We narrowed to 59,230 results for: cloning

1. AiO-Cas12a: All in one enAsCas12a expression and gRNA cloning backbone

   Plasmid

   #228364

   Purpose

   mU6-driven expression of enAsCas12a gRNAs with EF1a-enAsCas12a-2A-Puro

   Depositor

   Michael Boettcher

   Article

   El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral; Enascas12a nuclease and en…

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac Dual His6 MBP N10 TEV LIC cloning vector (5C)

   Plasmid

   #30123

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   His6-MBP-N10-TEV

   Expression

   Insect

   Available Since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac LIC cloning vector with BioBrick polycistronic restriction sites (11A)

   Plasmid

   #48294

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Nov. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET His6 TEV cloning vector with BioBrick polycistronic restriction sites (9B)

   Plasmid

   #48284

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Sept. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET StrepII TEV cloning vector with BioBrick polycistronic restriction sites (9R)

   Plasmid

   #48290

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   StrepII

   Expression

   Bacterial

   Available Since

   Sept. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET His6 High pI protein 1 TEV LIC cloning vector (2-HpI-1)

   Plasmid

   #55210

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, High pI protein 1, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET His6 High pI protein 85 TEV LIC cloning vector (2-HpI-85)

   Plasmid

   #55213

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, High pI protein 85, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET His6 High pI protein 68 TEV LIC cloning vector (2-HpI-68)

   Plasmid

   #55211

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, High pI protein 68, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET His6 GSSGSS NusA TEV LIC cloning vector (2NTL)

   Plasmid

   #55214

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, GSSGSS linker, NusA, and TEV cleavage site…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET His6 High pI protein 69 TEV LIC cloning vector (2-HpI-69)

    Plasmid

    #55212

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, High pI protein 69, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET FLAG TEV cloning vector with BioBrick polycistronic restriction sites (9L)

    Plasmid

    #48288

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    FLAG

    Expression

    Bacterial

    Available Since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET MYFQSNA tagged cloning vector with BioBrick polycistronic restriction sites (9U)

    Plasmid

    #48293

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    MYFQSNA

    Expression

    Bacterial

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJT050 lenti pEF-rTetR-LibCloneSite-T2A-mCherry-BSD-WPRE

    Plasmid

    #161925

    Purpose

    Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    pEF

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac His6 TEV cloning vector with BioBrick polycistronic restriction sites (11B)

    Plasmid

    #48295

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Tags

    His6

    Expression

    Insect

    Available Since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET His6 Sumo TEV cloning vector with BioBrick polycistronic restriction sites (9S)

    Plasmid

    #48291

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6 and Sumo

    Expression

    Bacterial

    Available Since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET His6 Thioredoxin TEV cloning vector with BioBrick polycistronic restriction sites (9T)

    Plasmid

    #48292

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6 and Thioredoxin

    Expression

    Bacterial

    Available Since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (9C)

    Plasmid

    #48286

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6 and MBP

    Expression

    Bacterial

    Available Since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET His6 Mocr TEV cloning vector with BioBrick polycistronic restriction sites (9O)

    Plasmid

    #48289

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6 and Mocr

    Expression

    Bacterial

    Available Since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJT216 Lenti pEF-dCas9-3XFLAG-LibCloneSite-T2A-tagBFP-P2A-Blast

    Plasmid

    #187320

    Purpose

    dCas9 expression vector with cloning site for C terminal fusions

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET C-terminal TEV His6 cloning vector with BioBrick polycistronic restriction sites (9Bc)

    Plasmid

    #48285

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Nov. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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