We narrowed to 18,983 results for: INO

1. pGL3-luciferase-reporter-CHAF1B-SVA

   Plasmid

   #202829

   Purpose

   pGL3 luciferase reporter with CHAF1B SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

   Depositor

   Matthew Anderson

   Article

   Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

   Insert

   CHAF1B intronic SVA
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SVA-lncRNA AK057321 shRNA 1A scramble control

   Plasmid

   #202835

   Purpose

   Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 1A

   Depositor

   Matthew Anderson

   Article

   Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

   Insert

   pLVX-SVA-lncRNA shRNA 1A scramble control
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EGFP-MYO10ΔF3

   Plasmid

   #194857

   Purpose

   Express EGFP-MYO10 with the F3 FERM domain lobes deleted (1-1951) in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

   Insert

   MYO10 ΔF3 (MYO10 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Truncated MYO10 construct. MYO10 amino acids 1-1…

   Promoter

   CMV

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. eSpCas9-EF-5Usp5

   Plasmid

   #174873

   Purpose

   CRISPR vector for generating Usp5 STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   sgRNA for mouse Usp5 (Usp5 Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. eSpCas9-EF-5Wnk1

   Plasmid

   #174874

   Purpose

   CRISPR vector for generating Wnk1 STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   sgRNA for mouse Wnk1 (Wnk1 Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Sandgrouse+R111Q–insD

   Plasmid

   #191229

   Purpose

   Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

   Inserts

   ATP1A1
   ATP1B1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   removed aspartic acid at 121 and replaced arginin…

   Promoter

   PH and p10

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. W118-1_eGFP

   Plasmid

   #180379

   Purpose

   lentiviral transduction of eGFP gene

   Depositor

   Huda Zoghbi

   Article

   Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

   Insert

   eGFP (eGFP )
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ABHD2-3'UTR-WT

   Plasmid

   #190094

   Purpose

   examine the activity of ABHD2 3'UTR

   Depositor

   Bowen Liu

   Article

   Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi:

   Insert

   ABHD2 3' UTR Wild Type (ABHD2 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti-ALDH1A3gRNA3

   Plasmid

   #189748

   Purpose

   The construct was used for expression of gRNA for knocking out of the ALDH1A3 gene in Cas9 expressing cells.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   ALDH1A3 gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 2xNLS-NLP-cMyc AspCas12a

    Plasmid

    #182120

    Purpose

    pET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    2xNLS-NLP-cMyc AspCas12a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCS2-mCherry-Cenexin-S796A

    Plasmid

    #186434

    Purpose

    Expresses mCherry Cenexin with S796A mutation in mammalian cells and zebrafish embryos

    Depositor

    Heidi Hehnly

    Article

    Aljiboury et al Mol Biol Cell. 2022 Aug 1;33(9):br14. do

    Insert

    Cenexin (ODF2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Changed Serine 796 to Alanine

    Promoter

    CMV IE94

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pETDuet1-LanCL2-H264A-C-Ter-His

    Plasmid

    #154187

    Purpose

    To express LanCL2-H264A in bacterial cells

    Depositor

    Wilfred van der Donk

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    LanCL2
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    His264->Ala264

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-15b-LanCL1-H219A

    Plasmid

    #154190

    Purpose

    To express LanCL1-H219A in bacterial cells

    Depositor

    Wilfred van der Donk

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    LanCL1
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    His219->Ala219

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET-15b-LanCL1-C322A

    Plasmid

    #154191

    Purpose

    To express LanCL1-C322A in bacterial cells

    Depositor

    Wilfred van der Donk

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    LanCL1
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Cys322->Ala322

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMAL-p2x-LanCL2

    Plasmid

    #154192

    Purpose

    To express MBP-LanCL2 in bacterial cells

    Depositor

    Wilfred van der Donk

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    LanCL2
    

    Use

    Tags

    Maltose binding protein (MBP)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA-Prom1 SV8 (-Ex19a)

    Plasmid

    #184012

    Purpose

    Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701).

    Depositor

    Peter Stoilov

    Article

    Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

    Insert

    Prom1 SV8(-Ex19a) (Prom1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of exon 19a (amino acids 696-701).

    Promoter

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pESC-LEU-IpgD

    Plasmid

    #183672

    Purpose

    Inducible Shigella flexneri IpgD for expression in yeast

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    IpgD
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET24a His6 GST PYL2(14-188)

    Plasmid

    #177842

    Purpose

    Bacterial Expression of PYL2

    Depositor

    Karsten Melcher

    Article

    Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

    Insert

    PYR1-like 2 (PYL2 Mustard Weed)
    

    Use

    Tags

    6xHis-GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TMD20

    Plasmid

    #163675

    Purpose

    Expresses a ST6GAL1 mutant with 20 amino acids in its TMD in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    For this partial length ST chimera, two restricti…

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TMD21

    Plasmid

    #163676

    Purpose

    Expresses a ST6GAL1 mutant with 21 amino acids in its TMD in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    For this partial length ST chimera, two restricti…

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TMD22

    Plasmid

    #163677

    Purpose

    Expresses a ST6GAL1 mutant with 22 amino acids in its TMD in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    For this partial length ST chimera, two restricti…

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TMD24

    Plasmid

    #163678

    Purpose

    Expresses a ST6GAL1 mutant with 24 amino acids in its TMD in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    For this partial length ST chimera, two restricti…

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. F9F17F-GFP

    Plasmid

    #163679

    Purpose

    Expresses a TFR mutant with truncated cytosolic tail and TMD in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    transferrin receptor mutant (TFRC Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    This chimera contains 9 amino acids from the cyto…

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEX-4T Doc2beta C2AB(125) H158C

    Plasmid

    #134691

    Purpose

    Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Double C2 protein beta (Doc2b Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    H158C, C145A, C217A, C249A, C290A, C338A, C387A

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGEX-4T Doc2beta C2AB(125) F222C

    Plasmid

    #134692

    Purpose

    Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Double C2 protein beta (Doc2b Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    F222C, C145A, C217A, C249A, C290A, C338A, C387A

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEX-4T Doc2beta C2AB(125) A298C

    Plasmid

    #134693

    Purpose

    Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Double C2 protein beta (Doc2b Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    A298C, C145A, C217A, C249A, C290A, C338A, C387A

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pPodA11

    Plasmid

    #162843

    Purpose

    Expression protein designed PodA10 from a TEV cleavable IPTG inducible plasmid with ampicillin resistance

    Depositor

    Dianne Newman

    Article

    VanDrisse et al Proc Natl Acad Sci U S A. 2021 Mar 23;11

    Insert

    Protein Designed Pyocyanin demethylase
    

    Use

    Tags

    TEV-cleavable 6x-His tag

    Expression

    Bacterial

    Mutation

    A53N, I73T, A87V, M99V, A129T from WT PodA

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDECKO_mCherry_NFE2

    Plasmid

    #140105

    Purpose

    CRISPR-Cas9 Knock down

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDECKO_mCherry_TREML2

    Plasmid

    #140106

    Purpose

    CRISPR-Cas9 Knock down

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDECKO_mCherry_LINC02432

    Plasmid

    #140107

    Purpose

    CRISPR-Cas9 library validation

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDECKO_mCherry_MIR3945HG

    Plasmid

    #140108

    Purpose

    CRISPR-Cas9 library validation

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDECKO_mCherry_Intergenic

    Plasmid

    #140100

    Purpose

    CRISPR-Cas9 library validation (negative control)

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDECKO_mCherry_FURIN

    Plasmid

    #140103

    Purpose

    CRISPR-Cas9 library validation

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. KIF13B

    Plasmid

    #163907

    Purpose

    Full length mouse KIF13B for purification from insect cells

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    kif13b (Kif13b Mouse)
    

    Use

    Tags

    Cleavable 6 x HIS tag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. KIF13B 1-584

    Plasmid

    #163908

    Purpose

    Motile fragment of mouse KIF13B (aa 1-584) for purification from insect cells.

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    kif13b (Kif13b Mouse)
    

    Use

    Tags

    Cleavable 6 x HIS tag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMAL-c2X-ORF24-NTD-3L_A

    Plasmid

    #138466

    Purpose

    Expresses MBP-tagged ORF24-NTD 3L_A in bacteria

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    ORF24 (ORF24 KSHV (HHV-8))
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Amino acids 1-201; amino acids L73-75 mutated to …

    Promoter

    tac

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSHDY*in_PpetE:BCD2:AgBIS:Flag

    Plasmid

    #133971

    Purpose

    Heterologous, copper-inducible expression of (E)-α-bisabolene synthase from Abies grandis (AgBIS) in Synechocystis sp. PCC 6803. The AgBIS CDS is fused to a C-termial Flag-tag.

    Depositor

    Pia Lindberg

    Article

    Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.

    Insert

    AgBIS:Flag
    

    Use

    Synthetic Biology

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    inserted gene is codon optimized for Synechocyst…

    Promoter

    PpetE:BCD2

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSHDY*in_PpetE:BCD2:MrBBS:Strep

    Plasmid

    #133973

    Purpose

    Heterologous, copper-inducible expression of (-)-α- bisabolol synthase from Matricaria recutita (MrBBS) in Synechocystis sp. PCC 6803. The MrBBS CDS is fused to a C-termial StrepII-tag.

    Depositor

    Pia Lindberg

    Article

    Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.

    Insert

    MrBBS:StrepII
    

    Use

    Synthetic Biology

    Tags

    StrepII

    Expression

    Bacterial

    Mutation

    inserted gene is codon optimized for Synechocyst…

    Promoter

    PpetE:BCD2

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. SNX30-PX (86-205)

    Plasmid

    #119109

    Purpose

    Bacterial expression of human phox homology (PX) domain, SNX30-PX (86-205)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    SNX30-PX (86-205) (SNX30 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. AdMKP-3 S159A

    Plasmid

    #131520

    Purpose

    Expresses MKP-3 with S159A mutation in mammalian cells

    Depositor

    Haiyan Xu

    Article

    Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

    Insert

    Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
    

    Use

    Adenoviral

    Tags

    No

    Expression

    Mutation

    changed Serine 159 to Alanine

    Promoter

    CMV

    Available since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pLenti-CMV-TIRAP-cGASΔN-HA

    Plasmid

    #130921

    Purpose

    Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the TIRAP N terminus (aa1-85); Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    TIRAP-ΔNcGAS
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    cGAS N terminus (aa1-159) replaced with the N ter…

    Promoter

    CMV

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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42. pTAMAHISTEV_Ab-PirA

    Plasmid

    #128959

    Purpose

    expresses Acinetobacter baumannii PirA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

    Insert

    ferric enterobactin receptor PirA
    

    Use

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed

    Promoter

    T7

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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43. FLAG-HA-RNF68 [dRING]

    Plasmid

    #126549

    Purpose

    Expresses FLAG-HA-tagged RNF68 [dRING mutant] in mammalian cells

    Depositor

    Michele Pagano

    Article

    Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

    Insert

    RNF68 (PCGF1 Human)
    

    Use

    Retroviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    RING domain deletion mutant [dRING mutation is a …

    Promoter

    LTR

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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44. FLAG-HA-RNF68 [Y252D]

    Plasmid

    #126550

    Purpose

    Expresses FLAG-HA-tagged RNF68 [Y252D] mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

    Insert

    RNF68 (PCGF1 Human)
    

    Use

    Retroviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Y252D point mutant

    Promoter

    LTR

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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45. pDONR223 GABARAPL1 no-stop

    Plasmid

    #123210

    Purpose

    Gateway entry clone encoding human GABARAPL1 lacking stop codon, suitable for C-terminal tagging

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    

    Use

    Gateway entry vector / entry clone

    Tags

    Expression

    Mutation

    Stop codon removed

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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46. pDONR223 GABARAPL2 no-stop

    Plasmid

    #123211

    Purpose

    Gateway entry clone encoding human GABARAPL2 lacking stop codon, suitable for C-terminal tagging

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
    

    Use

    Gateway entry vector / entry clone

    Tags

    Expression

    Mutation

    Stop codon removed

    Promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. pUCHR-mClover-smAID-P2A-CD5Flag2-bglpA

    Plasmid

    #110654

    Purpose

    Lentiviral vector expressing cell surface Flag-tag and monomeric GFP C-terminaly fused with auxin inducible degron

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

    Insert

    mClover-AID-P2A-CD5Flag2-bglpA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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48. pUCHR-mClover-smAID-P2A-CD5cmyc2-bglpA

    Plasmid

    #110656

    Purpose

    Lentiviral vector expressing cell surface c-Myc-tag and monomeric GFP C-terminaly fused with auxin inducible degron

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

    Insert

    mClover-AID-P2A-CD5cmyc2-bglpA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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49. pEJS592-pCSDest2-AcrIIC4Hpa-BFPv2-IRES

    Plasmid

    #113438

    Purpose

    Expresses type II-C anti-CRISPR protein from H. parainfluenzae and a BFP from IRES in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Inserts

    Type II-C anti-CRISPR
    mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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50. pEJS593-pCSDest2-AcrIIC5Smu-BFPv2-IRES

    Plasmid

    #113439

    Purpose

    Expresses type II-C anti-CRISPR protein from S. muelleri and a BFP from IRES in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Inserts

    Type II-C anti-CRISPR
    mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge