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We narrowed to 6,963 results for: RAP

Showing: 5951 - 6000 of 6963 results

  2. AAV Intein-C-SpyCas9, CMV-AcrIIA4-2xmiR-122 target sites

    Plasmid
    #120295
    Purpose
    AAV Vector for expression of C-terminal SpyCas9 fragement with split-intein and a CMV-driven AcrIIA4 with two miR-122 binding sites
    Depositor
    Dominik Niopek
    Article
    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545
    Insert
    C-terminal fragment of SpyCas9
    Use
    AAV and CRISPR
    Tags
    split-intein
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    120295-plasmid-map-sequence-id-232737

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  3. pLKO.1-TRC.mKO2_shHRASLS.2

    Plasmid
    #110324
    Purpose
    TRCN0000002620 (Target GCGATAAGTACCGTTGAGTTT), silence human HRASLS gene and express monomeric Kusabira-Orange2
    Depositor
    Sandra Martha Gomes Dias
    Article
    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1
    Insert
    HRASLS (PLAAT1 Human, Homo sapiens)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    RNA polymerase III promoter for human U6 snRNA fo…
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110324-plasmid-map-sequence-id-213397

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  4. pLKO.1-TRC.mKO2_shHRASLS.1

    Plasmid
    #110323
    Purpose
    TRCN0000378630 (Target GATAAGTACCGTTGAGTTTG), silence human HRASLS gene and express monomeric Kusabira-Orange2
    Depositor
    Sandra Martha Gomes Dias
    Article
    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1
    Insert
    HRASLS (PLAAT1 Human, Homo sapiens)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    RNA polymerase III promoter for human U6 snRNA fo…
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110323-plasmid-map-sequence-id-213396

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  5. Adeno BN

    Plasmid
    #101823
    Purpose
    Adenovirus for the expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1
    Depositor
    Andrea Ventura
    Article
    Cook et al Nat Commun. 2017 Jul 11;8:15987. doi: 10
    Insert
    U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)_CBh_FLAG-Cas9 (Bcan, Ntrk1 Mouse)
    Use
    Adenoviral
    Tags
    FLAG-Cas9
    Expression
    Mutation
    Promoter
    Available since
    Nov. 8, 2017
    Availability
    Academic Institutions and Nonprofits only

  6. pETM6-1D4-mCherry

    Plasmid
    #73423
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1D4.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 1D4 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P1D4 (orthogonal T7-lac variant)
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73423-plasmid-map-sequence-id-135323

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  7. pETM6-4A6-mCherry

    Plasmid
    #73424
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4A6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 4A6 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P4A6 (orthogonal T7-lac variant)
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73424-plasmid-map-sequence-id-135324

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  8. pETM6-1E4-mCherry

    Plasmid
    #73426
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1E4.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 1E4 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P1E4 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73426-plasmid-map-sequence-id-135326

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  9. pETM6-1B6-mCherry

    Plasmid
    #73420
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1B6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 1B6 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P1B6 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73420-plasmid-map-sequence-id-135320

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  10. pETM6-3H5-mCherry

    Plasmid
    #73419
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3H5.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 3H5 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P3H5 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73419-plasmid-map-sequence-id-135319

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  23. pLV-TetO-hNGN2-eGFP-Puro

    Plasmid
    #79823
    Purpose
    Expresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.
    Depositor
    Kristen Brennand
    Article
    Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15
    Insert
    hNGN2-P2A-eGFP-T2A-PuroR (NEUROG2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    TetO
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79823-plasmid-map-sequence-id-240610

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  30. PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA

    Plasmid
    #166693
    Purpose
    Express dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.
    Depositor
    Elvir Becirovic
    Article
    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi
    Inserts
    dCas9-VPR
    3x Cnga1 promoter-targeting sgRNAs
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE and U6
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    166693-plasmid-map-sequence-id-331043

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  35. pMXs-IP-mCherry-TOSI

    Plasmid
    #172496
    Purpose
    mCherry-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.
    Depositor
    David Scadden
    Article
    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:
    Insert
    PDCD4 (Pdcd4 Mouse)
    Use
    Retroviral
    Tags
    Mcherry
    Expression
    Mammalian
    Mutation
    Promoter
    MLV LTR
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    172496-plasmid-map-sequence-id-338550

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  36. pMXs-IP-mVenus-TOSI

    Plasmid
    #172491
    Purpose
    mVenus-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.
    Depositor
    David Scadden
    Article
    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:
    Insert
    Pdcd4 (Pdcd4 Mouse)
    Use
    Retroviral
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    MLV LTR
    Available since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    172491-plasmid-map-sequence-id-346537

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  39. pCDH-SFFV-GLuc-CreERT2-mCherry

    Plasmid
    #178185
    Purpose
    the plasmid promotes the constitutive expression of CreERT2 to enable intracellular recombination, Gaussia Luc can be used for in vivo imaging and mCherry is used as a marker for ex vivo analyses.
    Depositor
    Irmela Jeremias
    Article
    Carlet et al Nat Commun. 2021 Sep 27;12(1):5655. doi:
    Inserts
    GLuc
    CreERT2
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    178185-plasmid-map-sequence-id-351314

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Showing: 5951 - 6000 of 6963 results