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PCRII apoA-IVb.2

Plasmid

#121904

Purpose

Plasmid for making zebrafish apoA-IVb.2 in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

Apoa4b.2 (apoa4b.2 Zebrafish)

Use

Unspecified

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pFSW Doc2beta 6X mutant

Plasmid

#128816

Purpose

To make virus expressing Doc2beta 6X mutant

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Use

Lentiviral

Tags

IRES-EGFP

Mutation

D163A, D218A, D220N, D303A, D357A, D359A

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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dCas9-KRAB-MeCP2

Plasmid

#110821

Purpose

Improved dCas9 repressor-dCas9-KRAB-MeCP2

Depositor

Alejandro Chavez, George Church

Article

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB-MeCP2

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CMV

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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dCas9-KRAB

Plasmid

#110820

Purpose

Conventional dCas9 repressor-dCas9-KRAB

Depositor

Alejandro Chavez, George Church

Article

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CMV

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/GFPZNF598-9P-9A

Plasmid

#141192

Purpose

Expresses GFP-ZNF598 mutated in polyproline streches in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

ZNF598 (ZNF598 Human)

Tags

GFP

Expression

Mammalian

Mutation

all 3 proline repeats are mutated to Alanine (9xP…

Promoter

CMV

Available Since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pB-CAGGS-dCas9-KRAB

Plasmid

#110822

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, George Church

Article

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CAGGS

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/GFP-TTP

Plasmid

#141190

Purpose

Expresses GFP-TTP in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

TTP (ZFP36 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ATG9 (692-839)

Plasmid

#188092

Purpose

To make monomeric ATG9 C-terminal tail for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (692-839)

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2 LRP6-eGFP

Plasmid

#180143

Purpose

to make movies of LRP6 vesicles

Depositor

Edward De Robertis

Article

Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

Insert

Human LRP6 (LRP6 Human)

Expression

Mammalian

Promoter

SP6

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-ATG13 (2-197)

Plasmid

#188075

Purpose

To make ATG101/ATG13 HORMA complex for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Tags

strep-strep-Flag tag

Expression

Mammalian

Mutation

ATG13 (2-197)

Promoter

CMV

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pegRNA-HEK3_del1-5-NGG

Plasmid

#185478

Purpose

pegRNA plasmid in order to make a 5 basepair deletion at the HEK3 locus, uses an NGG-PAM site.

Depositor

Sabine Fuchs

Article

Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

Insert

HEK3_del1-5-NGG pegRNA

Expression

Mammalian

Promoter

U6

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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ACTB-PQR-RFPnols-PQR-loxP-Zeo-loxP

Plasmid

#121448

Purpose

To make a stable human recombinant cell line; see right thumbnail map image for PQR annotations

Depositor

Brian Chen

Article

Lo et al Biotechniques. 2017 Apr 1;62(4):165-174.

Insert

RFPnols-PQR-ZeocinR (ACTB Human)

Use

CRISPR

Expression

Mammalian

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pB-CAGGS-dCas9

Plasmid

#110823

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, George Church

Article

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CAGGS

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAd-Track Flag HA PGC-1 alpha

Plasmid

#14426

Depositor

Pere Puigserver

Article

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Use

Adenoviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Last four amino acids of PGC-1a removed to make t…

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pD649-HAsp-SEMA3F-COMP5AP-AviTag-9xHis

Plasmid

#157541

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SEMA3F (SEMA3F Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Bcl-2-s2193

Plasmid

#166750

Purpose

Express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-2 in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

Bcl-2 (BCL2 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

Human ferritin

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pX330-mitf

Plasmid

#69801

Purpose

Used to make mitf knockout porcine cell line

Depositor

Jianguo Zhao

Article

Wang et al Sci Rep. 2015 Aug 21;5:13348. doi: 10.10

Inserts

mitf-sgRNA
SpCas9

Expression

Mammalian

Promoter

Chicken Beta-Actin and U6

Available Since

Dec. 29, 2015

Availability

Academic Institutions and Nonprofits only

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HOXB7 (human) FLAG pSP65

Plasmid

#8537

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB7 (HOXB7 Human)

Use

In vitro transcription/translation

Tags

FLAG

Expression

Bacterial

Mutation

starts at AA#1 preceeded by FLAG and Ser-Arg-Ile…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
CaMKIV-DCTK75E

Plasmid

#126423

Purpose

A kinase-dead mutant of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)

Depositor

Douglas Black

Article

Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi

Insert

calcium/calmodulin-dependent protein kinase IV kinase-dead mutant (CAMK4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed lysine 75 to glutamic acid

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL680 CHYRON3 integration construct

Plasmid

#126448

Purpose

To integrate the CHYRON3 locus at AAVS1 in human cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

pU6/3xLacO-CHYRON3 hgRNA
pCMV-puro

Expression

Mammalian

Mutation

The SpCas9 sgRNA constant region is mutated to ma…

Promoter

CMV and human U6/3xLacO

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAd-Track HA PGC-1 alpha

Plasmid

#14427

Depositor

Pere Puigserver

Article

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Use

Adenoviral

Tags

HA

Expression

Mammalian

Mutation

Last four amino acids of PGC-1a removed to make t…

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pCAGGS-RaichuEV-Rac1(T17N)

Plasmid

#164902

Purpose

Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domain

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

RaichuEV-Rac1 (RAC1 Human)

Expression

Mammalian

Mutation

T17N mutation in the Rac1 domain which make the s…

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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PCRII apoEb

Plasmid

#121901

Purpose

Plasmid for making zebrafish apoEb in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

Apoeb (apoeb Zebrafish)

Use

Unspecified

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pKIF1C-BioID2-HA

Plasmid

#130980

Purpose

Expression of HA-tagged KIF1C-BioID2

Depositor

Anne Straube

Article

Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

Insert

KIF1C-BioID2-HA (KIF1C Human)

Tags

BioID2-HA

Expression

Mammalian

Mutation

5 silent mutations that make this construct RNAi …

Promoter

CMV

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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HOXB3 (human) FLAG pSP65

Plasmid

#8523

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXB3 (HOXB3 Human)

Use

In vitro transcription/translation

Tags

FLAG

Expression

Bacterial

Mutation

Contains 5 additional amino acids before ATG from…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pCAG-MBP-Foldon-ATG9 (830-839)

Plasmid

#188091

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (830-839)

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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HOXB5 (human) FLAG-MSCV

Plasmid

#8532

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB5 (HOXB5 Human)

Use

Retroviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

contains improved ribosomal binding site and FLAG…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pCAG-MBP-Foldon-ATG9 (692-839)

Plasmid

#188087

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (692-839)

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pTBL681 CHYRON4 integration construct

Plasmid

#126449

Purpose

To integrate the CHYRON4 locus at AAVS1 in human cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

pU6/3xLacO-CHYRON4 hgRNA
pCMV-puro

Expression

Mammalian

Mutation

The SpCas9 sgRNA constant region is mutated to ma…

Promoter

CMV and human U6/3xLacO

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKIF1C-2xFLAG

Plasmid

#130979

Purpose

Expression of KIF1C-2xFLAG in mammalian cells.

Depositor

Anne Straube

Article

Theisen et al Dev Cell. 2012 Dec 11;23(6):1153-66. doi

Insert

KIF1C-2xFLAG (KIF1C Human)

Tags

2xFLAG

Expression

Mammalian

Mutation

4 silent mutations that make this construct resis…

Promoter

CMV

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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