We narrowed to 1,090 results for: rbis

1. pCMV5-Flag-EPLIN beta delta domain 3

   Plasmid

   #40939

   Depositor

   Scott Maul, Elizabeth Luna

   Article

   Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

   Insert

   EPLIN beta delta domain 3 (LIMA1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deleted amino acids 266-309

   Promoter

   Available since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1 2xFLAG-2xSTREP-Keap1 (mouse)

   Plasmid

   #236421

   Purpose

   transient overexpression of KEAP1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

   Insert

   KEAP1 (Keap1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1 2xFLAG-2xSTREP-KEAP1 (human)

   Plasmid

   #236422

   Purpose

   transient overexpression of KEAP1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

   Insert

   KEAP1 (KEAP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3 FLAG FBXO31

   Plasmid

   #236429

   Purpose

   transient overexpression of FBXO31 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Duan et al Cell Rep. 2021 Oct 19;37(3):109870. doi:

   Insert

   FBXO31 (FBXO31 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ER-RspA-NFAST

   Plasmid

   #233595

   Purpose

   Expression of RspA-NFAST on the ER membrane

   Depositor

   Riccardo Filadi

   Article

   Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

   Insert

   RspA-NFAST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ER-long-RspA-NFAST

   Plasmid

   #233600

   Purpose

   Expression of RspA-NFAST on the ER membrane after a 55 aminoacid linker

   Depositor

   Riccardo Filadi

   Article

   Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

   Insert

   RspA-NFAST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti-CMV-GFP-Puro_TAL1-long

   Plasmid

   #210642

   Purpose

   Overexpression of TAL1-long

   Depositor

   Maayan Salton

   Article

   Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

   Insert

   TAL1-long (TAL1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EF1a-SRRD-V5-APEX2 (in pLEX_307)

   Plasmid

   #214921

   Purpose

   constitutive expression of SRRD fused to V5 and APEX2 - used for APEX2 proximity biotinylation

   Depositor

   Ophir Shalem

   Article

   Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

   Insert

   SRRD (SRRD Human)
   

   Use

   Lentiviral

   Tags

   V5-APEX2

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti-CMV-GFP-Puro_TAL1-short

   Plasmid

   #210641

   Purpose

   Overexpression of TAL1-short

   Depositor

   Maayan Salton

   Article

   Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

   Insert

   TAL1-short (TAL1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Tet-pLKO-puro-shTAL1

    Plasmid

    #210640

    Purpose

    Knockdown of TAL1 endogenous (3'UTR)

    Depositor

    Maayan Salton

    Article

    Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

    Insert

    TAL1 3' UTR gRNA (TAL1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLCHKO_PDPR_exon_deletion_2

    Plasmid

    #155066

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLCHKO_SFRS7_exon_deletion_1

    Plasmid

    #155069

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLCHKO_SFRS7_exon_deletion_2

    Plasmid

    #155070

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLCHKO_MDM4_exon_deletion_1

    Plasmid

    #155073

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLCHKO_MDM4_exon_deletion_2

    Plasmid

    #155074

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLCHKO_MDM4_exon_deletion_4

    Plasmid

    #155076

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLCHKO_Ptbp_ex8_1

    Plasmid

    #155081

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLCHKO_Ptbp_ex8_4

    Plasmid

    #155084

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLCHKO_SFRS7_exon_deletion_4

    Plasmid

    #155072

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLCHKO_Ptbp_ex8_2

    Plasmid

    #155082

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLCHKO_PDPR_exon_deletion_3

    Plasmid

    #155067

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLCHKO_PDPR_exon_deletion_4

    Plasmid

    #155068

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRC/CMV-TYK2(delta)TK-VSV

    Plasmid

    #139353

    Purpose

    Expression of TYK2 protein missing the tyrosine kinase (TK) domain (aa 1-895)

    Depositor

    Sandra Pellegrini

    Article

    Velazquez et al J Biol Chem. 1995 Feb 17;270(7):3327-34.

    Insert

    TYK2 cDNA expressing aa 1-895 (TYK2 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    V362F-deletion of aa 896-1187

    Promoter

    CMV

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1(+)-FLAG-RGS14

    Plasmid

    #236567

    Purpose

    Expresses Flag-RGS14 in mammalian cells

    Depositor

    John Hepler

    Article

    Friedman et al J Biol Chem. 2022 May;298(5):101836. doi

    Insert

    Homo sapiens regulator of G-protein signaling 14 (RGS14 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pnCS_SEPT6_SEPT7-TEV-Strep

    Plasmid

    #174499

    Purpose

    bacterial co-expression of human SEPT6 and of human SEPT7

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN6 (SEPTIN6 Human)
    SEPTIN7 (SEPTIN7 Human)
    

    Use

    Tags

    TEV-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-HA-CDK4

    Plasmid

    #172612

    Purpose

    Expresses HA-tagged CDK4 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti6.2_3xFLAG_V5_ mmDdx58_V5

    Plasmid

    #160108

    Purpose

    Expresses murine Ddx58 in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Ddx58 (Rigi Mouse)
    

    Use

    Lentiviral

    Tags

    3xFLAG, V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ER-mit RspA-splitFAST (lentiviral plasmid)

    Plasmid

    #233604

    Purpose

    Expression of ER-mit RspA-splitFAST by a single lentiviral plasmid encoding the two fragments

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    RspA-splitFAST
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV/SV40

    Available since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-CCND1

    Plasmid

    #172632

    Purpose

    Expresses untagged cyclin D1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pnEA-vH_His-TEV-SEPT2-msfGFP

    Plasmid

    #174492

    Purpose

    bacterial expression of human SEPT2 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Insert

    SEPTIN2 (SEPTIN2 Human)
    

    Use

    Tags

    His6-TEV and msfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLCHKO_intergenic-intergenic_2

    Plasmid

    #155078

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pnEA-vH_His-TEV-SEPT2_SEPT6

    Plasmid

    #174497

    Purpose

    bacterial co-expression of human SEPT2 and of human SEPT6

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN2 (SEPTIN2 Human)
    SEPTIN6 (SEPTIN6 Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMVtrunc Affimer6-msfGFPΔN12

    Plasmid

    #231550

    Purpose

    Mammalian expression of Affimer6 fused to N-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    Affimer6
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMVtrunc

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1-HA-CCND3

    Plasmid

    #172621

    Purpose

    Expresses HA-tagged cyclin D3 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. BICstim-Gag-dCAS9-VPR

    Plasmid

    #120922

    Purpose

    encodes a GAG-dCAS9-VPR fusion for targeted transcriptional activation.

    Depositor

    Philippe Mangeot, Emiliano Ricci, Théophile Ohlmann

    Article

    Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

    Insert

    GAG (FMLV)-dCAS9-VPR
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV-DeAct-GS1

    Plasmid

    #89445

    Purpose

    Expresses EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells

    Depositor

    Brad Zuchero

    Article

    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

    Insert

    DeAct-GS1 (GSN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Truncation spanning cytoplasmic Gsn amino acids 5…

    Promoter

    CMV

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. GAG-CRErec

    Plasmid

    #119971

    Purpose

    Expresses GAG (FMLV) fused with CRE recombinase for the production of VLPs loaded with CRE protein

    Depositor

    Philippe Mangeot, Emiliano Ricci, Théophile Ohlmann

    Article

    Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

    Insert

    GAG-nlsCRErec
    

    Use

    Tags

    nls

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMVtrunc Affimer6-sfCherry2ΔN12

    Plasmid

    #231551

    Purpose

    Mammalian expression of Affimer6 fused to N-terminally truncated superfolder Cherry2, for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    Affimer6
    

    Use

    Tags

    sfCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    CMVtrunc

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. AICSDP-34: TNNI1-mEGFP

    Plasmid

    #114411

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TNNI1, via excisable Cas9-excisable hPGK-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

    Insert

    TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TNNI1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. AAV-hSyn-Ribo-jGCaMP8s

    Plasmid

    #167572

    Purpose

    DEPRECATED: Please see Addgene item #169247 for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Has Service

    AAV1

    Insert

    Ribo-GCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pTetON-DeAct-GS1

    Plasmid

    #89454

    Purpose

    Tet/Dox-inducible expression of EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells

    Depositor

    Brad Zuchero

    Article

    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

    Insert

    DeAct-GS1 (GSN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Truncation spanning cytoplasmic Gsn amino acids 5…

    Promoter

    TRE3G

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3.1-HA-CCND1

    Plasmid

    #172649

    Purpose

    Expresses HA-tagged cyclin D1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pcDNA3.1-HA-CDK2

    Plasmid

    #172613

    Purpose

    Expresses HA-tagged CDK2 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDK2 (CDK2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. mouse Oct4-GFP GOF18 transgenic reporter

    Plasmid

    #60527

    Purpose

    BAC recombineering generated construct, used as a reporter for Oct4 expression (containes both DE and PE elements)

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Insert

    GFPpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Oct4

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. AAV-hSyn-Ribo-jGCaMP8m

    Plasmid

    #167574

    Purpose

    DEPRECATED: Please see Addgene item #169248 for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Has Service

    AAV1

    Insert

    Ribo-GCaMP8m
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pENTR223.1_mmDdx58 (closed)

    Plasmid

    #160107

    Purpose

    Expresses murine Ddx58 in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Ddx58 (Rigi Mouse)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. AAV-hSyn-Ribo-jGCaMP8f

    Plasmid

    #167573

    Purpose

    DEPRECATED: Please see Addgene item #169249 (coming soon) for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    Ribo-GCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. FOR human OCT4 TALEN

    Plasmid

    #52412

    Purpose

    TALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939)

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Insert

    HD NG NN NN NN HD NG HD NG HD HD HD NI NG
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    CAGGS

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

49. pcDNA3.1 myc-mβTrCP WD1-7 (241-569)

    Plasmid

    #62977

    Purpose

    Expresses myc-tagged mouse βTrCP residues 241-569

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    BTRC (Btrc Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    codes for residues 241-569

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

50. pLCHKO_intergenic-intergenic_1

    Plasmid

    #155077

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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