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Showing: 6601 - 6650 of 16065 results

  9. Rtn2b-RHD272-472-GFP

    Plasmid
    #186606
    Purpose
    Encodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to GFP
    Depositor
    Gia Voeltz
    Article
    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6
    Insert
    Human Reticulon 2 isoform B amino acids 272 to 472 (RTN2 Human)
    Use
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    Met-Gly-Ser-Lys peptide added at N-terminus for c…
    Promoter
    CMV
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    186606-plasmid-map-sequence-id-369199

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  10. Rtn2b-RHD272-472-mNeon

    Plasmid
    #186605
    Purpose
    Encodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to mNeonGreen
    Depositor
    Gia Voeltz
    Article
    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6
    Insert
    Human Reticulon 2 isoform B amino acids 272 to 472 (RTN2 Human)
    Use
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    Met-Gly-Ser-Lys peptide added at N-terminus for c…
    Promoter
    CMV
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    186605-plasmid-map-sequence-id-369207

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  27. pJET-50-DExogron-mCherry-R11b

    Plasmid
    #179904
    Purpose
    Donor with homologous arms for Rab11b to knock in DExogron (=minilAA7-DExCon) mCherry module
    Depositor
    Patrick Caswell
    Article
    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:
    Insert
    RAB11B, member RAS oncogene family (RAB11B Human)
    Use
    CRISPR; Donor for homologous based knock in
    Tags
    mCherry
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TRE3GS
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    179904-plasmid-map-sequence-id-357197

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  37. FKBP-EGFP-RanGAP*

    Plasmid
    #175238
    Purpose
    Bacterial expression and purification, low affinity SUMOylation substrate that can recruited to FRB with rapamycin, point mutation F562A reduces affinity for E2, increasing the Km
    Depositor
    Michael Rosen
    Article
    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d
    Insert
    RANGAP1 (RANGAP1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Amino acid 562 F to A
    Promoter
    tac
    Available since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    175238-plasmid-map-sequence-id-345469

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  46. pRS405 SEC66-VAC8-GFP

    Plasmid
    #166840
    Purpose
    Expresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.
    Depositor
    Daniel Klionsky
    Article
    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554
    Insert
    VAC8 (VAC8, SEC66 Budding Yeast)
    Use
    Tags
    GFP and SEC66 transmembrane domain
    Expression
    Yeast
    Mutation
    SEC66 transmembrane domain
    Promoter
    VAC8 promoter
    Available since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166840-plasmid-map-sequence-id-329328

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  47. pAV-U6+27-Linear-2_PolyU(1)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid
    #159501
    Purpose
    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-2_PolyU(1)_HairpinDistance(10)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159501-plasmid-map-sequence-id-311777

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  48. pAV-U6+27-Linear-2_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid
    #159502
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-2_PolyU(4)_HairpinDistance(10)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159502-plasmid-map-sequence-id-311727

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  49. pAV-U6+27-Linear-3_PolyU(1)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid
    #159504
    Purpose
    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(1)_HairpinDistance(10)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159504-plasmid-map-sequence-id-311728

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  50. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid
    #159506
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(4)_HairpinDistance(10)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159506-plasmid-map-sequence-id-311732

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Showing: 6601 - 6650 of 16065 results