161,273 results

1. circRNA-synIRES-R25-NanoLuc

   Plasmid

   #188116

   Purpose

   Template plasmid to transcribe circular RNA encoding NanoLuc

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   NanoLuc
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. p426*-SpSgH

   Plasmid

   #136993

   Purpose

   Helper plasmid for SpCas9 gRNA cloning and expression

   Depositor

   Huimin Zhao

   Article

   Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 human cofilin S3E

   Plasmid

   #50855

   Purpose

   expresses psuedo-phosporylated, non-activatable cofilin in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 S3E (CFL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S3E psuedo-phosporylated, non-activatable

   Promoter

   CMV

   Available since

   Feb. 5, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pET21-Streptavidin-K121R

   Plasmid

   #89880

   Purpose

   Streptavidin that has unimpaired biotin binding after dye labeling

   Depositor

   Mark Howarth

   Article

   Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

   Insert

   Streptavidin-K121R-Glutamate_Tag
   

   Use

   Tags

   6 glutamate tag (C terminal on insert)

   Expression

   Bacterial

   Mutation

   K121R

   Promoter

   Available since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-ERT2CreERT2

   Plasmid

   #13777

   Purpose

   Mammalian expression of conditionally active form of Cre recombinase (activated in response to tamoxifen)

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   ERT2-Cre-ERT2 fusion protein
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLAMP1-mCherry

   Plasmid

   #45147

   Depositor

   Amy Palmer

   Article

   Van Engelenburg et al Nat Methods. 2010 Apr;7(4):325-30. doi:

   Insert

   LAMP1-mCherry (LAMP1 Human, Synthetic)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV hSyn FLEx-OFF Kir2.1-2A-GFP

   Plasmid

   #161580

   Purpose

   Express Kir2.1 in cells not expressing Cre.

   Depositor

   Robert Malenka, Kevin Beier

   Article

   Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

   Insert

   Kir2.1-2A-GFP (Kcnj2 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP)

   Plasmid

   #215014

   Purpose

   Express an autophagy (LC3) reporter in mammalian cells (untested)

   Depositor

   Wade Harper

   Insert

   MAP1LC3B (Map1lc3b Rat)
   

   Use

   Lentiviral

   Tags

   HaloTag(7)-mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF2049

   Plasmid

   #143866

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PURA (PURA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. LiOn-CAG∞RFP

    Plasmid

    #154016

    Purpose

    Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase

    Depositor

    Jean Livet

    Article

    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

    Insert

    mRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H210P

    Promoter

    CAG

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. NUDT3A

    Plasmid

    #42348

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    NUDT3A (NUDT3 Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

12. EF1a_KLF7_P2A_Hygro_Barcode

    Plasmid

    #120493

    Purpose

    Barcoded lentiviral vector to express KLF7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF7 (KLF7 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. SPDK3860

    Plasmid

    #199245

    Purpose

    Modified Tobacco rattle virus (TRV) RNA2 vector for editing of Nicotiana benthamiana Phytoene desaturase (PDS) gene

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
    

    Use

    T-dna vector

    Tags

    Expression

    Mutation

    Promoter

    pPEBV

    Available since

    June 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tet-O-FUW-EGR1-P2A-mCherry

    Plasmid

    #203856

    Purpose

    Tet-inducible lentiviral plasmid expressing EGR1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    EGR1 (EGR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKV016

    Plasmid

    #64767

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, 3x-HA-GFP to a 3xT7-RFP tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Menendez-Benito et al Proc Natl Acad Sci U S A. 2013 Jan 2;110

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-lyso-pHluorin

    Plasmid

    #70113

    Purpose

    Expression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidification

    Depositor

    Christian Rosenmund

    Article

    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

    Insert

    lyso-pHluorin (Cd63 Mouse, Aequorea victoria)
    

    Use

    Tags

    HA-Tag and pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. PL752-EN1-LA-p2a-mTagBFP2nls-3xHAtag-hGHpA-PGK-puroR-EN1-RA

    Plasmid

    #191691

    Purpose

    For knocking p2a-mTagBFP2nls-3xHAtag into EN1 locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    EN1 homology arms and p2a-mTagBFP2nls-3xHAtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. p1177-X1-8

    Plasmid

    #38150

    Depositor

    David Waugh

    Article

    Austin et al Protein Expr Purif. 2011 May;77(1):53-61

    Insert

    Carboxypeptidase A
    

    Use

    Tags

    HHHHHHRR

    Expression

    Insect

    Mutation

    Promoter

    polyhedron

    Available since

    Oct. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCALNL-NaChBac

    Plasmid

    #125580

    Purpose

    Cre-dependent expression of NaChBac (FLAG-tagged) in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    NaChBac
    

    Use

    Cre/Lox

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKT2-ACVR1-G328V-IRES-Katushka

    Plasmid

    #135002

    Purpose

    Expresses ACVR1 with a G328V mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Mendez et al Clin Cancer Res. 2020 Aug 1;26(15):4080-

    Insert

    Activin A receptor, type I (ACVR1) G328V (ACVR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c.983G>T, p.G328V

    Promoter

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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