165,307 results

1. HDAC8 Flag

   Plasmid

   #13825

   Depositor

   Eric Verdin

   Article

   Waltregny et al FASEB J. 2005 Jun . 19(8):966-8.

   Insert

   HDAC8 (HDAC8 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Available Since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFB2B

   Plasmid

   #195284

   Purpose

   Bicistronic HSV-TK and Puromycin resistance for positive/negative selection

   Depositor

   Scott Lowe

   Article

   Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

   Insert

   HSV-TK 2A Puro
   

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pINDUCER21 (ORF-EG)

   Plasmid

   #46948

   Purpose

   Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest

   Depositor

   Thomas Westbrook, Stephen Elledge

   Article

   Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFLAG_G495R MDA5

   Plasmid

   #216799

   Purpose

   Expresses a single point mutant of melanoma differentiation-associated protein 5

   Depositor

   Sun Hur

   Insert

   melanoma differentiation-associated protein 5 (IFIH1 Human)
   

   Expression

   Mammalian

   Mutation

   G495R

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-sadCas9-VP64

   Plasmid

   #115790

   Purpose

   An AAV vector expressing S. aureus dCas9 fused to VP64.

   Depositor

   Nadav Ahituv

   Article

   Matharu et al Science. 2018 Dec 13. pii: science.aau06

   Insert

   Sa-dCas9
   

   Use

   AAV

   Tags

   NLS and NLS-VP64

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR/D_creERt2

   Plasmid

   #27321

   Depositor

   Leonard Zon

   Article

   Mosimann et al Development. 2011 Jan . 138(1):169-77.

   Insert

   creERt2
   

   Use

   Multisite gateway middle entry vector

   Available Since

   Jan. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET29-bdSUMO-Anti-ALFA nanobody

   Plasmid

   #212311

   Purpose

   Expression of anti-ALFA nanobody

   Depositor

   Arthur Laganowsky

   Article

   Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

   Insert

   anti-ALFA nanobody
   

   Tags

   bdSUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF3318

   Plasmid

   #144794

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TFCP2 (TFCP2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmVen-RPA70-IRES-RPA32-IRES-RPA14

   Plasmid

   #211431

   Purpose

   mammalian expression of human RPA70 N-terminally fused to mVenus and RPA32 and RPA14

   Depositor

   Helmut Pospiech

   Insert

   RPA70 - IRES - RPA32 - IRES - RPA14 (RPA1, RPA2, RPA3 Human)
   

   Tags

   mVenus (on RPA70)

   Expression

   Mammalian

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pP{ELAV-GeneSwitch}

    Plasmid

    #83957

    Purpose

    Expresses conditional RU486-dependent GAL4 protein under control of the elav promotor

    Depositor

    Haig Keshishian

    Article

    Osterwalder et al Proc Natl Acad Sci U S A. 2001 Oct 23;98

    Insert

    fusion protein consisting of the GAL4-DBD, human progesterone receptor-ligand-binding domain, and P65-AD (GAL4, RELA Budding Yeast, Human)
    

    Expression

    Insect

    Promoter

    elav

    Available Since

    Dec. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3 2xFLAG-2xSTREP E2F4

    Plasmid

    #236439

    Purpose

    transient overexpression of E2F4 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F4 (E2F4 Human)
    

    Tags

    FLAG-FLAG-STREP-STREP

    Expression

    Mammalian

    Available Since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. V5-GFP-TurboID-KDEL_pLX208

    Plasmid

    #202020

    Purpose

    expresses GFP-TurboID in the mammalian ER lumen, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    V5-TurboID-KDEL
    

    Use

    Lentiviral

    Tags

    GFP and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-TO-hNIL BSD-mApple

    Plasmid

    #182313

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression, bsd selection, mApple

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Inserts

    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    bsd-T2A-mycNLS-mApple
    

    Tags

    T2A-mycNLS-mApple

    Expression

    Mammalian

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223-NEK7

    Plasmid

    #23395

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    NEK7 (NEK7 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETcon(-)

    Plasmid

    #41522

    Depositor

    Andrew Scharenberg

    Type

    Empty backbone

    Tags

    3x(G4S) Linker, G3S Linker, HA, Myc Tag, and Stuf…

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDGE347

    Plasmid

    #153228

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. METTL3 shRNA 1 tet pLKO puro

    Plasmid

    #162985

    Purpose

    Tet-inducible shRNA targeting human METTL3 #1

    Depositor

    Mark Rubin

    Article

    Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

    Insert

    methyltransferase like 3 (METTL3 Human)
    

    Use

    Lentiviral

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBIG1a

    Plasmid

    #80611

    Purpose

    pBIG1a - Step1 vector a (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. tdTomato-anti-AlfaTag nanobody

    Plasmid

    #171819

    Purpose

    Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

    Depositor

    Ikuo Wada

    Article

    Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

    Insert

    tdTomato-anti-AlfaTag nanobody
    

    Tags

    6xHis and SEP tag (6xD)

    Expression

    Bacterial

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMC_mClover3_BSDminus_F0

    Plasmid

    #184165

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mClover3
    bsd
    

    Use

    CRISPR

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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