We narrowed to 23,108 results for: crispr

1. pJAT7

   Plasmid

   #204292

   Purpose

   For inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   3XP3-DsRed
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   3XP3

   Available Since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSF278

   Plasmid

   #167673

   Purpose

   Intermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX330-AAVS1

   Plasmid

   #227272

   Purpose

   Expresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting AAVS1 (AAVS1 Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCRIS-PITChv2-FBL

   Plasmid

   #63672

   Purpose

   PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   EGFP-2A-PuroR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Promoterless

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCBEclos-cbei2385-g2-opt

   Plasmid

   #118215

   Purpose

   CRISPR-mediated base editing in Clostridium

   Depositor

   Sheng Yang

   Article

   Li et al Biotechnol Bioeng. 2019 Feb 10. doi: 10.

   Insert

   Apobec1, Casp nickase, UG1-sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptm

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. qTAG-C-dTAG-Puro

   Plasmid

   #207727

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. qTAG-N-Puro-dTAG

   Plasmid

   #207694

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-dTAG cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pX330-BbsI-PITCh

   Plasmid

   #127875

   Purpose

   For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. qTAG-N-Puro-mScarlet

   Plasmid

   #207689

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mScarlet cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pMJ915

    Plasmid

    #69090

    Purpose

    Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

    Depositor

    Jennifer Doudna

    Article

    Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

    Insert

    cas9
    

    Use

    Tags

    MBP, hexa His, and two SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pXPR_050

    Plasmid

    #96925

    Purpose

    lentiviral expression of gRNA scaffold

    Depositor

    John Doench, David Root

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. qTAG-N-Blast-mStayGold

    Plasmid

    #227251

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mStayGold cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. qTAG-C-mStayGold-Blast

    Plasmid

    #227254

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. qTAG-C-mStayGold-Puro

    Plasmid

    #227253

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. qTAG-C-3xFLAG-Puro

    Plasmid

    #207728

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. qTAG-N-Puro-mStayGold

    Plasmid

    #227250

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mStayGold cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. qTAG-C-V5-Puro

    Plasmid

    #207730

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. qTAG-C-mStayGold-Zeo

    Plasmid

    #227255

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. qTAG-C-3xFLAG-Blast

    Plasmid

    #207717

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. qTAG-N-Zeo-mStayGold

    Plasmid

    #227252

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mStayGold cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. qTAG-C-3xFLAG-Zeo

    Plasmid

    #207739

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. qTAG-C-V5-Blast

    Plasmid

    #207719

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. qTAG-C-V5-Zeo

    Plasmid

    #207741

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pHAGE-TO-dCas9-3XGFP

    Plasmid

    #64107

    Purpose

    Expression of Sp dCas9-3XGFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp dCas9
    

    Use

    Lentiviral

    Tags

    3XsfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pN7CAST

    Plasmid

    #190661

    Purpose

    Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. qTAG-KO-PGK-Puro

    Plasmid

    #227264

    Purpose

    Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a PGK-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLBH531_MBP-Cas14a1 expression

    Plasmid

    #112500

    Purpose

    Expresses MBP fused to Cas14a1

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14a1
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. qTAG-C-mScarlet-Puro

    Plasmid

    #207722

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. qTAG-N-Blast-ultraID

    Plasmid

    #207682

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-ultraID cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pUAS-FRT-GFP-FRT-uM-dCBEevoCDA1

    Plasmid

    #205152

    Purpose

    Inducible expression of dCBEevoCDA1 under control of Gal4/UAS and FLP/FRT

    Depositor

    Michael Boutros

    Article

    Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

    Insert

    FRT-GFP-FRT-uORF-dCBEevoCDA1
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAST

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #113341

    Purpose

    Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

    Depositor

    Gavin Wright

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    VP64-dCas9-VP64-T2A-MS2-p65-HSF1
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMBP-AsCas12a

    Plasmid

    #113430

    Purpose

    Expresses 10xHis-MBP fused to AsCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    AsCas12a
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

    Plasmid

    #83946

    Purpose

    Cas9-carrying vector with kanMX marker

    Depositor

    Irina Borodina

    Article

    Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pX330A_D10A-1x2

    Plasmid

    #58772

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. LentiGuide Cherry

    Plasmid

    #170510

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Pavel Tolar

    Article

    Newman et al biorXiv 2021.03.02.433398

    Inserts

    S. pyogenes sgRNA cassette
    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a and hU6

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSGAb-km

    Plasmid

    #121999

    Purpose

    A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

    Depositor

    Quanjiang Ji

    Article

    Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

    Insert

    none
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSIBR004

    Plasmid

    #177667

    Purpose

    SIBR Intron 4 (Int4) interrupts the CDS of FnCas12a

    Depositor

    John van der Oost

    Article

    Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. qTAG-C-dTAG-Zeo

    Plasmid

    #207738

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pMRS-BG42-GFP

    Plasmid

    #220202

    Purpose

    mini-Tn7 GFP expression vector - pBG42 promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    monomeric superfolder green fluorescent protein
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG42

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMBP-FnCas12a

    Plasmid

    #113432

    Purpose

    Expresses 10xHis-MBP fused to FnCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    FnCas12a
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCE059-SiT-Cas12a-[Activ]

    Plasmid

    #128136

    Purpose

    Express both AsCas12a fused to [Activ] activator domain and CRISPR arrays on a single transcript

    Depositor

    Randall Platt

    Article

    Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

    Insert

    AsCas12a-[Activ]-Triplex
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. qTAG-N-Zeo-dTAG

    Plasmid

    #207705

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-dTAG cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

43. pMRS-LacI-GFP

    Plasmid

    #220198

    Purpose

    mini-Tn7 GFP expression vector - LacI/pLac promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    monomeric superfolder green fluorescent protein
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    LacI/pLac

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. BPK2014-AaCas12b

    Plasmid

    #121949

    Purpose

    E. coli expression, protein purification

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b
    

    Use

    Tags

    10xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. qTAG-C-miRFP670nano3-Blast

    Plasmid

    #207713

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

46. pEvolvR-enCas9-PolI5M

    Plasmid

    #113078

    Purpose

    Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    David Schaffer, John Dueber

    Article

    Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

    Insert

    enCas9-PolI5M
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pSpyC-GFP

    Plasmid

    #220191

    Purpose

    sgRNA expression vector - pBG42 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG42 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG42

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. qTAG-N-Puro-3xHA

    Plasmid

    #207696

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-3xHA cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

49. qTAG-N-Blast-miRFP670nano3

    Plasmid

    #207680

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-miRFP670nano3 cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

50. qTAG-C-miniTurbo-Puro

    Plasmid

    #207725

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge