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LentiGuide-zeo

Plasmid

#160091

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and zeocin resistance marker

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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ZipGFP-Casp3

Plasmid

#81241

Purpose

A ZipGFP-based Caspase-3 protease reporter T2A mCherry

Depositor

Xiaokun Shu

Article

To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

Insert

ZipGFP with caspase cleavage sequence T2A mCherry

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-FLAG-mRuby2

Plasmid

#175294

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with mRuby2

Tags

mRuby2

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti CMV TRE3G Neo GFP-Lamin A wildtype

Plasmid

#118709

Purpose

Lentiviral TET-ON inducible GFP-Lamin A wildtype

Depositor

Tom Misteli

Article

Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

Insert

GFP-Lamin A human wildtype (LMNA Human)

Use

Lentiviral; Tet-on inducible

Tags

GFP

Expression

Mammalian

Promoter

CMV TRE3G (TET-ON)

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV4-ApoE4

Plasmid

#87087

Purpose

Expresses ApoE4 in Mammalian cells

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

Apolipoprotein E4 (APOE Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FuG-E

Plasmid

#67509

Purpose

This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type E envelope coating virus particle with NeuRet.

Depositor

Kazuto Kobayashi

Article

Kato et al J Neurosci Methods. 2014 Apr 30;227:151-

Insert

Fusion Glycoprotein type E

Use

Lentiviral

Tags

Fusion protein of Vesicular stomatitis Indiana vi…

Promoter

CAGGS

Available Since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pCI-SEP_NR2A

Plasmid

#23997

Depositor

Robert Malinow

Article

Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

Insert

NR2A (Grin2a Rat)

Tags

SEP

Expression

Mammalian

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pLV-TetO-hNGN2-eGFP-Puro

Plasmid

#79823

Purpose

Expresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.

Depositor

Kristen Brennand

Article

Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

Insert

hNGN2-P2A-eGFP-T2A-PuroR (NEUROG2 Human)

Use

Lentiviral

Promoter

TetO

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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CMV-SEAP

Plasmid

#24595

Depositor

Alan Cochrane

Insert

SEAP (ALPP Human)

Expression

Mammalian

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3 mHIF-2α MYC (P405A/P530V/N851A)

Plasmid

#44027

Depositor

Celeste Simon

Article

Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

Insert

mHIF-2α (P405A/P530V/N851A) (Epas1 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

P405A, P530V, N851A

Promoter

CMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA-Sumo2 WT

Plasmid

#48967

Purpose

Expresses HA SUMO2 Wild Type in mammalian cells

Depositor

Guy Salvesen

Article

Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

Insert

SUMO2 (SUMO2 Human)

Tags

HA

Expression

Mammalian

Promoter

T7

Available Since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only

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RFP-zyxin

Plasmid

#26720

Depositor

Anna Huttenlocher

Article

Bhatt et al J Cell Sci. 2002 Sep 1. 115(Pt 17):3415-

Insert

Zyxin (ZYX Human)

Tags

DsRed

Expression

Bacterial and Mammalian

Available Since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only

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HA-HIF2alpha-pcDNA3

Plasmid

#18950

Depositor

William Kaelin

Article

Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pET28a-EGFP-CNA35

Plasmid

#61603

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with EGFP

Depositor

Maarten Merkx

Article

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

EGFP and CNA35

Tags

His tags

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pINDUCER21 (ORF-EG)

Plasmid

#46948

Purpose

Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest

Depositor

Stephen Elledge, Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-D3cpv

Plasmid

#36323

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

D3cpv second generation cameleon (calcium sensor)

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only

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secreted BirA-Flag

Plasmid

#64395

Purpose

Flag-tagged biotinylation enzyme for co-expression with bio-proteins

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

BirA (birA E. coli)

Tags

Flag tag

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pHN924

Plasmid

#178885

Purpose

Expression of GST-His8-HRV3C

Depositor

Kazuhiro Abe

Insert

human rhinovirus 3C protease

Tags

GST, His8

Expression

Bacterial

Promoter

T7

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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HDAC5 Flag

Plasmid

#13822

Purpose

Mammalian expression of human histone deacetylase 5 with flag tag

Depositor

Eric Verdin

Article

Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

Insert

HDAC5 (HDAC5 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pET-28a_6H-MMLV_RT_D524N-6H

Plasmid

#166945

Purpose

Bacterial expression of M-MLV reverse transcriptase

Depositor

Robert Tjian

Article

Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

Insert

gag-pol

Tags

6X His

Expression

Bacterial

Mutation

D524N, H8Y

Promoter

T7

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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