression
Plasmid#49045PurposeU6-driven sgRNA expressing cassette on a gateway donor vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pIB167
Plasmid#90190PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pspac promoterDepositorTypeEmpty backboneUseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBRIT TAP
Plasmid#17520DepositorTypeEmpty backboneUseCre/Lox and RetroviralTagsTAPExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRiboI SapNde
Plasmid#54490PurposeTheophylline-inducible expression in single-copy integrating vectorDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterhsp60Available sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMEXC3GH (Hyg)
Plasmid#110082PurposeAnalogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMGE-T7
Plasmid#153021PurposeSingle-plasmid multi-gene expression system. Each genes under the control of a T7 and the lacO promoter sequences.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBCN22-R4R3
Plasmid#26302DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceDec. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSEVAT7Z1A
Plasmid#58300PurposeGeneGuard vector cassette: thyA, ColE2 COR, Zeta toxin; with ampR as cargo.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEL066
Plasmid#124897PurposeFor genome editing in plant by SpCas9 variant(SpCas9-NG) (D10A)-PmCDA1-UGI base editorDepositorTypeEmpty backboneUseCRISPRTags3xFlagExpressionPlantMutationPromoterAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pR6K-GFP-RFP
Plasmid#199652PurposeR6K backbone with Golden Gate (GG) cloning sitesDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterJ23119 promoterAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRATIO1112
Plasmid#141066PurposeGateway cloning compatible dual fluorescent ratiometric binary vectorDepositorTypeEmpty backboneUseTagsmScarlet-I and mVenusExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133C2.0
Plasmid#99893PurposeGolden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU6Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-BI16-FLAG-3C-ORF1_mClover3-TEV-ORF2
Plasmid#105801PurposeConcomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C or enterokinase; second protein expressed with mClover3, cleavable by TEV protease; tags positions: N-termini.DepositorTypeEmpty backboneUseFlp-in competentTagsORF1: FLAG-3C; ORF2: mClover3-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRC1765
Plasmid#141346PurposeMini plasmid containing minimal sequences for amplification in bacteria and two BbvCI nicking sites for introduction of modified DNA through annealing and ligation (e.g. mismatch, DNA lesions, flap).DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFloat.T7_v1
Plasmid#133070PurposeEntry vector for cloning genes under a T7 promoter for expression in bacteria. Version 1.0.DepositorTypeEmpty backboneUseEntry vectorTags3C-His6 and His6-3CExpressionBacterialMutationPromoterT7Available sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
plex_307-EYFP-ccdB-blasticidin
Plasmid#201131PurposepLEX_307 plasmid with N-terminal EYFP tag and blasticidin markerDepositorTypeEmpty backboneUseTagsEYFPExpressionMammalianMutationPromoterEF-1αAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only