-
Plasmid#192683PurposeLentiviral expression of multi gNAs targeting hMYOD1 promoter to activate human MYOD1 transcriptionDepositorInsertHuman MYOD1 activating gRNAs #1,2,3 (MYOD1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-MS2-HA-C1-MBP-DmBam_13-36_AG
Plasmid#148839PurposeMammalian Expression of DmBam_13-36DepositorInsertDmBam_13-36 (bam Fly)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha2 P35S:MS2:EDLL:Tnos (GB1738)
Plasmid#160622PurposeTU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain EDLLDepositorInsertP35s_MS2:EDLL_Tnos
UseTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xMS2 oligo-library based
Plasmid#158200PurposeCassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted from the oligo pool experiment.DepositorInsert10 binding sites of MS2 from oligo library experiment
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hNEUROD1 gRNA_1-MS2-Puro
Plasmid#192676PurposeLentiviral expression of sgRNA targeting hNEUROD1 promoter to activate human NEUROD1 transcriptionDepositorInsertHuman NEUROD1 activating gRNA #1 (NEUROD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-His-MS2BP-G3BP1-WT
Plasmid#136008PurposeWT G3BP1 inserted with MS2BP tagged on the N-terminus to use with the Tethering Assay (MS2)DepositorInsertG3BP1 (G3BP1 Human)
UseTagsMS2BPExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFRT-PonA-BI-Gl-WT-24xMS2-PTC-18xPP7 (WP)
Plasmid#189792PurposeExpress wild-type and PTC-containing beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cellsDepositorInsertbeta-globin (HBB Human, Mouse)
UseTagsExpressionMammalianMutationPromoterPonasterone A inducibleAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-mNeurog2 gRNA_1-MS2-Puro
Plasmid#192680PurposeLentiviral expression of sgRNA targeting mIL1RN promoter to activate mouse Neurog2 transcriptionDepositorInsertMouse Neurog2 activating gRNA #1 (Neurog2 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-EGFP-HsNanos2_6-22_X
Plasmid#147929PurposeMammalian Expression of HsNanos2_6-22DepositorInsertHsNanos2_6-22 (NANOS2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-ABE-3'UTR-sgRNA-2xMS2
Plasmid#132554PurposeVector plasmid expressing ABEmax and sgRNA scaffold with MS2 replacing the Tetraloop and loop 2DepositorInsertsABEmax
sgRNA, with Tetraloop and loop 2 replaced by MS2 aptamer
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ecDHFR(DD)-MS2CP-VPg(FCV)
Plasmid#167313PurposeTemplate pDNA to prepare DD-CaVT mRNADepositorInsertDD-CaVT
UseSynthetic BiologyTagsDYK-tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti pTERT sgRNA2(MS2) mCherry
Plasmid#171055PurposeTargeting of Vp64-dCas9 activation system to hTERT promoterDepositorInsertpTERT sgRNA2(MS2) mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMS28: pTns(PmcCAST)_ΔTniQ,ΔTnsD
Plasmid#168161PurposeInducible expression of PmcCAST TnsAB and TnsC proteins.DepositorInsertPmcCAST Tns proteins (TnsAB and TnsC)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLN34 - pBait-5GC[1xMS2]-McaS
Plasmid#222403Purposearabinose-induced synthesis of a hybrid RNA with an MS2 hairpin surrounded by a 5bp GC clamp with E coli McaS inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest.DepositorInsertMcaS (mcaS E. coli)
UseTags1xMS2 hairpin, 5GC clampExpressionBacterialMutationPromoterpBADAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSS1 - pBait-5GC[1xMS2]-empty
Plasmid#222405Purposearabinose-induced synthesis of an RNA containing an MS2 hairpin flanked by a 5GC clamp. Used as a three-hybrid control. For cloning purposes, see plasmid #222403.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (RAB7A-GAC site)
Plasmid#170140PurposeAAV vector carrying a MS2 adRNA targeting the RAB7A transcriptDepositorInsertMS2-RAB7A(GAC)-MS2
UseAAVTagsExpressionMammalianMutationPromoterHuman U6Available sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
SL3-MS2-gRNA GG backbone (MOBE3-4)
Plasmid#219949PurposeGolden gate backbone for mammalian expression of Sp-gRNA with SL3-MS2DepositorInsertgRNA-MS2-SL3 (S. pyogenes Cas9)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SMS2-TEV-Twin-Strep
Plasmid#202529PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 2 (SMS2) in mammalian cellDepositorInsertSGMS2 (SGMS2 Human)
UseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationdeleted stop codon (TGA)PromoterCAG and chicken β-actin promoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA11-pMa-sgTS3-17xMS2V5-sgTS3
Plasmid#199442PurposeCRISPR donor plasmid to insert 17XMS2V5 into the ribosomal DNA region in dependent of homology armsDepositorInsertHITI donor template
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-DmRoquin-del725-755_AE
Plasmid#148607PurposeMammalian Expression of DmRoquin-del725-755DepositorInsertDmRoquin-del725-755 (roq Fly)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-DmRoquin-del790-812_AE
Plasmid#148609PurposeMammalian Expression of DmRoquin-del790-812DepositorInsertDmRoquin-del790-812 (roq Fly)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsNot4_275-575-FFTLtoDAEE_AG
Plasmid#148756PurposeMammalian Expression of HsNot4_275-575-FFTLtoDAEEDepositorInsertHsNot4_275-575-FFTLtoDAEE (CNOT4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsNot4-del377-402_AG
Plasmid#148759PurposeMammalian Expression of HsNot4-del377-402DepositorInsertHsNot4-del377-402 (CNOT4 Human)
UseTagsExpressionMammalianMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsNot4-del377-424_AG
Plasmid#148760PurposeMammalian Expression of HsNot4-del377-424DepositorInsertHsNot4-del377-424 (CNOT4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsNot4-del400-428_AG
Plasmid#148761PurposeMammalian Expression of HsNot4-del400-428DepositorInsertHsNot4-del400-428 (CNOT4 Human)
UseTagsExpressionMammalianMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-Rluc-6xbsMS2-polyA-Malat_AC
Plasmid#148430PurposeMammalian Expression of 6xbsMS2-polyA-MalatDepositorInsert6xbsMS2-polyA-Malat
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-TISU-RLuc-6xbsMS2-8xlet7_AC
Plasmid#148435PurposeMammalian Expression of 6xbsMS2-8xlet7DepositorInsert6xbsMS2-8xlet7
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-TISU-RLuc-6xbsMS2-random_AC
Plasmid#148437PurposeMammalian Expression of 6xbsMS2-randomDepositorInsert6xbsMS2-random
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsNot1iso1_1-1084-shRNAres_AC
Plasmid#148411PurposeMammalian Expression of HsNot1iso1_1-1084-shRNAresDepositorInsertHsNot1iso1_1-1084-shRNAres (CNOT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsNot1iso1_1085-1317-shRNAres_AC
Plasmid#148412PurposeMammalian Expression of HsNot1iso1_1085-1317-shRNAresDepositorInsertHsNot1iso1_1085-1317-shRNAres (CNOT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsNot1iso1_1318-1605-shRNAres_AC
Plasmid#148414PurposeMammalian Expression of HsNot1iso1_1318-1605-shRNAresDepositorInsertHsNot1iso1_1318-1605-shRNAres (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsNot1iso1_1606-1840-shRNAres_AC
Plasmid#148415PurposeMammalian Expression of HsNot1iso1_1606-1840-shRNAresDepositorInsertHsNot1iso1_1606-1840-shRNAres (CNOT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsNot1iso1_1841-2376-shRNAres_AC
Plasmid#148416PurposeMammalian Expression of HsNot1iso1_1841-2376-shRNAresDepositorInsertHsNot1iso1_1841-2376-shRNAres (CNOT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsRck_296-472-QHTRtoAAAA_AC
Plasmid#148421PurposeMammalian Expression of HsRck_296-472-QHTRtoAAAADepositorInsertHsRck_296-472-QHTRtoAAAA (DDX6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsRck_296-472-RKKtoAAA_AC
Plasmid#148422PurposeMammalian Expression of HsRck_296-472-RKKtoAAADepositorInsertHsRck_296-472-RKKtoAAA (DDX6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsRck_296-472-RQNtoAAA_AC
Plasmid#148423PurposeMammalian Expression of HsRck_296-472-RQNtoAAADepositorInsertHsRck_296-472-RQNtoAAA (DDX6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-GQ-RLuc-6xbsMS2-A95-Malat_AC
Plasmid#148426PurposeMammalian Expression of 6xbsMS2-A95-MalatDepositorInsert6xbsMS2-A95-Malat
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsNanos2-del6-22_X
Plasmid#147939PurposeMammalian Expression of HsNanos2-del6-22DepositorInsertHsNanos2-del6-22 (NANOS2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-Fluc-5BoxB-6xbsMS2-HhR_V
Plasmid#147758PurposeInsect Expression of 5BoxB-6xbsMS2-HhRDepositorInsert5BoxB-6xbsMS2-HhR
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-EGFP-HsNanos1_1-39_X
Plasmid#147926PurposeMammalian Expression of HsNanos1_1-39DepositorInsertHsNanos1_1-39 (NANOS1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only