We narrowed to 9,260 results for: Coli

1. pMBA333

   Plasmid

   #214745

   Purpose

   Medium copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

   Depositor

   Tae Seok Moon

   Article

   Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

   Insert

   BBa_J23119-RiboJ-RBS(21992)-gfpmut3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. P823_eSpCas9(1.1)-RecA

   Plasmid

   #87264

   Purpose

   Human codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiency

   Depositor

   Yonglun Luo

   Article

   Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

   Insert

   RecA
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMVA

   Plasmid

   #121149

   Purpose

   This plamid was used for improving the production of DMAPP (IPP) in E.coli

   Depositor

   Minjuan Xu

   Article

   He et al ACS Synth Biol. 2018 Sep 21;7(9):2094-21

   Insert

   MVA Pathway (ERG12, ERG8, MVD1, IDI, MvaE, MvaS )
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast

   Plasmid

   #213117

   Purpose

   Expression of a faster-running MinE mutant in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   MinE(L4E)-EGFP (minE Escherichia coli)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   L4E

   Promoter

   EF1a

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLV-EF1a-FRB-mCherry-MinD-IRES-Puro

   Plasmid

   #213116

   Purpose

   Expression of FRB-mCherry-MinD in mammalian cells for payload delivery

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   FRB-mCherry-MinD (minD Escherichia coli)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFW5000

   Plasmid

   #224214

   Purpose

   E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin), tetQ

   Depositor

   Ophelia Venturelli

   Article

   Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET303-colE1TR

   Plasmid

   #155181

   Purpose

   Expresses colicin E1 residues 1-364

   Depositor

   Joanna Slusky

   Article

   Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

   Insert

   colicin E1 residues 1-364
   

   Use

   Tags

   C-terminal 6x Histidine tag

   Expression

   Bacterial

   Mutation

   truncation of colicin E1 including 1-364

   Promoter

   T7

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJB042 (FtsZ-mEos2)

   Plasmid

   #49764

   Purpose

   Inducible expression of FtsZ-mEos2 in bacteria

   Depositor

   Jie Xiao

   Article

   Buss et al Mol Microbiol. 2013 Sep;89(6):1099-120.

   Insert

   FtsZ-mEos2
   

   Use

   Tags

   mEos2

   Expression

   Bacterial

   Mutation

   Promoter

   T5-lac

   Available since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-subtilase A

   Plasmid

   #35948

   Depositor

   Zhaoyang Feng

   Article

   Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

   Insert

   Subtilase A (subA E. Coli)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pPBCAG-rtTAM2-IH

    Plasmid

    #140827

    Purpose

    Expresses rtTAM2-IRES-HygR in mammalian cells

    Depositor

    Hideyuki Okano

    Article

    Kisa et al Stem Cell Reports. 2017 Dec 12;9(6):1825

    Inserts

    rtTAM2
    HygR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSCL.139

    Plasmid

    #184994

    Purpose

    Express Cas9-P2A-Eco1RT in human cells

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    SpCas9-P2A-Eco1RT
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    Human codon optimized RT

    Promoter

    TetOn-3g

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. aav-SYN-tdNfsB(F124W)-mCherry (eNTR)

    Plasmid

    #113760

    Purpose

    AAV-mediated expression of bacterial tdNfsB-mCherry (eNTR) under the SYN promoter

    Depositor

    Luke Lavis

    Article

    Gruber et al ACS Chem Biol. 2018 Aug 29. doi: 10.1021

    Insert

    tdNfsB(F124W)-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    F124W

    Promoter

    Synapsin

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBP-J23100

    Plasmid

    #72963

    Purpose

    Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    J23100 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPLtetO-ClpX

    Plasmid

    #89657

    Purpose

    Plasmid for titrating synthesis of ClpX with doxycycline

    Depositor

    Uwe Sauer

    Article

    Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

    Insert

    clpX
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLicC-MBP-APETx2

    Plasmid

    #72668

    Purpose

    Periplasmic expression of APETx2 in E.coli

    Depositor

    Glenn King

    Article

    Anangi et al Mar Drugs. 2012 Jul;10(7):1605-18. doi:

    Insert

    APETx2
    

    Use

    Tags

    His tag, MBP, MalE signal sequence for periplasmi…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAC-4CL1

    Plasmid

    #35947

    Depositor

    Claudia Schmidt-dannert

    Article

    Watts et al BMC Biotechnol. 2006 Mar 21;6:22.

