We narrowed to 13,587 results for: TIM

1. pAAV-CAG-fDIO-oG-WPRE-SV40pA

   Plasmid

   #74291

   Purpose

   pAAV vector to express oG (optimized Glycoprotein) in a Flp-dependent manner

   Depositor

   Edward Callaway

   Article

   Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

   Insert

   oG (optimized Glycoprotein)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   chimeric glycoprotein

   Promoter

   CAG

   Available since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNPK-NLS-Vika

   Plasmid

   #79969

   Purpose

   expresses human codon-optimized Vika-recombinase in mammalian cells

   Depositor

   Frank Buchholz

   Article

   Karimova et al Nucleic Acids Res. 2013 Jan;41(2):e37. d

   Insert

   human codon-optimized Vika sequence, NLS tagged
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCCL/IFNB1-d2eGFP-3’UTR

   Plasmid

   #180232

   Purpose

   Reporter plasmid utilizing d2eGFP as a reporter gene. Expression is designed to mimick IFNB1

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Front Cell Infect Microbiol. 2022 Jan 18

   Insert

   d2eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   1 kb upstream of IFNB1 CDS

   Available since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiCRISPRv2_mSTING_scrambled_gRNA_2

   Plasmid

   #196628

   Purpose

   Scrambled control 2 for STING knock-out in murine cells.

   Depositor

   Nicolas Manel

   Article

   Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

   Insert

   mSTING scrambled gRNA 2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. tetO.Nfia.Blast

   Plasmid

   #117270

   Purpose

   Expresses Nfia and blasticidin resistance gene under control of tetO sequence.

   Depositor

   Henrik Ahlenius

   Article

   Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

   Insert

   Nfia (Nfia Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tet-on

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-hACE2-LgBiT

   Plasmid

   #215837

   Purpose

   Expresses hACE2 receptor with LgBiT tag in mammalian cells

   Depositor

   Thomas Gallagher

   Article

   Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

   Insert

   hACE2-LgBiT (ACE2 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBBR1.cas9-NT

   Plasmid

   #197871

   Purpose

   Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)

   Depositor

   Baojun Wang

   Article

   Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72

   Inserts

   trancCRNA
   Cas9
   crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHDM-Hgpm2

   Plasmid

   #164441

   Purpose

   Encodes codon-optimized HIV gagpol for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   Codon Optimized HIV-1 GagPol
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pN251

   Plasmid

   #188471

   Purpose

   Lentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)

   Depositor

   Le Cong

   Article

   Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

   Insert

   enCas12a-HF (gift from Dr. Keith Joung)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF1a-mMLH1dn

    Plasmid

    #174825

    Purpose

    Mammalian expression of mouse MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    mouse MLH1dn (codon optimized)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of residues 758-760

    Promoter

    EF1a

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. p415 TEF roGFP2‐Tsa2ΔCR

    Plasmid

    #83238

    Purpose

    The genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

    Depositor

    Tobias Dick

    Article

    Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

    Insert

    roGFP2-Tsa2dCr
    

    Use

    Tags

    roGFP2 fusion with Tsa2dCr

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQE-60 Grx1-roGFP2-His

    Plasmid

    #64799

    Purpose

    Bacterial expression of His-tagged Grx1-roGFP2

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Tags

    His and roGFP2

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. piggyBAC-H2B FR-MQV

    Plasmid

    #194079

    Purpose

    Expresses nuclear-localized FR-MQV in mammalian cells

    Depositor

    Ralph Jimenez

    Article

    Mukherjee et al Biochemistry. 2020 Oct 6;59(39):3669-368

    Insert

    FR-MQV
    

    Use

    Tags

    Histone H2B

    Expression

    Mammalian

    Mutation

    Methionine 42 to Glutamine, Cystiene 159 to Valin…

    Promoter

    CMV7

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS-Hsp70-Cas9

    Plasmid

    #46294

    Purpose

    A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.

    Depositor

    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

    Article

    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

    Insert

    codon optimized Cas9
    

    Use

    CRISPR

    Tags

    3x Flag

    Expression

    Insect

    Mutation

    Silent change from C to A at bp 834 of insert

    Promoter

    Hsp70

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S

    Plasmid

    #62458

    Purpose

    expresses his-tagged C151S human Keap1 protein

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Keap1 (KEAP1 Human)
    

    Use

    Tags

    His-Halo-Tev

    Expression

    Mammalian

    Mutation

    Changed Cysteine 151 of Keap1 to serine

    Promoter

    CMV

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Puro_siKD

    Plasmid

    #86695

    Purpose

    Tetracycline inducible expression of shRNA targeted to AAVS1 locus

    Depositor

    Ludovic Vallier

    Article

    Bertero et al Development. 2016 Dec 1;143(23):4405-441

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 TO

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Beta1-GFP in pHcgreen

    Plasmid

    #69804

    Purpose

    Expresses Beta1-GFP integrin for normal cell transfection

    Depositor

    Martin Humphries

    Article

    Parsons et al J Cell Sci. 2008 Feb 1;121(Pt 3):265-71.

    Insert

    Beta1A integrin (ITGB1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. ErkKTR-irFP

    Plasmid

    #111510

    Purpose

    Reporter of Erk activity

    Depositor

    Jared Toettcher

    Article

    Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

    Insert

    ERK KTR (Mapk1 Mouse)
    

    Use

    Lentiviral

    Tags

    iRFP

    Expression

    Mutation

    Promoter

    SSFV

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB_EF1a-DjCas13d-msfGFP-Blast

    Plasmid

    #226008

    Purpose

    DjCas13d protein for RNA targeting in piggyBac backbone

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Insert

    DjCas13d RNA-targeting nuclease
    

    Use

    CRISPR

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSW002-Pc-mNeonGreen

    Plasmid

    #205016

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens)

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mNeonGreen is codon optimized for expression in P…

    Promoter

    Pc

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. Gal4BD-CRY2(535)

    Plasmid

    #75369

    Purpose

    Yeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535)

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    Gal4BD

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCMV-T7-AcrIIC3-NLS(SV40) (KAC209)

    Plasmid

    #134341

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIC3 with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIC3
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLPCX cyto Grx1-roGFP2

    Plasmid

    #64975

    Purpose

    Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Retroviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLNCX2-mCherry-CHMP2B

    Plasmid

    #115331

    Purpose

    expresses mCherry-CHMP2B in mammalian cells

    Depositor

    Sanford Simon

    Article

    Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

    Insert

    CHMP2B (CHMP2B Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    silent mutation in position 4205 C to T

    Promoter

    CMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. CMV500 A-CREB

    Plasmid

    #33371

    Depositor

    Charles Vinson

    Article

    Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

    Insert

    CREB (Creb1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Dominant Negative (see notes)

    Promoter

    CMV

    Available since

    Dec. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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26. mEGFP-HRas G12V

    Plasmid

    #18666

    Depositor

    Karel Svoboda

    Article

    Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

    Insert

    H-Ras (HRAS Human)
    

    Use

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Mutation

    G12V mutation in HRas

    Promoter

    Available since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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27. CRY2(535, L348F)-mCherry

    Plasmid

    #75372

    Purpose

    Expresses fusion of Arabidopsis CRY2 (residues 1-535, L348F)-mCherry

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

    Insert

    CRY2 residues 1-535 (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    L348F

    Promoter

    CMV

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CRY2PHR(W349R)-mCherry

    Plasmid

    #75370

    Purpose

    Expresses fusion of Arabidopsis CRY2 (residues 1-498, W349R fast-cycling mutant) fused to mCherry

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

    Insert

    CRY2 residues 1-498 (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    W349R

    Promoter

    CMV

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFN18A-HaloTag-Biotin

    Plasmid

    #206039

    Purpose

    HaloTag-(Ig32)2-Talin R3-IVVI-(Ig32)2-10xHisTag-AviTag

    Depositor

    Sergi Garcia-Manyes

    Article

    Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

    Insert

    Talin R3-IVVI (Tln1 Mouse)
    

    Use

    Tags

    10 x His-AviTag and Halo-Tag

    Expression

    Bacterial

    Mutation

    Threonine 809 to Isoleucine, Threonine 833 to Val…

    Promoter

    T7

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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30. pLPCX mito Grx1-roGFP2

    Plasmid

    #64977

    Purpose

    Mammalian expression of mitochondrial Grx1-roGFP2 (retroviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Retroviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    mitochondrial targeting sequence

    Promoter

    Available since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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