Skip to main content
Addgene
Showing: 601 - 650 of 2321 results

  1. BirA*-HA-NES

    Plasmid
    #214473
    Purpose
    Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.
    Depositor
    Natalie Ahn
    Article
    Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.
    Insert
    BirA(R118G)
    Use
    Lentiviral; Gateway
    Tags
    HA tag and NES (Nuclear Exclusion Sequence)
    Expression
    Mammalian
    Mutation
    R118G
    Promoter
    EF-1a
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214473-plasmid-map-sequence-id-425592

    Enlarge

  32. pmirGLO-Smad4-1719

    Plasmid
    #49383
    Purpose
    Insert is a fragment of the Smad4 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.
    Depositor
    Martine Roussel
    Article
    Murphy et al Cancer Res. 2013 Oct 21.
    Insert
    Smad family member 4 (SMAD4 Mouse, Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Human phosphoglycerate kinase (PGK) promoter
    Available since
    Oct. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    49383-plasmid-map-sequence-id-73406

    Enlarge

  44. pCRI007-pGEM-PgpdA-Cas12aScaffold-BsmbI-TtrpC

    Plasmid
    #140200
    Purpose
    Cloning vector for LbCas12a crRNAs ( Step 1 of the 2-step cloning alternative). Full PgpdA sequence is reconstituted when amplifying the expression cassette for cloning in a fungal vector.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    dLbCas12a crRNA scaffold
    Expression
    Mutation
    Promoter
    PgpdA
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140200-plasmid-map-sequence-id-310179

    Enlarge

  47. pMP498

    Plasmid
    #134998
    Purpose
    Clustered Reporter. Fluorescent reporter to be used with m6A readers and writers. (5xZF binding sites + 63xGATC sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor
    Depositor
    Ahmad Khalil
    Article
    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.
    Insert
    Clust. (5XZFBS 63XGATC)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor
    Use
    AAV and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    134998-plasmid-map-sequence-id-269998

    Enlarge

Showing: 601 - 650 of 2321 results