We narrowed to 7,507 results for: PAC

1. mCherry-FKBP-PIP4K2BD278K

   Plasmid

   #202735

   Purpose

   expresses recruitable fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   mCherry:FKBP1A (3-108):[GGSA]4GG:PIP4K2B (D280K) (PIP4K2B, FKBP1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mCherry-FKBP-PIP4K2AD273K

   Plasmid

   #202733

   Purpose

   expresses recruitable fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   mCherry:FKBP1A(3-108):[GGSA]4GG:PIP4K2A (D266K) (PIP4K2A, FKBP1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCENPBR pc861

   Plasmid

   #167569

   Purpose

   Human CENP-B (aa 1-169 containing DNA-binding domain) cloned into pDsRed1-N1 vector (Clontech).

   Depositor

   Cristina Cardoso

   Article

   Chagin et al Nucleus. 2019 Dec;10(1):231-253. doi: 10

   Insert

   Homo sapiens centromere protein B (CENPB Human)
   

   Use

   Tags

   Ds-Red

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET32a-hNRG1a

   Plasmid

   #199235

   Purpose

   Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

   Depositor

   Nilson Zanchin

   Article

   Ferreira et al Mol Biol Rep. 2022 Dec;49(12):11715-1172

   Insert

   Neuregulin (NRG1 Human)
   

   Use

   Tags

   TrxA-6xHis-S-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMeCP2miRFP670 pc3942

   Plasmid

   #167568

   Purpose

   The miRFP670-gene is ligated c-terminally to the rat meCP2-gene.

   Depositor

   Cristina Cardoso

   Article

   Chagin et al Nucleus. 2019 Dec;10(1):231-253. doi: 10

   Insert

   MeCp2 (Mecp2 Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. P2061

   Plasmid

   #186299

   Purpose

   Expresses LexA-HBD-B112 under the control of pAct1 and Cas9 under the control of LexA-HBD-B112+Beta-estradiol inducible promoter

   Depositor

   Joerg Stelling

   Article

   Azizoglu et al Curr Protoc. 2023 Jan;3(1):e647. doi: 10

   Inserts

   LexA-HBD-B112
   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   2xLexop-minimalCyc1 and PACT1

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. KTK_139

   Plasmid

   #180565

   Purpose

   Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Insert

   Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSEVA251_ Ptrc.x.tetO2::D-HicDH

   Plasmid

   #185456

   Purpose

   CDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid.

   Depositor

   Wolfgang Kroutil

   Article

   Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

   Insert

   D-HicDH from Lactobacillus paracasei DSM 20008
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptrc.x.tetO2

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pESC-LEU-IpgD

   Plasmid

   #183672

   Purpose

   Inducible Shigella flexneri IpgD for expression in yeast

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   IpgD
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. KTK_324

    Plasmid

    #180566

    Purpose

    Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX330 NLC-FKBP-Cas9

    Plasmid

    #162726

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX330 C-FKBP-Cas9

    Plasmid

    #162729

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSIN-Gapdh

    Plasmid

    #175938

    Purpose

    knockout mouse Gapdh

    Depositor

    Dan Littman

    Article

    Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

    Insert

    Gapdh gRNA (Gapdh Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5-FRT-TO-Orf6

    Plasmid

    #157726

    Purpose

    mammalian expression of untagged SARS-CoV-2 Orf6 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Orf6 (ORF6 SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pcDNA5-FRT-TO-Nsp15

    Plasmid

    #157720

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp15 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pcDNA5-FRT-TO-Nsp14

    Plasmid

    #157717

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pMXs_FLAG-Sfxn2 (CG6812)

    Plasmid

    #110643

    Purpose

    Retroviral expression of Flag-tagged Drosophila CG6812 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    CG6812 (CG6812 Fly)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon-optimized cDNA (no amino acid mutations)

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMXs_FLAG-Sfxn1-3 (CG11739)

    Plasmid

    #110642

    Purpose

    Retroviral expression of Flag-tagged Drosophila CG11739 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    CG11739 (CG11739 Fly)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon-optimized cDNA (no amino acid mutations)

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NES-PAmCherry-cPHx1

    Plasmid

    #116859

    Purpose

    PI(3,4)P2 biosensor

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    PLEKHA1 (PLEKHA1 Human)
    

    Use

    Tags

    PAmCherry

    Expression

    Mammalian

    Mutation

    amino acids 169-329

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5-puro-eGFP-HEC1-M4

    Plasmid

    #114036

    Purpose

    Expression of HEC1 M4 mutant tagged with GFP

    Depositor

    Geert Kops

    Article

    Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

    Insert

    GFP tagged - HEC1 -M4 (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M4 (K146E, R153E, K156E)

    Promoter

    CMV TetO

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA5-puro-eGFP-HEC1-M2

    Plasmid

    #114035

    Purpose

    Expression of HEC1 M2 mutant tagged with GFP

    Depositor

    Geert Kops

    Article

    Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

    Insert

    GFP tagged - HEC1 -M2 (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M2 (K81E, N87E, K89E)

    Promoter

    CMV TetO

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. sgGLDC_2

    Plasmid

    #72887

    Purpose

    CRISPR-Cas9 induced gene disruption

    Depositor

    David Sabatini

    Article

    Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

    Insert

    sgGLDC
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

23. sgGLDC_1

    Plasmid

    #72886

    Purpose

    CRISPR-Cas9 induced gene disruption

    Depositor

    David Sabatini

    Article

    Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

    Insert

    sgGLDC
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

24. pET28-p53 (1-73) (K24N)

    Plasmid

    #62079

    Purpose

    Expression of human p53 (1-73) (K24N) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Zhan et al Proteins. 2013 Oct;81(10):1738-47. doi:

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-73; K24N

    Promoter

    T7

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pTMB301_ZFcharm_Kv1_Prnp_SPM

    Plasmid

    #220840

    Purpose

    AAV genome expressing ZFcharm Kv1 targeting Prnp; single perfect match self-silencing binding site

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    ZFcharm Kv1
    

    Use

    AAV

    Tags

    HAtag

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRSFDuet-1_VNp-mNeongreen-Erythropoietin

    Plasmid

    #182423

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen-Erythropoietin fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-mNeongreen-eythropoietin (EPO Human)
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp) & mNeongreen

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Cbh_v5 AAV-CBE C-terminal

    Plasmid

    #137176

    Purpose

    AAV genome: expresses the C-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-CBE C-terminal; U6-protospacer
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Cbh

    Available since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Cbh_v5 AAV-CBE N-terminal

    Plasmid

    #137175

    Purpose

    AAV genome: expresses the N-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-CBE N-terminal; U6-protospacer
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Cas9 D10A

    Promoter

    Cbh

    Available since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-PE6d

    Plasmid

    #207854

    Purpose

    Mammalian expression of PE6d prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6d
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    M-MLVRTDRNAseHrt(T128N, V223Y, D200C)

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-pCALM1-sFLEx-HA-SpCas9-miniU6-sgRNAShank3

    Plasmid

    #213969

    Purpose

    AAV vector for encoding SpCas9 driven by pCALM1 promoter targeting Shank3 locus in the presence of Cre recombinase

    Depositor

    Zhonghua Lu

    Article

    Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

    Insert

    Shank3 sgRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSLQ9715: pHR-PGK-SV40_NLS-dCasMINI-V4-NFZ-c-Myc_NLS-mCherry-WPRE

    Plasmid

    #203221

    Purpose

    Expression of dCasMINI-V4-NFZ-mCherry in mammalian cells

    Depositor

    Stanley Qi

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCasMINI-V4-NFZ-mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-hSyn-FLEX-TeLC-P2A-dTomato

    Plasmid

    #159102

    Purpose

    Cre-dependent tetanus toxin light chain construct with nuclear-localized dTomato fluorophore for conditional silencing of neurons.

    Depositor

    Sandeep Datta

    Article

    Pashkovski et al Nature. 2020 Jul;583(7815):253-258. doi:

    Insert

    TeLC-P2A-NLS-dTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSynapsin

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pVSV-G

    Plasmid

    #138479

    Purpose

    Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particle.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    VSV-G envelop protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. His6-EGFP-PH-PLCD1

    Plasmid

    #183675

    Purpose

    PtdIns(4,5)P2 biosensor for purification from bacteria

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    PLCD1 (PLCD1 Human)
    

    Use

    Tags

    His6-EGFP

    Expression

    Bacterial

    Mutation

    Amino acids 11-140

    Promoter

    T7

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-CAG-SV40NLSf-GFP-3xmiR122-WPRE-HGHpA

    Plasmid

    #183775

    Purpose

    AAV genome with a CAG driven eGFP reporter with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

    Depositor

    Ben Deverman

    Article

    Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

    Insert

    NLS-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    CAG

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGFP-hPMLIII

    Plasmid

    #181903

    Purpose

    Fluorescently tagged PMLIII

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    hPMLIII (PML Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pENTR-AmCyan

    Plasmid

    #138481

    Purpose

    For cloning sgRNA flanked by two ribozyme elements, to subsequently cloned into PL-5LTR-GW-A vector.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    AmCyan
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV-PE6g

    Plasmid

    #207857

    Purpose

    Mammalian expression of PE6g prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6g
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Cas9(H840A, E471K, H99R, I632V, H721Y, R654C, D64…

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMV-PE6f

    Plasmid

    #207856

    Purpose

    Mammalian expression of PE6f prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6f
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PGKp-GFP-Empty

    Plasmid

    #174175

    Purpose

    Control lentiviral vector expressing GFP under control of the human PGK promoter.

    Depositor

    Rod Bremner

    Article

    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Aug. 19, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge