We narrowed to 6,963 results for: RAP

1. pFOSGE_CUS1B08

   Plasmid

   #71087

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   MED7 (MED7 Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pFOSGE_CUS1D07

   Plasmid

   #71085

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   MED10 (MED10 Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pFOSGE_COF2G03

   Plasmid

   #70882

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   TfIIFalpha (TfIIFalpha Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCMV CEPIA2mt

   Plasmid

   #58218

   Purpose

   Green fluorescent indicator for calcium imaging in the mitochondria

   Depositor

   Masamitsu Iino

   Article

   Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

   Insert

   CEPIA2mt
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   GCaMP2 N263Y E282V M339L

   Promoter

   CMV

   Available since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE

   Plasmid

   #32702

   Purpose

   Conditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.

   Depositor

   Hans Clevers

   Article

   Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

   Insert

   eGFP
   

   Use

   Cre/Lox and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRC_RR_VP1_r1c3

   Plasmid

   #65724

   Purpose

   Rescued infectious variant from pAAV2_RaPID library; mCherry insertion in place of the deleted amino acid sequence 453-GTTTQSR

   Depositor

   Junghae Suh

   Article

   Judd et al Mol Ther Nucleic Acids. 2012 Nov 13;1:e5

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AiP11390-pAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1390)

   Plasmid

   #163505

   Purpose

   DLX2.0 (3xCore version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain GABAergic interneurons. The enhancer core has 100% seq conservation between mouse and human

   Depositor

   Jonathan Ting, The Allen Institute for Brain Science

   Has Service

   AAV PHP.eB

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBetaGlobin

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCIS GEM-CEPIA1er

   Plasmid

   #58217

   Purpose

   Ratiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulum

   Depositor

   Masamitsu Iino

   Article

   Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

   Insert

   GEM-CEPIA1er
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   GEM-GECO1 E298D F359W D400E E407D

   Promoter

   CAG

   Available since

   July 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FKBP-NTEVp(WT)

   Plasmid

   #137831

   Purpose

   Cytosolic split-TEV protease chain with FKBP Rapamycin sensing domain, N-terminal WT TEV protease

   Depositor

   Joshua Leonard

   Article

   Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

   Insert

   FKBP-NTEVp(WT)
   

   Use

   Tags

   3x FLAG and NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FRB-CTEVp(WT)

    Plasmid

    #137832

    Purpose

    Cytosolic split-TEV protease chain with FRB Rapamycin sensing domain, C-terminal WT TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FRB-CTEVp(WT)
    

    Use

    Tags

    3x FLAG and NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CD9-mEGFP

    Plasmid

    #182864

    Purpose

    Localization: Tetraspanin/Membrane

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

    Insert

    human CD9 (CD9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-BE1

    Plasmid

    #73019

    Purpose

    Expresses BE1 with C-terminal NLS in mammalian cells

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBECKP-km

    Plasmid

    #117235

    Purpose

    A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Insert

    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Sth1Cas9n cytosine base editor

    Plasmid

    #194209

    Purpose

    Sth1Cas9-D9A-nickase_rAPOBEC1_cytosine base editor for RNA recording

    Depositor

    Chase Beisel

    Article

    Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

    Insert

    Sth1Cas9-D9A nickase
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Changed Aspartate 9 to Alanine.

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKM3

    Plasmid

    #154168

    Purpose

    Empty vector with FLAG tag expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-BE-zCas9

    Plasmid

    #101739

    Purpose

    expression of an optimized Cas9 for base-editing in zebrafish

    Depositor

    Shuo Lin

    Article

    Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

    Insert

    rAPOBEC1-XTEN-zCas9-UGI-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CMV

    Available since

    Jan. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG_EGFP-GFE3

    Plasmid

    #79869

    Purpose

    Expresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.

    Depositor

    Don Arnold

    Article

    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

    Insert

    GFP-GFE3
    

    Use

    Synthetic Biology

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Beta-actin

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGAN230

    Plasmid

    #131122

    Purpose

    reverse yTRAP, RNQ1-aTF reporting on tetR-mKate2

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SUP35

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSK221

    Plasmid

    #129249

    Purpose

    reverse yTRAP, tetR-responsive Neon reporter

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    pADH1(tetO2)-mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-Cerulean-Epac2-Venus

    Plasmid

    #138196

    Purpose

    FRET-based biosensor for monitoring Epac2 activation.

    Depositor

    Jin Zhang

    Article

    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

    Insert

    Cerulean-Epac2-Venus (Rapgef4 Synthetic, Mouse)
    

    Use

    Tags

    Cerulean and Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pR023_PIE-PUM2

    Plasmid

    #204819

    Purpose

    Expression PIE-PUM2 fusion protein in mammalian cells

    Depositor

    Xiaochang Zhang

    Article

    Ruan et al Nat Commun. 2023 Jun 6;14(1):3275. doi:

    Insert

    Pumilio 2 (PUM2 Human)
    

    Use

    Tags

    HA-Flag-rApobec1 and huADAR2dd

    Expression

    Mammalian

    Mutation

    Promoter

    chicken β-actin promoter

    Available since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLentiCRISPRv2-sgMaob-HepmCherry

    Plasmid

    #192829

    Purpose

    Expresses sgRNA targeting Maob and mCherry from hepatocyte-specific promoter

    Depositor

    Kristin Knouse

    Article

    Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

    Insert

    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for sgRNA and hepatocyte-specific promoter (HS…

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLentiCRISPRv2-Stuffer-HepmCherry

    Plasmid

    #192825

    Purpose

    Contains stuffer sequence into which sgRNAs can be cloned and expresses mCherry from hepatocyte-specific promoter

    Depositor

    Kristin Knouse

    Article

    Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

    Insert

    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJL-SaBE3

    Plasmid

    #85169

    Purpose

    Expresses SaBE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    SaBE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CP1028-CBEmax

    Plasmid

    #119802

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    rApobec1-nSpCas9 (CP1028) -UGI-UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FRB-mCherry-EEA1

    Plasmid

    #222419

    Purpose

    To express an early endosome "anchor" for rapamycin-induced heterodimerisation in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al bioRxiv 2024.05.31.596797

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Tags

    FRB-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CP1041-CBEmax

    Plasmid

    #119803

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    rApobec1-nSpCas9 (CP1041) -UGI-UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCB-CBEv1

    Plasmid

    #221138

    Purpose

    Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 1 without sgRNA construct. Expresses 3xF-HF-BE3.

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq-Ptac

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Lentiviral-FKBP-cpGFP-Nb39

    Plasmid

    #191498

    Purpose

    Rapamycin-dependent opioid sensor for opioid receptors (component 2)

    Depositor

    Wenjing Wang

    Article

    Kroning et al ACS Chem Biol. 2022 Aug 19;17(8):2212-22

    Insert

    FKBP-cpGFP-Nb39
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CP1249-CBEmax

    Plasmid

    #119804

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    rApobec1-nSpCas9 (CP1249) -UGI-UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCMV2-hNCOA6:V5

    Plasmid

    #63585

    Purpose

    A subunit of the Trithorax-related (Trr) methyltransferase complex.

    Depositor

    Kenneth Irvine

    Article

    Oh et al Cell Rep. 2014 Jul 24;8(2):449-59. doi:

    Insert

    NCOA6 (NCOA6 Human)
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pGAN147

    Plasmid

    #129202

    Purpose

    yeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backbone

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SUP35

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. CP1012-CBEmax

    Plasmid

    #119801

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    rApobec1-nSpCas9 (CP1012) -UGI-UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Lentiviral-MOR-FRB

    Plasmid

    #191497

    Purpose

    Rapamycin-dependent opioid sensor for the mu opioid receptor (component 1)

    Depositor

    Wenjing Wang

    Article

    Kroning et al ACS Chem Biol. 2022 Aug 19;17(8):2212-22

    Insert

    MOR-FRB
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBK-EE-BE3

    Plasmid

    #85175

    Purpose

    Expresses EE-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    EE-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Lentiviral-KOR-FRB

    Plasmid

    #191496

    Purpose

    Rapamycin-dependent opioid sensor for the kappa opioid receptor (component 1)

    Depositor

    Wenjing Wang

    Article

    Kroning et al ACS Chem Biol. 2022 Aug 19;17(8):2212-22

    Insert

    KOR-FRB
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-NGFP-VMAn-FKBP

    Plasmid

    #70225

    Purpose

    Expresses N-terminal portion of GFP along with VMA intein for protein splicing

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    eGFP
    

    Use

    Tags

    FK506- and rapamycin-binding protein (FKBP), Link…

    Expression

    Mammalian

    Mutation

    Changed Glu 125 to Ile, Deleted amino acids 129-2…

    Promoter

    CMV

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pLentiCRISPRv2-sgLmnb2-HepmCherry

    Plasmid

    #192830

    Purpose

    Expresses sgRNA targeting Lmnb2 and mCherry from hepatocyte-specific promoter

    Depositor

    Kristin Knouse

    Article

    Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

    Insert

    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for sgRNA and hepatocyte-specific promoter (HS…

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pB80-2xFKBP-L-mCherry-CAAX

    Plasmid

    #193713

    Purpose

    Rapalog-inducible coupling to plasma membrane (CAAX) via FKBP

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    2xFKBP-L-mCherry-CAAX
    

    Use

    Tags

    2xFKBP-mCherry

    Expression

    Mammalian

    Mutation

    mCherry: Met1 Deleted

    Promoter

    Chicken beta-actin

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLentiCRISPRv2-Stuffer-HepmTurquoise2

    Plasmid

    #192826

    Purpose

    Contains stuffer sequence into which sgRNAs can be cloned and expresses mTurquoise2 from hepatocyte-specific promoter

    Depositor

    Kristin Knouse

    Article

    Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

    Insert

    mTurquoise2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pGAN213

    Plasmid

    #129270

    Purpose

    Control for measuring background levels of yTRAP reporter protein

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    min pCYC1

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. FKBP-CD28TMD-NTEVp(WT)

    Plasmid

    #137826

    Purpose

    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal WT TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FKBP-CD28TMD-NTEVp(WT)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. NSarQ27G-VMAn-FKBP

    Plasmid

    #70230

    Purpose

    Expresses N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    alpha Sarcin (N-terminal portion)
    

    Use

    Tags

    FK506- and rapamycin-binding protein (FKBP), Link…

    Expression

    Mammalian

    Mutation

    Changed Gln 27 to Gly

    Promoter

    CMV

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

44. Pediculus humanus corporis PINK1 (143 - 575)

    Plasmid

    #110751

    Purpose

    Bacterial expression of PhPINK1 (crystallography)

    Depositor

    David Komander

    Article

    Schubert et al Nature. 2017 Dec 7;552(7683):51-56. doi:

    Insert

    Phum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)
    

    Use

    Tags

    N-His6-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pPBpuro-LDR

    Plasmid

    #173857

    Purpose

    Encoding a part of the rapamycin-induced dimerization system.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    a Lyn11-targeted FRB (LDR)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pOPTXLUC

    Plasmid

    #68504

    Purpose

    cyclic nucleotide reporter for bacteria or plant cells

    Depositor

    Helen Irving

    Article

    Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

    Insert

    OPTX promoter (AT1G33440 Mustard Weed)
    

    Use

    Tags

    luciferase

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    OPTX

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pcDNA3-P4:gs:nls:FKBP

    Plasmid

    #135984

    Purpose

    Expresses the P4 coiled coil fused to the FKBP rapamycin-inducible domain in mammalian cells

    Depositor

    Roman Jerala

    Article

    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

    Insert

    P4 coiled-coil fused to the FKBP domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. 3XFLAG-NSarQ27G-VMAn-FKBP

    Plasmid

    #70229

    Purpose

    Expresses FLAG tagged N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    alpha Sarcin (N-terminal portion)
    

    Use

    Tags

    3XFLAG, FK506- and rapamycin-binding protein (FKB…

    Expression

    Mammalian

    Mutation

    Changed Gln 27 to Gly

    Promoter

    CMV

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

49. CP1300-CBEmax

    Plasmid

    #119805

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    rApobec1-nSpCas9 (CP1300) -UGI-UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pAGH51

    Plasmid

    #107255

    Purpose

    HU-FRB construct (anchor) expressed in trans from the slr0168 neutral site of Synechocystis PCC6803. Used to anchor away any FKBP tagged target in presence of rapamycin.

    Depositor

    Erin O'Shea

    Article

    Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

    Insert

    HU-FRB
    

    Use

    Synthetic Biology

    Tags

    FRB

    Expression

    Mutation

    Promoter

    native HU promoter

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge