We narrowed to 29,740 results for: REP

1. pTol2-nbt-Electra1-P2A-mScarlet

   Plasmid

   #196113

   Purpose

   zebrafish expression of nbt-Electra1-P2A-mScarlet

   Depositor

   Reinhard Köster

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   nbt-Electra1-P2A-mScarlet
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-CT3G-ERP2-MG-IRES2-mNeonGreen

   Plasmid

   #175506

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G-mCMV

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSM_ser-2prom3p::myr-mCherry

   Plasmid

   #164223

   Purpose

   Expression of membrane-localized mCherry driven by the ser-2promo3 promoter. For use as a PVD neuron marker in C. elegans.

   Depositor

   Yan Zou

   Article

   Feng et al Cell Rep. 2020 Sep 8;32(10):108101. doi:

   Insert

   myr-mCherry
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   ser-2promo3

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pActin-Electra1

   Plasmid

   #184941

   Purpose

   beta-actin labelling with blue fluorescent protein Electra1

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   Electra1-beta-Actin
   

   Use

   Tags

   Electra1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-mRuby2-MACROD1_d77

   Plasmid

   #172588

   Purpose

   For transient expression of C-terminal mRuby2-tagged MACROD1del1-77

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   MACROD1 (MACROD1 Human)
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-76

   Promoter

   CMV

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. gRNAU6

   Plasmid

   #135963

   Purpose

   Contains tracrRNA-hU6 sequence for double sgRNA cloning

   Depositor

   Yongming Wang

   Article

   Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFUGW-ZipACR-EYFP

   Plasmid

   #88844

   Purpose

   Fast anion channelrhodopsin (current half-decay time 2-4 ms depending on voltage) for time-resolved inhibition of neuronal spiking

   Depositor

   John Spudich

   Article

   Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

   Insert

   ZipACR-NotI-EYFP
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mutation

   Promoter

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCytERM-Electra1

   Plasmid

   #179476

   Purpose

   ER Visualization (OSER assay) Expresses Electra1 in fusion with N-terminal part of p450

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   CytERM-Electra1
   

   Use

   Tags

   Electra1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDendra2-H2a

   Plasmid

   #157816

   Purpose

   Fluorescent reporter for histone H2A tracking

   Depositor

   Xin Chen

   Article

   Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

   Insert

   H2A (H2ac13 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCF287_EFS-TiCas9-P2A-Puro

    Plasmid

    #211646

    Purpose

    EFS-TiCas9-P2A-Puro. Tamoxifen-inducible SpyCas9.

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    Tamoxifen-inducible SpyCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDGB1alpha2_SulR

    Plasmid

    #186425

    Purpose

    transcriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter

    Depositor

    Lukas Fischer

    Article

    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

    Insert

    SulR
    

    Use

    Synthetic Biology; Binary vector for escherichia …

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSI-check2-Tab1-3'UTR

    Plasmid

    #97155

    Purpose

    For dual luciferase report assay

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Insert

    Tab1 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. OmEF1aCas9P2ApuroSB

    Plasmid

    #165484

    Purpose

    Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells

    Depositor

    Dietmar Kueltz

    Article

    Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10

    Insert

    Zebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Mutation

    Promoter

    Oreochromis mossambicus EF1 alpha promoter (OmEF1…

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CRY2-CreN

    Plasmid

    #75268

    Purpose

    The second half of the photo-activatable cre recombinase

    Depositor

    Michael Bruchas

    Article

    Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

    Insert

    second half of the cre recombinase fused to CRY2 (cryptochrome 2) that has absorbed blue light (Kennedy et al., 2010)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-CamK2-tTA

    Plasmid

    #210731

    Purpose

    AAV vector expressing tetracycline-controlled transactivator (tTA) under CamK2 promoter (for expressing tTA in glutamatergic neurons)

    Depositor

    Wei Xu

    Article

    Thompson et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    tetracycline-controlled transactivator (tTA)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CamK2

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAT024

    Plasmid

    #171636

    Purpose

    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-BFP
    mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGK:TRL-EFS:iRFP670

    Plasmid

    #68475

    Purpose

    Lentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and iRFP670

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    tTR-KRAB-2A-rtTA3-2A-Luciferase
    iRFP670
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and pGK

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-hSyn-EGFP-8X2C (LV GABA mAGNET)

    Plasmid

    #114212

    Purpose

    Lentiviral vector (control) that targets expression to GABAergic inhibitory neurons in rodent cortex

    Depositor

    Xue Han

    Article

    Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Lentiviral and Mouse Targeting

    Tags

    None

    Expression

    Mammalian

    Mutation

    None

    Promoter

    human synapsin (hSyn)

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. MAC_C_LMTK2

    Plasmid

    #187752

    Purpose

    MAC-tagged gene expression of human LMTK2

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    LMTK2 (LMTK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSFDuet-1_VNp15-DARPinOFF7

    Plasmid

    #182399

    Purpose

    Bacterial expression of Vesicle Nucleating peptide15-DARPinOFF7 fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp15-DARPinOFF7
    

    Use

    Tags

    Vesicle Nucleating peptide 15 (VNp15)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCMV MN (eGFP)

    Plasmid

    #89932

    Purpose

    Expresses the MN fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.

    Depositor

    Carl Novina

    Article

    Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

    Insert

    MN
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    M.SssI residues 2-272 fragment

    Promoter

    CMV

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. EcPPK1

    Plasmid

    #108850

    Purpose

    Expresses E. coli PPK1 in mammalian cell lines

    Depositor

    Michael Downey

    Article

    Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

    Insert

    Polyphosphate Kinase 1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAGGS-6017nes

    Plasmid

    #108655

    Purpose

    A PKA biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    hyBRET-PKA-EV
    

    Use

    Tags

    XFP

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGS

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCAGGS-6015nes

    Plasmid

    #108657

    Purpose

    A S6 Kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    hyBRET-S6K-EV
    

    Use

    Tags

    XFP

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGS

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. pENTR221-SMAD3

    Plasmid

    #79507

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human SMAD3 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    SMAD3 (SMAD3 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    no Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. pPotef-cdc3CB16-NatR

    Plasmid

    #86465

    Purpose

    Expresses an N-terminal Cdc3-mCherry fusion under the control of the constitutive Potef promotor. 16boxB stem-loops are integrated into the 3'UTR to visualize the mRNA.

    Depositor

    Michael Feldbrügge

    Article

    Baumann et al EMBO Rep. 2014 Jan;15(1):94-102. doi: 10

    Insert

    Cdc3
    

    Use

    Ustilago maydis expression

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Potef

    Available Since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. pCSIIbsr-6085x

    Plasmid

    #108653

    Purpose

    A tyrosine kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    PicchuEV
    

    Use

    Lentiviral

    Tags

    XFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAT-mCherry2-G

    Plasmid

    #164169

    Purpose

    Expresses mCherry2 under the control of TetO promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal, pAAV-TRE backbone

    Depositor

    Isei Tanida

    Article

    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

    Insert

    mCherry2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. TOPO rtTA3-2A-Cre

    Plasmid

    #68448

    Purpose

    TOPO construct expressing rtTA3-2A-Cre for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    rtTA3-2A-Cre
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in mice

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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30. MAC_C_FGFR2

    Plasmid

    #187751

    Purpose

    MAC-tagged gene expression of human FGFR2

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    FGFR2 (FGFR2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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