We narrowed to 15,732 results for: LEA

1. HUHe variant click editor (CE1) - pCMV-T7-FiCV-nCas9-EcKlenow (JO1263)

   Plasmid

   #217787

   Purpose

   Variant CE1 construct with FiCV HUH endonuclease, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   FiCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. HUHe variant click editor (CE1) - pCMV-T7-GuCV-nCas9-EcKlenow (JO1279)

   Plasmid

   #217788

   Purpose

   Variant CE1 construct with GuCV HUH endonuclease, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   GuCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. HUHe variant click editor (CE1) - pCMV-T7-PCV1-nCas9-EcKlenow (JO1315)

   Plasmid

   #217789

   Purpose

   Variant CE1 construct with PCV1 HUH endonuclease, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV1-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDual

   Plasmid

   #207883

   Purpose

   Expresses thermosensitive variants of the homing endonucleases I-OnuI and I-GpeMI, each in a different open reading frame. Both were made thermosensitive by a fusion with the L212P VMA1 intein.

   Depositor

   David Edgell

   Article

   Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206

   Inserts

   Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
   Thermosensitive fusion of I-GpeMI and the L212P VMA1 intein.
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Leucine 212 changed to proline, leucine 434 in th…

   Available Since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSN-A112C-1A32

   Plasmid

   #191241

   Purpose

   Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.

   Depositor

   Alessandro Senes

   Article

   Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

   Insert

   nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
   

   Tags

   nuclease A from S. aureus fused to the poly-Leu T…

   Expression

   Bacterial

   Mutation

   Changed Alanine 112 to Cysteine in SN, and predic…

   Promoter

   T7 promoter

   Available Since

   Feb. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSN-A112C-1A31

   Plasmid

   #191242

   Purpose

   Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.

   Depositor

   Alessandro Senes

   Article

   Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

   Insert

   nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
   

   Tags

   nuclease A from S. aureus fused to the poly-Leu T…

   Expression

   Bacterial

   Mutation

   Changed Alanine 112 to Cysteine in SN, and predic…

   Promoter

   T7 promoter

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSN-A112C-CP8B1

   Plasmid

   #191240

   Purpose

   Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.

   Depositor

   Alessandro Senes

   Article

   Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

   Insert

   nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
   

   Tags

   nuclease A from S. aureus fused to the poly-Leu T…

   Expression

   Bacterial

   Mutation

   Changed Alanine 112 to Cysteine in SN, and predic…

   Promoter

   T7 promoter

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR2.1_HBB_A

   Plasmid

   #87848

   Purpose

   pCR2.1-based plasmid holding a 328-bp fragment containing the exon-1-exon-2 splice border of aberrant HBB[IVSI-110(G>A)] human β-globin mRNA

   Depositor

   Marina Kleanthous

   Article

   Patsali et al Int J Mol Sci. 2020 Sep 11;21(18). pii:

   Insert

   Homo sapiens hemoglobin subunit beta (HBB), Aberrant cDNA fragment (Exon1/19nt mispliced Intron1 + Exon2) (HBB Human)
   

   Expression

   Bacterial

   Mutation

   HBB(IVSI-110) β-thalassaemia misplicing mutation …

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMIG-hNFATc2/C(1-686)AVITEV

   Plasmid

   #74054

   Purpose

   retroviral expression plasmid for human NFATc2/C (without C-terminal region) with C-terminal BirA-biotinylation signal and TEV celavage site

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   human NFATc2, isoform C (NFATC2 Human, AS 1-686 (N terminus deleted))
   

   Use

   Retroviral

   Tags

   AVI-TEV

   Expression

   Mammalian

   Mutation

   silent mutation A1723C in the sequence of NM_1730…

   Promoter

   pMSCV-LTRs

   Available Since

   June 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid

    #182653

    Purpose

    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Inserts

    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    

    Tags

    HA tag and nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    E55D and Y306A/Y384F (AF) double mutant of Methan…

    Promoter

    CMV and U6

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a_His-TEV-EiCsm6

    Plasmid

    #172487

    Purpose

    Bacterial expression of codon-optimized His-EiCsm6 with a TEV protease cleavage site.

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

    Insert

    Enterococcus italicus Csm6 (codon optimized)
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7 promoter and lac operator

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pK27Sumo_His-SUMO-nsp10-nsp14 fusion (SARS-CoV-2)

    Plasmid

    #169161

    Purpose

    To express nsp10-nsp14 fusion protein in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    14His-SUMO-nsp10-GGSGGS-nsp14 (ORF1ab SARS-CoV-2, Synthetic)
    

    Tags

    14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1_hHNF4alpha

    Plasmid

    #206878

    Purpose

    The ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the Bam HI/XbaI sites of the pcDNA3.1(+), the mammalian expression vector.

    Depositor

    Catharine Ross, Reza Zolfaghari

    Article

    Zolfaghari et al J Cell Biochem. 2014 Oct;115(10):1740-51

    Insert

    Human hepatocyte nuclear factor 4 alpha (HNF4A Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDisplay-iSeroSnFR

    Plasmid

    #128484

    Purpose

    Fluorescent reporter for serotonin (mammalian expression, membrane localization tag)

    Depositor

    Lin Tian

    Article

    Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

    Insert

    iSeroSnFR
    

    Tags

    Ig-kappa leader, Myc, PDGFR + enhanced ER export,…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

    Plasmid

    #85756

    Purpose

    All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).

    Depositor

    Lawrence Stanton

    Article

    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

    Inserts

    Csy4-T2A-SpRFN-P2A-GFP
    gRNA
    

    Use

    CRISPR

    Tags

    Csy4, Csy4 recognition sequence, FokI fusion via …

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CMV and U6

    Available Since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. His-ZZ-TEV-SNAPf-DYNC2H1

    Plasmid

    #132524

    Purpose

    Full length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragments

    Depositor

    Andrew Carter

    Article

    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

    Insert

    DYNC2H1 (DYNC2H1 Human)
    

    Tags

    8x His, Linker-TEV-linker-TEV, Precission cleavag…

    Expression

    Insect

    Mutation

    Codon optimised for Sf9 expression

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHA TST 3C SMO EABR

    Plasmid

    #234989

    Purpose

    For production of Extracellular Vesicles (EVs), with the receptor Smoothened (Smo), Twin-Strep-tag (TST), and an HA tag at its N-terminus on their surface

    Depositor

    Daniel Leahy, Monica Gonzalez-Magaldi

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    SMO-EABR (SMO Human)
    

    Tags

    HA, Twin-Strep-Tag, HRV 3C site

    Expression

    Mammalian

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP

    Plasmid

    #112677

    Purpose

    An AAV vector that expresses a Cre-dependent nuclear-localized Red to Green Fluorescent protein

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Nuclear-localized floxed-mCherry EGFP
    

    Use

    AAV

    Tags

    Nuclear localization signal

    Promoter

    EF1a

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-IDO2-p2a-eGFP

    Plasmid

    #187027

    Purpose

    Expresses IDO2 and GFP (seperately) in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Maassen et al Free Radic Biol Med. 2022 Aug 1;188:287-

    Insert

    Indoleamine 2,3-dioxygenase 2 (IDO2 Human)
    

    Tags

    P2A-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CAG-iSeroSnFR-Nlgn

    Plasmid

    #128485

    Purpose

    Fluorescent reporter for serotonin (dendrite localization tag)

    Depositor

    Lin Tian

    Article

    Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

    Has Service

    AAV9

    Insert

    iSeroSnFR
    

    Use

    AAV

    Tags

    Full-length neuroligin, Ig-kappa leader, and cpsf…

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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