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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 13,007 results for: cdna

Showing: 4641 - 4680 of 13007 results

  7. pcDNA3.1(+)Gα15-244-NLuc

    Plasmid
    #207154
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA15 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Depositor
    Dominique Schols, Tom Van Loy
    Article
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Insert
    GNA15 (GNA15 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLuc inserted at position E(244)N
    Promoter
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207154-plasmid-map-sequence-id-410274

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  11. Cav2.2 HA E1658A (EIVA) pcDNA3

    Plasmid
    #206114
    Purpose
    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658A mutation in the P loop of Domain IV which reduces trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E1658A
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206114-plasmid-map-sequence-id-415366

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  15. IRE1b(K536A).9E10.pCDNA3

    Plasmid
    #21881
    Depositor
    David Ron
    Insert
    IRE1b (Ern2 Mouse)
    Use
    Tags
    Myc (9E10)
    Expression
    Mammalian
    Mutation
    K536A
    Promoter
    Available since
    Nov. 10, 2009
    Availability
    Academic Institutions and Nonprofits only
    21881-plasmid-map-sequence-id-10062

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  22. pcDNA3-FLAG-LplA(AAG)

    Plasmid
    #61307
    Purpose
    Contains FLAG epitope for immunofluorescence detection of resorufin ligase for mammalian cell expression
    Depositor
    Alice Ting
    Article
    Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11
    Insert
    E. coli lipoic acid ligase
    Use
    Tags
    6xHis, Flag, T7 tag, and Xpress epitope
    Expression
    Mammalian
    Mutation
    E20A, F147A, H149G
    Promoter
    CMV
    Available since
    March 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61307-plasmid-map-sequence-id-99628

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  24. pcDNA3-PKCsub-YPet-NES

    Plasmid
    #138207
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKCsub-YPet-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    138207-plasmid-map-sequence-id-307410

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  27. pcDNA3-HCMVgO-8his (TB40/E)

    Plasmid
    #136453
    Purpose
    Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) with an 8his tag
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    envelope glycoprotein O (UL74 Human betaherpesvirus 5)
    Use
    Tags
    Linker and 8his tag (GGSGHHHHHHHH, includes BamHI…
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136453-plasmid-map-sequence-id-271682

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  28. pcDNA3-HCMVgO-8his (Merlin)

    Plasmid
    #136454
    Purpose
    Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) with an 8his tag
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    glycoprotein O (UL74 Human betaherpesvirus 5)
    Use
    Tags
    Linker and 8his tag (GGSGHHHHHHHH, includes BamHI…
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136454-plasmid-map-sequence-id-269878

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  29. pcDNA3-sACE2v2(732)-IgG1

    Plasmid
    #154105
    Purpose
    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (IgG1-Fc fusion)
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Use
    Tags
    Fc region of human IgG1
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease and neck domains …
    Promoter
    CMV
    Available since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154105-plasmid-map-sequence-id-304581

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  36. pcDNA3-FLAG-DDB1 (1140H)

    Plasmid
    #174849
    Purpose
    Expression of DDB1
    Depositor
    Helen Morrison
    Insert
    DDB1 (DDB1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    174849-plasmid-map-sequence-id-344539

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  38. pcDNA-zeo-hFJX1-myc

    Plasmid
    #46930
    Purpose
    expression of human FJX1-myc in mammalian cells
    Depositor
    Natasha Deane, R.D. Beauchamp
    Insert
    FJX1 (FJX1 Human)
    Use
    Tags
    myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 4641 - 4680 of 13007 results