Addgene

Showing: 2421 - 2440 of 2899 results

Results per page:

Sort results by:

pDONRp5-p2 GFP-NShroom3-iLID

Plasmid

#170976

Purpose

N-terminal component of OptoShroom3 optogenetic tool. Binds to apical junctions. pDONRp5-p2 plasmid.

Depositor

Miki Ebisuya

Article

Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

Insert

NShroom3 (Shroom3 Mouse)

Use

Gateway cloning

Tags

eGFP and iLID

Expression

Mutation

From isoform 2, deleted amino acids 1389 - 1805,…

Promoter

Available since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hsyn-ASAP5-WPRE

Plasmid

#225709

Purpose

Cre-dependent pan-membrane expression of the genetically encoded voltage indicator ASAP5, can be used for dendritic voltage imaging

Depositor

Michael Lin

Article

Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

Insert

ASAP5

Use

AAV

Tags

Expression

Mutation

Promoter

human synapsin

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-FUSN-mCh-Cry2olig

Plasmid

#101224

Purpose

FUS(1-214) fused to mCh-Cry2olig

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

FUS(aa1-214) (FUS Human)

Use

Lentiviral

Tags

mCherry-Cry2olig

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CRY2-mCherry-Miro1TM

Plasmid

#102247

Purpose

Expresses fusion of CRY2PHR with mCherry and transmembrane domain of Miro1

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

CRY2PHR-mCherry-Miro1TM (RHOT1, CRY2 Mustard Weed, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-dDIO hChR2(H134R)-EYFP

Plasmid

#55640

Purpose

Dre-Dependent ChR2-EYFP

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

hChR2(H134R)-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 8, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pNMSB84

Plasmid

#199309

Purpose

MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

Depositor

Michael Krieg

Article

Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

Insert

flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR

Use

Tags

Expression

Worm

Mutation

mTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…

Promoter

flp-18

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hsyn-ASAP5-Kv2.1-WPRE

Plasmid

#225707

Purpose

Pan-neuronal soma-targeted expression of the genetically encoded voltage indicator ASAP5

Depositor

Michael Lin

Article

Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

Insert

ASAP5-Kv2.1

Use

AAV

Tags

Expression

Mutation

Promoter

human synapsin

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-vCreDIO hChR2(H134R)-EYFP

Plasmid

#55643

Purpose

vCre-Dependent ChR2-EYFP

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

hChR2(H134R)-EYFP

Use

AAV; Vcre-dependent chr2-eyfp

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 8, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-fDIO hChR2(H134R)-EYFP

Plasmid

#55639

Purpose

Flp-Dependent ChR2-EYFP

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde

Insert

hChR2(H134R)-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-syn-FLEX-splitTVA-EGFP-tTA

Plasmid

#100798

Purpose

helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G

Depositor

Ian Wickersham

Article

Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

Has Service

AAV1

Insert

TVA, EGFP, tet transactivator

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

synapsin

Available since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-IP-EGFP-LOV2-Cyb5a

Plasmid

#233348

Purpose

Stable expression of EGFP-LOV2(N538E)-Cyb5a in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.

Depositor

Noboru Mizushima

Article

Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

Inserts

LOV2 domain of phototropin1
Cyb5a (Cyb5a Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

N-terminus is deleted (Cyb5a 95-134) and N538E

Promoter

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
opto-E-cad GFP

Plasmid

#203327

Purpose

Expresses E-cadherin with a LOV2 insert after T133 for blue light inhibition in mammalian cells

Depositor

Seraphine Wegner

Article

Nzigou Mombo et al Nat Commun. 2023 Oct 9;14(1):6292. doi:

Insert

Photoswitchable - E-cadherin with GFP tag (CDH1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Addition of Aslov2 domain at T133 of human E-cadh…

Promoter

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPBbsr2-rtTA2-TRE-SspB-mScarlet-I-MYPT169

Plasmid

#178528

Purpose

Expresses rtTA and SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

rtTA, SspB-mScarlet-I-MYPT (1-169aa)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG, Tet responsive element

Available since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pNMSB104

Plasmid

#199314

Purpose

MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

Depositor

Michael Krieg

Article

Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

Insert

flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR

Use

Tags

Expression

Worm

Mutation

mTagBFP2 from pJJR81, C. elegans codon optimized …

Promoter

flp-18

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Syn-ChrimsonR-GFP

Plasmid

#58806

Purpose

AAV expression of ChrimsonR-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

K176R

Promoter

Syn

Available since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
eMscL WT

Plasmid

#107454

Purpose

eMscL WT is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif

Depositor

Francesco Difato

Article

Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393

Insert

bacterial mechanosensitive ion channel of large conductance (mscL Escherichia Coli)

Use

Adeno-associated viral

Tags

Kir2.1 ER export signal (FCYENEV) and tdTomato fl…

Expression

Mammalian

Mutation

Promoter

human synapsin 1

Available since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-nEF-ChRmine-mScarlet

Plasmid

#137158

Purpose

Viral expression of ChRmine-p2a-mScarlet

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

ChRmine-p2a-mScarlet

Use

AAV

Tags

Expression

Mutation

n/a

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-pCMV-TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA

Plasmid

#171617

Purpose

AAV Soma-targeted Cal-Light with KA2

Depositor

Hyungbae Kwon

Article

Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

Insert

TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA

Use

AAV and Synthetic Biology

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn Con/Fon EYFP

Plasmid

#55650

Purpose

Cre-on/Flp-on EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde and AAV8

Insert

eYFP with introns

Use

AAV; Cre on/flp on eyfp

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-VTKS1-hChR2-tBFP

Plasmid

#178706

Purpose

AAV vector for transgene expression of hChR2-tBFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

hChR2-tBFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge