We narrowed to 42,575 results for: cha;

1. S3_003_026_096

   Plasmid

   #134406

   Purpose

   pYTK096-pREV1_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

   Depositor

   Mattheos Koffas

   Article

   Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

   Inserts

   mCherry
   NLS_FdeR-N
   

   Use

   Tags

   none

   Expression

   Yeast

   Mutation

   none

   Promoter

   pGPM1 and pREV1

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. S3_005_026_MV1

   Plasmid

   #134417

   Purpose

   pYTKMV1-pRPL18B_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1

   Depositor

   Mattheos Koffas

   Article

   Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

   Inserts

   mCherry
   FdeR-N_2xNLS
   

   Use

   Tags

   none

   Expression

   Yeast

   Mutation

   none

   Promoter

   pGMP1 and pRPL18B

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. S3_003_026_MV1

   Plasmid

   #134415

   Purpose

   pYTKMV1-pREV1_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

   Depositor

   Mattheos Koffas

   Article

   Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

   Inserts

   mCherry
   NLS_FdeR-N
   

   Use

   Tags

   none

   Expression

   Yeast

   Mutation

   none

   Promoter

   pGMP1 and pREV1

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. S3_001_026_MV1

   Plasmid

   #134413

   Purpose

   pYTKMV1-pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

   Depositor

   Mattheos Koffas

   Article

   Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

   Inserts

   mCherry
   NLS_FdeR-N
   

   Use

   Tags

   none

   Expression

   Yeast

   Mutation

   none

   Promoter

   pGPM1 and pTDH3

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. S3_005_026_096

   Plasmid

   #134408

   Purpose

   pYTK096-pRPL18B_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1

   Depositor

   Mattheos Koffas

   Article

   Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

   Inserts

   mCherry
   FdeR-N_2xNLS
   

   Use

   Tags

   none

   Expression

   Yeast

   Mutation

   none

   Promoter

   pGPM1 and pRPL18B

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMM0652

   Plasmid

   #129013

   Purpose

   Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effects

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-KanR-ColE1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRS-ATG1(mut)(406)

   Plasmid

   #122069

   Purpose

   Expressing a mutant ATG1

   Depositor

   Daniel Klionsky

   Article

   Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

   Insert

   ATG1 (ATG1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Codon changes without changing the amino acid seq…

   Promoter

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRPL28 CaURA3 (pNTI592)

   Plasmid

   #115431

   Purpose

   URA3-marked RPS28

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   URA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   KpnI site in intron

   Promoter

   Ashbya gossypii and endogenous

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-YJR129c

   Plasmid

   #85118

   Purpose

   bacterial expression of YJR129c

   Depositor

   Pal Falnes

   Article

   Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

   Insert

   EFM3 (EFM3 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-R1ACR_6367-EYFP

    Plasmid

    #111080

    Purpose

    Anion channelrhodopsin from Rhodomonas sp. for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin R1ACR_6367
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-RlACR_497-EYFP

    Plasmid

    #111078

    Purpose

    Anion channelrhodopsin from Rhodomonas lens for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin RlACR_497
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-FFSARG-ddFLN4-HIS-ybbr

    Plasmid

    #101240

    Purpose

    minimal SdrG binding peptide FFSARG with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    FFSARG peptide, ddFLN4 fingerprint
    

    Use

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4

    Promoter

    T7

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. YIp128-P30-His-pol30(K127/164R)

    Plasmid

    #99545

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127/164R; Leu2 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    K127R, K164R

    Promoter

    POL30

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJB172

    Plasmid

    #86992

    Purpose

    CRISPR/Cas9 in fission yeast using fluoride selection and targetting pil1

    Depositor

    Julien Berro

    Article

    Fernandez et al Yeast. 2016 Oct;33(10):549-557. doi: 10.

    Insert

    gRNA targeting pil1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pILGFPJ1

    Plasmid

    #83561

    Purpose

    Express yEGFP-ERG9 ER signal peptide under the control of TEF1 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

    Insert

    TEF1 promoter-yEGFP-ERG9 ER signal peptide
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTCW022

    Plasmid

    #83532

    Purpose

    pheromone mediated expression of UBiC, ARO4, and TKL1

    Depositor

    Claudia Vickers

    Article

    Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

    Insert

    pFUS1J2-UBiC-CYC1t-pFUS1J2-ARO4*-CYC1t-pFUS1J2-TKL1-CYC1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pFUS1J2

    Available since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTCW015

    Plasmid

    #83530

    Purpose

    UBiC and feedback resistant ARO4 expression from pheromone inducible FUS1J2 promoter

    Depositor

    Claudia Vickers

    Article

    Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

    Insert

    pFUS1J2-UBiC-CYC1t-pFUS1J2-ARO4*-CYC1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pFUS1J2

    Available since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PHBA03

    Plasmid

    #83537

    Purpose

    Constitutive TEF1 promoter mediated expression of the UBiC, ARO4*, and TKL1 genes

    Depositor

    Claudia Vickers

    Article

    Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

    Insert

    pTEF1-UBiC-CYC1t-pTEF1-ARO4*-CYC1t-pTEF1-TKL1-CYC1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HCKan-T-TEF2

    Plasmid

    #65340

    Purpose

    construction of TEF2 terminator in HCKan-P receiving vector

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    TEF2 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HCKan-P-TDH3

    Plasmid

    #65336

    Purpose

    construction of TDH3 promoter in HCKan-P receiving vector

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    TDH3 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HCKan-T-ADH1

    Plasmid

    #65339

    Purpose

    construction of ADH1 terminator in HCKan-P receiving vector

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    ADH1 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTD125 GFP:POP1

    Plasmid

    #71387

    Purpose

    localization of yeast RNase POP1 (RNase MRP binding protein) in yeast

    Depositor

    Mark Schmitt

    Article

    Gill et al J Cell Biol. 2006 Apr 10;173(1):35-45. E

    Insert

    POP1 (POP1 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    ACT1

    Available since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pBAD-TRP32

    Plasmid

    #71730

    Purpose

    inducible expression of TRP32 in E.coli

    Depositor

    Jere McBride

    Article

    Luo et al Infect Immun. 2008 Apr;76(4):1572-80. do

    Insert

    TRP32
    

    Use

    Tags

    6xHIS and Thioredoxin

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

24. HCKan-P-CYC1

    Plasmid

    #65338

    Purpose

    construction of CYC1 promoter in HCKan-P receiving vector

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    CYC1 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Gal4pBS2-Pleum

    Plasmid

    #59992

    Purpose

    Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 11, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. Tpa1(273-644)

    Plasmid

    #23141

    Depositor

    SeWon Suh

    Article

    Kim et al Nucleic Acids Res. 2009 Dec 29. ():.

    Insert

    Tpa1 C-terminal domain (TPA1 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-272

    Promoter

    Available since

    Sept. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

27. pRS416 Gal-RNQ1 L94A-YFP

    Plasmid

    #18692

    Depositor

    Susan Lindquist

    Article

    Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

    Insert

    Rnq1 L94A (RNQ1 Budding Yeast)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Leucine 94 mutated to Alanine (L94A)

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

28. pJLI-Sup35N-KDG6-C

    Plasmid

    #1238

    Depositor

    Susan Lindquist

    Article

    Liu et al Proc Natl Acad Sci U S A 2002 Dec 10;99

    Insert

    SUP35 (SUP35 Budding Yeast)
    

    Use

    Yeast integrative plasmid

    Tags

    Expression

    Mutation

    SUP35 middle region (aa124-253) was replaced with…

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

29. Ecm2 1-143

    Plasmid

    #169923

    Purpose

    WT Ecm2 mutated to introduce stop codon after AA 143 of Ecm2

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    Ecm2 1-143
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Ecm2 1-265

    Plasmid

    #169925

    Purpose

    WT Ecm2 mutated to introduce stop codon after AA 265 of Ecm2

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    Ecm2 1-265
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Ecm2 1-325

    Plasmid

    #169926

    Purpose

    WT Ecm2 mutated to introduce stop codon after AA 325 of Ecm2

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    Ecm2 1-325
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV MP-84 mRFP

    Plasmid

    #206926

    Purpose

    The micro promoter MP-84 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (84 bp) allows delivery of large genes in AAV.

    Depositor

    Markus Grompe

    Article

    Chai et al Mol Ther Methods Clin Dev. 2023 May 17;2

    Insert

    mRFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MP-84

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Tet-pLKO-puro

    Plasmid

    #21915

    Purpose

    3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.

    Depositor

    Dmitri Wiederschain

    Article

    Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLenti-EF1a-hChR2(H134R)-EYFP-WPRE

    Plasmid

    #20942

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    EF1alpha

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. ZJOM30

    Plasmid

    #133673

    Purpose

    Integration of ERG6-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on ER and lipid droplets.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-ERG6-GFP (ERG6 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Diffusive 41kDa

    Plasmid

    #201335

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    EGFP-2PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Diffusive 27kDa (EGFP)

    Plasmid

    #201362

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Diffusive 67kDa

    Plasmid

    #201338

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    EGFP-6PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Diffusive 34kDa

    Plasmid

    #201334

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    EGFP-1PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Diffusive 54kDa

    Plasmid

    #201337

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    EGFP-4PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge