We narrowed to 10,180 results for: yeast
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Plasmid#204287PurposeFor C-terminal tagging using yeast optimized mRuby3DepositorInsertpFA6-yomRuby3-SpHIS5
TagsyomRuby3ExpressionYeastPromoternoneAvailable SinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-puro-Venus-FLAG-CD59
Plasmid#50377PurposeExpress Venus and FLAG-tagged human CD59, a GPI-AP in mammalian cellsDepositorInsertVenus
ExpressionMammalianPromoterSR alphaAvailable SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDuRCC-N
Plasmid#81194PurposeExpression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin ResistanceDepositorInsertsCas9
empty gRNA cassette
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease view depositor comments below and WT - cod…PromoterROX3 and SNP52pAvailable SinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
BAS286 C terminal SF-GFP hygBR
Plasmid#74081PurposeS.pombe recoded superfolder GFP in universal C-terminal tagging plasmid, hygB resistancDepositorInsertsuper-folder GFP
Available SinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJZC588
Plasmid#62315PurposesgRNA with 2x MS2 (wt+f6) for yeast cellsDepositorInsertsgRNA + 2x MS2 (wt+f6) binding module
ExpressionYeastPromoterSNR52Available SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AU1-mTOR-L1460P
Plasmid#19995DepositorInsertmammalian target of rapamycin-L1460P (Mtor Rat)
TagsAU1ExpressionMammalianMutationChanged Leucine 1460 to ProlineAvailable SinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
BB1_12_pGAP
Plasmid#98498PurposeBB1 with insert: promoter PGAPDepositorInsertGAP promoter
ExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-trAtCCD7-OsD27(pYL726 )
Plasmid#178285PurposeCo-Expresses OsD27 and trAtCCD7 in E. coli. pCDFDuet-1 carrying D27 from Oryza sativa and N-terminus 31 amino-acid truncated CCD7 from ArabidopsisDepositorInsertsOsD27
trAtCCD7
ExpressionBacterialPromoterT7Available SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-HA-hApg12
Plasmid#22950DepositorAvailable SinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJZC603
Plasmid#62317PurposesgRNA with 2x PP7 for yeast cellsDepositorInsertsgRNA + 2x PP7 RNA binding module
ExpressionYeastPromoterSNR52Available SinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIMVAd40R
Plasmid#98309PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of GAL promoters; integrated into ERG9 locus;DepositorInsertERG9C-terminal-TURA3- PGAL7>MVD1>TPRM9-PGAL2>-ERG12>TNATTIDP1ExpressionYeastAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mTIP49a
Plasmid#15357DepositorInsertTATA binding protein interacting protein 49 kDa (Ruvbl1 Mouse)
TagsFLAGExpressionMammalianAvailable SinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEasyG1_hph
Plasmid#184912PurposeTemplate to generate via PCR a single gRNA for expression in S. cerevisiaeDepositorInsertgRNA scaffold
UseCRISPRExpressionBacterial and YeastAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGAN147
Plasmid#129202Purposeyeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backboneDepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastPromoterSUP35Available SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJZC545
Plasmid#62313PurposesgRNA with 1x MS2 for yeast cellsDepositorInsertsgRNA + 1x MS2 binding module
ExpressionYeastPromoterSNR52Available SinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWL89A mmPing20
Plasmid#145788PurposemPing Yeast Transposition AssayDepositorInsertmmPing20
ExpressionYeastMutationT162C, T258A, T287C, T300A, T304A, T310A, G372AAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
BAS285 C terminal mKO2 hygBR
Plasmid#74080PurposeS.pombe recoded mKO2 in universal C-terminal tagging plasmid, hygB resistance.DepositorInsertmKO2
Available SinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSCL.028
Plasmid#184965PurposeTest effect of extending -Eco1 a1/a2 in yeastDepositorInsertEco1: RT and ncRNA(extended), a1/a2 length: 27
ExpressionYeastMutationhuman codon optimized RT, a1/a2 length extended t…PromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pV1539
Plasmid#111434PurposeGenedrive entry vector for Candida albicansDepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
prp8-G736E
Plasmid#108450Purposeprp8-G736E in pRS313 backbone for expression in yeastDepositorAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCT
Plasmid#60620PurposePlasmid encoding iCas9 and tracrRNA on pRS415 backboneDepositorInsertsiCas9
tracrRNA
UseCRISPRTagsFLAG and SV40 NLSExpressionYeastMutationchanged Aspartate 147 to Tyrosine, Proline 411 to…PromoterRPR1p and TEF1pAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
BAS288 C terminal mKO2 natR
Plasmid#74083PurposeS.pombe recoded mKO2 in universal C-terminal tagging plasmid, nourseothricin resistance.DepositorInsertmKO2
Available SinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJZC583
Plasmid#62314PurposesgRNA with 2x MS2 for yeast cellsDepositorInsertsgRNA + 2x MS2 binding module
ExpressionYeastPromoterSNR52Available SinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pV1464
Plasmid#111440PurposeN. castellii Solo CRISPR vector, marked with URA3 and NatRDepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Amida
Plasmid#15362DepositorAvailable SinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJT43_GalL_BE-P(AP)3
Plasmid#145036PurposeExpresses BE-P(AP)3 in yeast cellsDepositorInsertBE-(AP)3
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLLX13
Plasmid#79825PurposeYeast and Bacterial shuttle vector, includes origin of transfer for conjugation, used for making a DNA capture vectorDepositorTypeEmpty backboneUseYeast and bacteria shuttle vector, contains origi…Available SinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS415-Cas9-VPR
Plasmid#163971PurposeTrifunctional Cas9-VPR fusion protein plasmid for simultaneous transcriptional activation, transcriptional repression, and genome editing in yeastDepositorInsertCas9-VPR
UseSynthetic BiologyExpressionYeastAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTPI1-EMC1-GFP-APEX2-URA3
Plasmid#184776PurposeExpresses EMC1-GFP-APEX2 chimera for labeling yeast endoplasmic reticulum (ER). Uses auxotrophic marker URA3(Kluyveromyces lactis).DepositorInsertAPEX2
ExpressionYeastAvailable SinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
BAS287 C terminal E2C hygBR
Plasmid#74082PurposeS.pombe recoded E2Crimson in universal C-terminal tagging plasmid, hygB resistance.DepositorInsertE2Crimson
Available SinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-Myc-hApg12
Plasmid#22951DepositorAvailable SinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRS405 PEX14-VAC8-RFP
Plasmid#166844PurposeExpresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORFDepositorTagsPEX14 transmembrane domain and RFPExpressionYeastMutationPEX14 transmembrane domainPromoterVAC8 promoterAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB1975 (pUC19-PTEF1-PPGK1)
Plasmid#73296PurposeExample plasmid containing a bidirectional promoterDepositorInsertpTEF1-pPGK1
UseSynthetic BiologyExpressionBacterial and YeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJZC593
Plasmid#62319PurposesgRNA with MS2-PP7 for yeast cellsDepositorInsertsgRNA + MS2-PP7 RNA binding module
ExpressionYeastPromoterSNR52Available SinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJZC572
Plasmid#62318PurposesgRNA with 1x com for yeast cellsDepositorInsertsgRNA + 1x com RNA binding module
ExpressionYeastPromoterSNR52Available SinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Desmin/pAS2-1
Plasmid#97407PurposeDesmin (aa 30 – 103) in DNA-binding domain (BD) vector, used for yeast-two hybridDepositorInsertDesmin (DES Human)
ExpressionYeastAvailable SinceAug. 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET32b-FLJ23445
Plasmid#15434DepositorInsertFLJ23445
TagsHis and TrxExpressionBacterialMutationN/AAvailable SinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T2-Snf1-cat
Plasmid#52683Purposeexpression of the Snf1 kinase domain in bacteriaDepositorInsertSNF1 (SNF1 Budding Yeast)
TagsGSTExpressionBacterialMutationencodes kinase domain only, aa 41-315PromotertacAvailable SinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
BB1_34_RPS25Att
Plasmid#98526PurposeBB1 with insert: RPS25AttDepositorInsertRPS25Att
ExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS406 ATG1-ATG17-3'UTR
Plasmid#166848PurposeExpresses Atg1 with ATG17 3'UTR in yeasts.DepositorAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS316-RGR-GFP-mm
Plasmid#51059PurposeSame as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end, and for the HDV ribozyme region which has a 15 bp deletion at its 3' end.DepositorInsertRGR-GFP
UseCRISPRExpressionBacterial and YeastPromoterYeast ADH1 promoterAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
BAS289 C terminal E2C G418R
Plasmid#74084PurposeS.pombe recoded E2Crimson in universal C-terminal tagging plasmid, G418 resistanceDepositorInsertE2Crimson
Available SinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJKW1847
Plasmid#174004PurposeExpresses PpCYP94D108 and AtATR1 for reconstitution of diosgenin biosynthesis in yeastDepositorInsertsExpressionYeastAvailable SinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
BB1_23_eGFP
Plasmid#98518PurposeBB1 with insert: CDS eGFPDepositorInserteGFP
ExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH737-CEN-maxHIS3
Plasmid#38229DepositorInsertHIS3 (HIS3 Budding Yeast, Synthetic)
TagsHAExpressionYeastMutationEvery codon of the coding sequence has been repla…PromoterTDH3 (=GPD)Available SinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
BB1_12_pLAT1
Plasmid#98509PurposeBB1 with insert: promoter PLAT1DepositorInsertLAT1 promoter
ExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB1_12_pPFK300
Plasmid#98510PurposeBB1 with insert: promoter PPpPfkDepositorInsertPpPfk promoter
ExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB1_12_pDAS2
Plasmid#98513PurposeBB1 with insert: promoter PDAS2DepositorInsertDAS2 promoter
ExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
BAS291 C terminal mKO2 kanR
Plasmid#74086PurposeS.pombe recoded mKO2 in universal C-terminal tagging plasmid, G418 resistance.DepositorInsertmKO2
Available SinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
BB1_12_pRPL2A
Plasmid#98508PurposeBB1 with insert: promoter PRPL2ADepositorInsertRPL2A promoter
ExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only