    Insert

    4-coumaroyl CoA ligase 1 (4CL1 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLac-mod

    Available since

    Sept. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBADmod1-linker2-gamS

    Plasmid

    #45833

    Depositor

    Richard Murray, Vincent Noireaux

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    gamS
    

    Use

    Synthetic Biology

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNAS1B split mCherry N-link/link-C

    Plasmid

    #61965

    Purpose

    Negative control duet plasmid for split mCherry assembly assay (i.e., neither half of mCherry is fused to another peptide or protein)

    Depositor

    Lynne Regan

    Article

    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

    Inserts

    N-mCherry-link
    link-C-mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD and PLtetO

    Available since

    Feb. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PET-3a-chpBK

    Plasmid

    #155201

    Purpose

    To express recombinant ChpBK protein

    Depositor

    Guanzheng Luo

    Article

    Zhang et al Sci Adv. 2019 Jul 3;5(7):eaax0250. doi:

    Inserts

    chpS-chpBK-6×His-Tag
    GyrA-CBD-10xHis-Tag
    

    Use

    Tags

    10xHis and 6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pY2F

    Plasmid

    #53285

    Purpose

    Contains an Arabidopsis thaliana lcyE cDNA and produces an active lycopene epsilon-cyclase enzyme in E. coli. Use with pAC-BETA-At or pAC-BETAipi-At to produce alpha-carotene in E. coli.

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    lcyE (LUT2 Mustard Weed)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSNK

    Plasmid

    #135193

    Purpose

    Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains kamamycin resistance cassette

    Depositor

    Hongqing Fang

    Article

    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

    Insert

    I-SceI lamba Red
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    araBAD

    Available since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBEL1203

    Plasmid

    #196024

    Purpose

    Expression/purification of 6xHis-TEV-MlaC

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCNA

    Plasmid

    #135191

    Purpose

    Arabinose inducible expression of lamba-Red recombinase proteins and I-CreI.

    Depositor

    Hongqing Fang

    Article

    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

    Insert

    I-CreI lamba Red
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    araBAD

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTS2365-Tier1-PhCMV-TetR

    Plasmid

    #169566

    Purpose

    Tier-1 vector encoding PhCMV-driven tetracycline dependent regulator protein TetR (PhCMV-TetR-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven tetracycline repressor protein class B from transposon Tn10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBEL1092

    Plasmid

    #195635

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDEINO4

    Plasmid

    #179488

    Purpose

    Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

    Depositor

    Bogumil Karas

    Article

    Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

    Insert

    aadA1
    

    Use

    Synthetic Biology; Bacterial and yeast cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA5_NLS-Halo-eGFP-BioID

    Plasmid

    #218448

    Purpose

    expresses the NLS-Halo-eGFP-BioID fusion protein via stable Flp-In integration

    Depositor

    Thorsten Stafforst

    Article

    Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

    Insert

    NLS-Halo-eGFP-BirA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDR111_Bgal NLS

    Plasmid

    #188396

    Purpose

    Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.

    Depositor

    Christopher Contag

    Article

    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

    Insert

    lacZ
    

    Use

    Tags

    PhoD secretion peptide and SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Phyper spank

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRSFDuet-1_DARPinOFF7

    Plasmid

    #182396

    Purpose

    Bacterial expression of DARPinOFF7

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    DARPinOFF7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRXG

    Plasmid

    #113643

    Purpose

    This construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    mRFP1-linker-sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge