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MCHR1B_R (TAL 3009)
Plasmid#36001DepositorInsertTAL array targeting MCHR1B (mchr1b Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
MCHR1B_L (TAL 3008)
Plasmid#36000DepositorInsertTAL array targeting MCHR1B (mchr1b Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
TAL3477
Plasmid#47196DepositorInsertZebrafishCommunity-klf8-right (si:ch211-208m1.2 Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET15b/EYFP-GgVcl 1-258
Plasmid#46263DepositorInsertVcl 1-258 (VCL Chicken)
UseTagsEYFP and HisExpressionBacterialMutationaa 1-258 (VD1)PromoterT7Available sinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
140_pSKB3-GBD-LUMP
Plasmid#65972PurposeBacterial expression of GBD-LUMP protein, a sensor for GTP-bound Cdc42DepositorInsertLUMP
UseTagsGBD (GTPase binding domain from ACK1; residues 44…ExpressionBacterialMutationPromoterT7Available sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJK511
Plasmid#71591PurposeProduces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag, His222>Gln mutant (TpCSH6-H222Q)DepositorInsertcitrate synthase
UseTagsHis6ExpressionBacterialMutationchanges histidine-222 to glutaminePromoterT7 promoterAvailable sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 STAT6
Plasmid#67123PurposeStandard for cell-free expression benchmarking.DepositorInsertSTAT6 (STAT6 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TFDP3
Plasmid#67083PurposeStandard for cell-free expression benchmarking.DepositorInsertTFDP3 (TFDP3 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TFDP1
Plasmid#67084PurposeStandard for cell-free expression benchmarking.DepositorInsertTFDP1 (TFDP1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TFF1
Plasmid#67047PurposeStandard for cell-free expression benchmarking.DepositorInsertTFF1 (TFF1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA3 WT
Plasmid#66179PurposeExpresses the wild type of Sulfolobus solfataricus PCNA3DepositorInsertDNA polymerase sliding clamp 3
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA2 WT
Plasmid#66175PurposeExpresses the wild type of Sulfolobus solfataricus PCNA2DepositorInsertDNA polymerase sliding clamp 2
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSC-hTDG-K330A-IntE2
Plasmid#52271PurposeBacterial expression of human TDG-K330A C-terminally fused to GyrA intein, SUMO-E2 and GSTDepositorInserthTDG (TDG Human)
UseTagsGyrA-intein-Ubc9-GSTExpressionBacterialMutationK330A, SUMO acceptor site mutationPromoterT7Available sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 FL C214S
Plasmid#47086DepositorInsertAL-09 FL C214S (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationC214SPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET30 NHERF1
Plasmid#32854DepositorInsertsolute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)
UseTagsS16 and hisExpressionBacterialMutationnonePromoterT7Available sinceFeb. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
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TAL3577
Plasmid#47296DepositorInsertZebrafishCommunity-tetmethylcytosinedioxygenase3(previous si:ch211-220a11.1)-right
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
TAL3576
Plasmid#47295DepositorInsertZebrafishCommunity-tetmethylcytosinedioxygenase3(previous si:ch211-220a11.1)-left
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
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