Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 9,280 results for: Pol

Clear keywords
Showing: 5951 - 6000 of 9280 results

  12. pTSlb-Q758C

    Plasmid
    #60729
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations Q758C
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Available Since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    60729-plasmid-map-sequence-id-99392

    Enlarge

  17. pDP1.2

    Plasmid
    #171092
    Purpose
    Expresses cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (1-110). AKA ROLECCS G2(allows integration into AAVS1 safe-harbor)
    Depositor
    Dario Palmieri
    Article
    Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1
    Insert
    AAVS1-CMV-OsTIR1-mEmerald-Geminin-Puro-AAVS1
    Use
    Synthetic Biology
    Tags
    Emerald, Geminin
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    171092-plasmid-map-sequence-id-339101

    Enlarge

  29. pTSara-R756K

    Plasmid
    #60727
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756K. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Available Since
    Oct. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    60727-plasmid-map-sequence-id-97715

    Enlarge

  34. C211

    Plasmid
    #174360
    Purpose
    For expressing siRNA-resistant human PABPC1 mutant (I110L, D111E, D117E, A121G, G139A, Y140F, T147S, R166K) in human cell lines via transfection
    Depositor
    David Bartel
    Article
    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1
    Insert
    PABPC1 (PABPC1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    I110L, D111E, D117E, A121G, G139A, Y140F, T147S, …
    Available Since
    Feb. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    174360-plasmid-map-sequence-id-343304

    Enlarge

  39. p3E DN Kif5a

    Plasmid
    #105973
    Purpose
    3' element for Gateway cloning, 5 single nucleotide mutations compared to the reference sequence, but none of them change the amino acidtruncation aa416-1033(end) 1854 bp
    Depositor
    Caren Norden
    Article
    Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10
    Insert
    Kif5a (kif5aa Zebrafish)
    Expression
    Bacterial
    Mutation
    truncation of aa 416-1033
    Available Since
    Feb. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105973-plasmid-map-sequence-id-203068

    Enlarge

Showing: 5951 - 6000 of 9280 results