159,258 results

1. c-Flag pcDNA3

   Plasmid

   #20011

   Purpose

   This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.

   Depositor

   Stephen Smale

   Article

   Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-CMV

   Plasmid

   #72265

   Purpose

   Express gene of interest under CMV promoter

   Depositor

   Kazuhiro Oka

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. HA-APP-mGFP

   Plasmid

   #196696

   Purpose

   Expresses APP695 with an HA tag an N-terminal for single molecule tracking and other measusers in mammalian cells

   Depositor

   Martino Calamai

   Article

   Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

   Insert

   Amyloid Precursor Protein 695 isoform (APP Human)
   

   Use

   Tags

   HA and mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMCB306-GFP-Rab29

   Plasmid

   #198472

   Purpose

   lentiviral expression of GFP-Rab29

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   GFP-Rab29 (RAB29 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. hAAVS1 TALEN Left

   Plasmid

   #52341

   Purpose

   TALEN vector for genomic targeting

   Depositor

   Su-Chun Zhang

   Article

   Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

   Insert

   AAVS1 TALEN Left
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-fDIO EYFP

   Plasmid

   #55641

   Purpose

   Flp-Dependent EYFP

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   EYFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. hFLT1

   Plasmid

   #83435

   Purpose

   human VEGFR1 with HA and FLAG tag

   Depositor

   Christie Thomas

   Article

   Raikwar et al Exp Cell Res. 2013 Oct 15;319(17):2578-8

   Insert

   human VEGFR1 (FLT1 Human)
   

   Use

   Tags

   Flag and Myc

   Expression

   Mammalian

   Mutation

   HA-tag was inserted 30 AA downstream of the FLT1 …

   Promoter

   CMV

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. hAAVS1 TALEN Right

   Plasmid

   #52342

   Purpose

   TALEN vector for genomic targeting

   Depositor

   Su-Chun Zhang

   Article

   Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

   Insert

   AAVS1 TALEN Right
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLCK-mScarlet3_C1

   Plasmid

   #189771

   Purpose

   Construct for labelling plasma membranes in mammalian cells using LCK-mScarlet3

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   LCK-mScarlet3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PfV-Sapphire-IRES-DSRed

    Plasmid

    #116934

    Purpose

    Mammalian lenti vector for expression of the 40nm GEM and DSRed

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    Pfv
    

    Use

    Lentiviral

    Tags

    Sapphire

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. LM627

    Plasmid

    #191551

    Purpose

    LM627_MSG-AGGA-promoter-ccdb-TTCC-SNAC-his

    Depositor

    David Baker

    Article

    Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

    Insert

    ccdb
    

    Use

    Tags

    SNAC-tag, HIS-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. CROPseq-puro-v2

    Plasmid

    #127458

    Purpose

    Entry plasmid for guide cloning

    Depositor

    Paul Blainey

    Article

    Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZac2.1-GfaABC1D-mCherry-hPMCA2w/b

    Plasmid

    #111568

    Purpose

    Expresses a plasma membrane calcium pump hPMCA2w/b

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Has Service

    AAV5

    Insert

    Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. SPLICS Mt-ER Long P2A

    Plasmid

    #164107

    Purpose

    Detect the long Mitochondria-Endoplasmic reticulum contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP Mt-ER Long P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCX-SpiCee

    Plasmid

    #140836

    Purpose

    mammalian expression of SpiCee, a buffer of calcium that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    SpiCee
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-SFFV-GFP1-10

    Plasmid

    #80409

    Purpose

    Expresses GFP1-10 in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    GFP1-10
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPV-Dual_promoter-EF1α-2xNLS-Cascade+Cas3-P (RD)

    Plasmid

    #204619

    Purpose

    Expression vector of Cascade and Cas3. Puromycin selectable.

    Depositor

    Akitsu Hotta

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cas7, Cas5, Cas8, Cas11, Cas6, and Cas3
    

    Use

    CRISPR

    Tags

    Each protein has C- and N-terminal SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSR43.6r

    Plasmid

    #63197

    Purpose

    Expresses CcaS, Ho1, and PcyA constitutively

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

    Inserts

    CcaS
    Ho1
    PcyA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-PE6c

    Plasmid

    #207853

    Purpose

    Mammalian expression of PE6c prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6c
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCFD5

    Plasmid

    #73914

    Purpose

    multiplex gRNA plasmid

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    dU6-3:tRNA:gRNA:tRNA:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6:3

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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