165,960 results

1. pLenSyn2-EGFP-­OMP25C

   Plasmid

   #71427

   Purpose

   Lentiviral expression of EGFP-OMP25C. For visualization of neuronal mitochondria.

   Depositor

   Henri Huttunen

   Article

   Kysenius et al PLoS One. 2014 Sep 5;9(9):e107129. doi:

   Insert

   OMP25 (172-­‐206) (Synj2bp Rat)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 172-206 only

   Promoter

   synapsin

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pCTCON2-GFP

   Plasmid

   #158130

   Purpose

   Single fluorescent protein reporter (GFP) for ncAA incorporation (wild-type construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   sfGFP
   

   Expression

   Yeast

   Promoter

   GAL1-10

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF2409

   Plasmid

   #144021

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOXF2 (FOXF2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. c-Fos

   Plasmid

   #187905

   Purpose

   Expresses c-Fos in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

   Insert

   cFos (FOS Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST-CMV mCherry-GFP-LC3B WT

   Plasmid

   #123230

   Purpose

   Expresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. MAP4K3-GFP

   Plasmid

   #21731

   Depositor

   L. Miguel Martins

   Article

   Lam et al Proc Natl Acad Sci U S A. 2009 Jul 21;10

   Insert

   MAP4K3 (MAP4K3 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Aug. 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP

   Plasmid

   #102796

   Purpose

   Retrovirus for delivery of one sgRNA (empty, bbsI)

   Depositor

   Sarah Teichmann

   Article

   Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

   Insert

   U6 sgRNA
   

   Use

   CRISPR and Retroviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. mEGFP-TPX2-N-10

   Plasmid

   #56289

   Purpose

   Localization: Centrosomes, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Insert

   TPX2 (TPX2 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

9. Tet-on circRNA Expression Vector

   Plasmid

   #92351

   Purpose

   The vector can ef_ciently produce a circRNA (circmCherry) from a split EGFP gene after Dox treatment.

   Depositor

   Ling-Ling Chen

   Article

   Zhang et al Cell Rep. 2016 Apr 19;15(3):611-24. doi:

   Insert

   mCherry and split EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   pTet

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. psd44-iGFP-MFGE8-long

    Plasmid

    #46847

    Purpose

    expression of MFGE8 in mammalian cells

    Depositor

    Agnese Mariotti

    Article

    Carrascosa et al Oncogene. 2012 Mar 22;31(12):1521-32. do

    Insert

    MFGE8 (Mfge8 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    PGK promoter

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pET32a-hTGFa

    Plasmid

    #199236

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Transforming growth factor alpha, partial (TGFA Human)
    

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. JMJD2A

    Plasmid

    #38846

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    JMJD2A (Kdm4a Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

13. LCV2_mCherry

    Plasmid

    #155096

    Purpose

    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mCherry expression

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDV044

    Plasmid

    #191824

    Purpose

    Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the KmR locus. pCPC600 promoter. With terminator. Golden Gate compatible. Selection: in A. platensis: KmR; E. coli: AmpR

    Depositor

    Jim Roberts

    Article

    Tabakh et al STAR Protoc. 2023 Feb 8;4(1):102087. doi

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmScarlet3-Peroxi_C1

    Plasmid

    #189769

    Purpose

    Construct for labelling peroxisomes in mammalian cells with peroxisomal targeted mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3-Peroxi
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. LCV2_mClover3

    Plasmid

    #155095

    Purpose

    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mClover3 expression

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. ACTN2-pEGFP

    Plasmid

    #52669

    Purpose

    Overexpression of GFP-tagged α-actinin-2

    Depositor

    Johannes Hell

    Article

    Hall et al Neuron. 2013 May 8;78(3):483-97. doi: 10

    Insert

    actinin 2 (ACTN2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-TO-dCas9-3XGFP

    Plasmid

    #64107

    Purpose

    Expression of Sp dCas9-3XGFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp dCas9
    

    Use

    Lentiviral

    Tags

    3XsfGFP

    Expression

    Mammalian

    Promoter

    CMV-TO

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. VAMP4-SEP

    Plasmid

    #174406

    Purpose

    Reporter for VAMP4 exocytosis in mammalian cells

    Depositor

    David Perrais

    Article

    Bakr et al Cell Rep. 2021 Sep 7;36(10):109678. doi:

    Insert

    vesicle associated membrane protein 4 (VAMP4 Human)
    

    Tags

    superecliptic phulorin (mutated GFP)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHW522 (Prab-3::cGAL-C::let-858 3'UTR)

    Plasmid

    #107130

    Purpose

    cGAL-C driver under the control of C. elegans rab-3 promoter

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    NLS::gp41-1-C-intein::cGAL(AD)
    

    Expression

    Worm

    Promoter

    C. elegans rab-3 promoter

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pEGFP-C1-hFGD5

    Plasmid

    #50760

    Purpose

    expression of EGFP-tagged human FGD5

    Depositor

    Naoki Mochizuki

    Article

    Ando et al J Cell Biol. 2013 Sep 16;202(6):901-16.

    Insert

    FGD5 (FGD5 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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22. PiggyBac dCas9-VPR

    Plasmid

    #191269

    Purpose

    piggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Inserts

    VPR-dCas9-HA-tagBFP
    rtTA3-IRES-PURO
    

    Use

    CRISPR

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pH2B-emiRFP670

    Plasmid

    #136571

    Purpose

    Histone 2B labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    H2B-emiRFP670
    

    Tags

    emiRFP670

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. pBabe-Puro-IKBalpha-mut (super repressor)

    Plasmid

    #15291

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    inhibitor of Kappa B alpha (NFKBIA Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    S32A/S36A: “super-repressor” allele (resistant to…

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAAV-hsyn-GRAB_HA1mut

    Plasmid

    #190475

    Purpose

    Express the histamine-insensitive control sensor GRAB_HA1mut in neurons

    Depositor

    Yulong Li

    Article

    Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

    Insert

    Histamine-insensitive sensor GRAB_HA1mut (control sensor)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. MSCV-C 18E7

    Plasmid

    #37886

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    HPV 18 E7 (E7 HPV)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    PKG

    Available Since

    July 26, 2012

    Availability

    Academic Institutions and Nonprofits only

27. pMXs-c-Myc

    Plasmid

    #13375

    Purpose

    Retroviral expression of mouse c-Myc

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    myelocytomatosis oncogene (Myc Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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28. pENTR223-BALF4

    Plasmid

    #195835

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BALF4

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BALF4 (BALF4 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. pHaMDRwt

    Plasmid

    #10957

    Depositor

    Michael Gottesman

    Article

    Pastan et al Proc Natl Acad Sci U S A. 1988 Jun . 85(

    Insert

    MDR1 (ABCB1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GFP-Sec31a #861#640

    Plasmid

    #42123

    Depositor

    Ayano Satoh

    Article

    Koreishi et al PLoS One. 2013;8(1):e54382. doi: 10.1371

    Insert

    Sec31a (SEC31A Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2013

    Availability

    Academic Institutions and Nonprofits only