59,474 results

1. pLX304-KDM6A

   Plasmid

   #203580

   Purpose

   Expresses wild-type KDM6A in mammalian cells.

   Depositor

   Abhishek Chakraborty, William Kaelin

   Article

   Chakraborty et al Science. 2019 Mar 15;363(6432):1217-1222

   Insert

   KDM6A (KDM6A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHH0103_PDCD6_EF-hand

   Plasmid

   #110009

   Purpose

   Protein expression and purification of PDCD6_EF-hand

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   PDCD6_EF-hand (PDCD6 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDONR223-FLT3

   Plasmid

   #23895

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   FLT3 (FLT3 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPonA-BI-Gl NORM-LacZA TER- LacZB

   Plasmid

   #86212

   Purpose

   beta Globin NMD construct with and without PTC under Bi-directional Ponasterone A inducible promoter

   Depositor

   Robert Singer

   Article

   Trcek et al Genes Dev. 2013 Mar 1;27(5):541-51. doi:

   Insert

   beta-Globin (HBB, Hbb Human, Mouse)
   

   Use

   Ponasterone a inducible promoter

   Tags

   lacZA or lacZB

   Expression

   Mammalian

   Mutation

   39TER in beta Globin in MCS II

   Promoter

   Ponasterone A inducible

   Available Since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCINeoFlag ALIX

   Plasmid

   #89859

   Purpose

   Expresses full length Flag-tagged ALIX in mammalian cells; internal ID WISP03-308

   Depositor

   Wesley Sundquist

   Article

   Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

   Insert

   ALIX (PDCD6IP Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGreenPuro-shRNA-Stx3S-C4

   Plasmid

   #99742

   Purpose

   3rd generation lentiviral vector expresses shRNA against human Stx3S with a GFP reporter.

   Depositor

   Thomas Weimbs

   Article

   Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-54

   Insert

   shRNA against Stx3S
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   H1

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GPR55-DuET

   Plasmid

   #213285

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR55 (GPR55 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-ING2

   Plasmid

   #13294

   Depositor

   Curtis Harris

   Article

   Pedeux et al Mol Cell Biol. 2005 Aug . 25(15):6639-48

   Insert

   ING2 (INGX Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pLX302 ID2-V5 puro

   Plasmid

   #83096

   Purpose

   Lentiviral vector for constitutive expression of human ID2 with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   ID2 (ID2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

10. pCAX-EGFP-CIP4

    Plasmid

    #178363

    Purpose

    Expresses CIP4 c-terminal labeled with EGFP in mammalian cells

    Depositor

    Erik Dent

    Article

    Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

    Insert

    Cdc42 Interacting Protein 4 (TRIP10 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MEK1-GFP

    Plasmid

    #14746

    Depositor

    Rony Seger

    Article

    Yao et al Oncogene. 2001 Nov 15. 20(52):7588-96.

    Insert

    MEK1 (MAP2K1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV human IRS-1 CAAX

    Plasmid

    #11238

    Depositor

    Ronald Kahn

    Article

    Kriauciunas et al Mol Cell Biol. 2000 Sep . 20(18):6849-59

    Insert

    IRS-1 (IRS1 Human)
    

    Tags

    CAAX

    Expression

    Mammalian

    Mutation

    C-terminal prenylation motif makes IRS-1 localize…

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

13. TRMT10C (RG9MTD1A-c113)

    Plasmid

    #110248

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    RG9MTD1 (TRMT10C Human)
    

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Promoter

    T7 - lacO

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. R777-E185 Hs.PLXNB1

    Plasmid

    #70469

    Purpose

    Gateway ORF clone of human PLXNB1 [NM_001130082.2] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PLXNB1 (PLXNB1 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCEP4-hERG1a

    Plasmid

    #53052

    Purpose

    Expresses alpha subunit of hERG1a K channel in mammalian cells

    Depositor

    Michael Sanguinetti

    Article

    Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Raptor_1 shRNA

    Plasmid

    #1857

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    Raptor (RPTOR Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-hsyn-GRAB_ATP1.0

    Plasmid

    #167577

    Purpose

    Express the ATP sensor GRAB_ATP1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

    Insert

    ATP sensor GRAB_ATP1.0 (P2RY1 Human)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS2+-H2B-pr-mEosFP

    Plasmid

    #141101

    Purpose

    H2B fused to pr-mEosFP for chromatin visualization and primed conversion

    Depositor

    Periklis Pantazis

    Article

    Welling et al Bio Protoc. 2020 Jun 5;10(11):e3645. doi

    Insert

    Histone 2B
    

    Tags

    pr-mEosFP

    Expression

    Mammalian

    Promoter

    simian CMV IE94

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SnailHA_pcDNA3

    Plasmid

    #31697

    Depositor

    Paul Wade

    Article

    Kajita et al Mol Cell Biol. 2004 Sep . 24(17):7559-66

    Insert

    Snail (SNAI1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TFORF3397

    Plasmid

    #144873

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZIK1 (ZIK1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge